A Novel AP2/ERF Transcription Factor, OsRPH1, Negatively Regulates Plant Height in Rice

Ma, Ziming; Wu, Tao; Huang, Kai; Jin, Yongmei; Zhao, Li; Chen, Mojun; Yun, Sokyong; Zhang, Hongjia; Yang, Xue; Chen, Haoyuan; Bai, Huijiao; Du, Lin; Ju, Shanshan; Guo, Liping; Bian, Mingdi; Hu, Lanjuan; Du, Xinglin; Jiang, Wenzhu

doi:10.3389/fpls.2020.00709

Cited by 30 publications

(30 citation statements)

References 59 publications

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“…OsERF83 OX plants showed growth retardation and low grain yields ( Figure 3 , Figure S5, Table S1 ). Generally, plants exhibiting stress tolerance have been reported to show growth retardation [ 30 , 31 ] because of hypersensitivity in responding to stresses, directing resources to protection from stresses rather than growing or yielding, which is known as a trade-off. Thus, genes improving stress tolerance induce growth retardation under normal growth conditions when they are constitutively overexpressed.…”

Section: Discussionmentioning

confidence: 99%

Overexpression of OsERF83, a Vascular Tissue-Specific Transcription Factor Gene, Confers Drought Tolerance in Rice

Jung

Bang

Kim

et al. 2021

IJMS

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Abiotic stresses severely affect plant growth and productivity. To cope with abiotic stresses, plants have evolved tolerance mechanisms that are tightly regulated by reprogramming transcription factors (TFs). APETALA2/ethylene-responsive factor (AP2/ERF) transcription factors are known to play an important role in various abiotic stresses. However, our understanding of the molecular mechanisms remains incomplete. In this study, we identified the role of OsERF83, a member of the AP2/ERF transcription factor family, in response to drought stress. OsERF83 is a transcription factor localized to the nucleus and induced in response to various abiotic stresses, such as drought and abscisic acid (ABA). Overexpression of OsERF83 in transgenic plants (OsERF83OX) significantly increased drought tolerance, with higher photochemical efficiency in rice. OsERF83OXwas also associated with growth retardation, with reduced grain yields under normal growth conditions. OsERF83 is predominantly expressed in the vascular tissue of all organs. Transcriptome analysis revealed that OsERF83 regulates drought response genes, which are related to the transporter (OsNPF8.10, OsNPF8.17, OsLH1), lignin biosynthesis (OsLAC17, OsLAC10, CAD8D), terpenoid synthesis (OsTPS33, OsTPS14, OsTPS3), cytochrome P450 family (Oscyp71Z4, CYP76M10), and abiotic stress-related genes (OsSAP, OsLEA14, PCC13-62). OsERF83 also up-regulates biotic stress-associated genes, including pathogenesis-related protein (PR), wall-associated kinase (WAK), cellulose synthase-like protein E1 (CslE1), and LysM receptor-like kinase (RLK) genes. Our results provide new insight into the multiple roles of OsERF83 in the cross-talk between abiotic and biotic stress signaling pathways.

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Section: Discussionmentioning

confidence: 99%

Overexpression of OsERF83, a Vascular Tissue-Specific Transcription Factor Gene, Confers Drought Tolerance in Rice

Jung

Bang

Kim

et al. 2021

IJMS

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“…TA transactivation activity assay was performed according to the manufacturer's protocol (Clontech). The plasmid pBridge-OsWRKY50 was transformed into the yeast strain Y2HGold, in addition, pBridge-OsRPH1 was used as a positive control [32], and the empty vector pBridge was used as a negative control. Then, the transformants grown on a single-dropout media (SD/-Trp) were subcultured on double-dropout media (SD/-His/-Trp).…”

Section: Transactivation Activity and Yeast One-hybrid (Y1h) Assaysmentioning

confidence: 99%

Rice OsWRKY50 Mediates ABA-Dependent Seed Germination and Seedling Growth, and ABA-Independent Salt Stress Tolerance

Huang

Zhang

et al. 2021

IJMS

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Plant WRKY transcription factors play crucial roles in plant growth and development, as well as plant responses to biotic and abiotic stresses. In this study, we identified and characterized a WRKY transcription factor in rice, OsWRKY50. OsWRKY50 functions as a transcriptional repressor in the nucleus. The transcription of OsWRKY50 was repressed under salt stress conditions, but activated after abscisic acid (ABA) treatment. OsWRKY50-overexpression (OsWRKY50-OX) plants displayed increased tolerance to salt stress compared to wild type and control plants. The expression of OsLEA3, OsRAB21, OsHKT1;5, and OsP5CS1 in OsWRKY50-OX were much higher than wild type and control plants under salt stress. Furthermore, OsWRKY50-OX displayed hyposensitivity to ABA-regulated seed germination and seedling establishment. The protoplast-based transient expression system and yeast hybrid assay demonstrated that OsWRKY50 directly binds to the promoter of OsNCED5, and thus further inhibits its transcription. Taken together, our results demonstrate that rice transcription repressor OsWRKY50 mediates ABA-dependent seed germination and seedling growth and enhances salt stress tolerance via an ABA-independent pathway.

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“…Furthermore, Kavas et al indicated higher expression levels of PvAP2-ERF111, PvAP2-ERF119, PvAP2-ERF72, and PvAP2-ERF150 genes in common bean leaf tissues after salt treatment, suggesting the involvement of these genes in salt stress signal transduction [35]. Ma et al identified a novel AP2/ERF gene from rice, OsRPH1, that negatively controls plant height [36]. Additionally, a novel ERF gene, ZmERF105, was isolated from maize that positively controls resistance to Exserohilum turcicum [37].…”

Section: Introductionmentioning

confidence: 99%

The AP2/ERF Gene Family in Triticum durum: Genome-Wide Identification and Expression Analysis under Drought and Salinity Stresses

Faraji

Filiz

Kazemitabar

et al. 2020

Genes

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Members of the AP2/ERF transcription factor family play critical roles in plant development, biosynthesis of key metabolites, and stress response. A detailed study was performed to identify TtAP2s/ERFs in the durum wheat (Triticum turgidum ssp. durum) genome, which resulted in the identification of 271 genes distributed on chromosomes 1A-7B. By carrying 27 genes, chromosome 6A had the highest number of TtAP2s/ERFs. Furthermore, a duplication assay of TtAP2s/ERFs demonstrated that 70 duplicated gene pairs had undergone purifying selection. According to RNA-seq analysis, the highest expression levels in all tissues and in response to stimuli were associated with DRF and ERF subfamily genes. In addition, the results revealed that TtAP2/ERF genes have tissue-specific expression patterns, and most TtAP2/ERF genes were significantly induced in the root tissue. Additionally, 13 TtAP2/ERF genes (six ERFs, three DREBs, two DRFs, one AP2, and one RAV) were selected for further analysis via qRT-PCR of their potential in coping with drought and salinity stresses. The TtAP2/ERF genes belonging to the DREB subfamily were markedly induced under both drought-stress and salinity-stress conditions. Furthermore, docking simulations revealed several residues in the pocket sites of the proteins associated with the stress response, which may be useful in future site-directed mutagenesis studies to increase the stress tolerance of durum wheat. This study could provide valuable insights for further evolutionary and functional assays of this important gene family in durum wheat.

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A Novel AP2/ERF Transcription Factor, OsRPH1, Negatively Regulates Plant Height in Rice

Cited by 30 publications

References 59 publications

Overexpression of OsERF83, a Vascular Tissue-Specific Transcription Factor Gene, Confers Drought Tolerance in Rice

Overexpression of OsERF83, a Vascular Tissue-Specific Transcription Factor Gene, Confers Drought Tolerance in Rice

Rice OsWRKY50 Mediates ABA-Dependent Seed Germination and Seedling Growth, and ABA-Independent Salt Stress Tolerance

The AP2/ERF Gene Family in Triticum durum: Genome-Wide Identification and Expression Analysis under Drought and Salinity Stresses

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