A novel antimicrobial peptide YS12 isolated from Bacillus velezensis CBSYS12 exerts anti-biofilm properties against drug-resistant bacteria

Suchi, Suzia Aktar; Nam, Kyung Bin; Kim, Young Kyun; Tarek, Hasan; Yoo, Jin Cheol

doi:10.1007/s00449-023-02864-7

Cited by 7 publications

(7 citation statements)

References 59 publications

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“…A different AMP, 'YS12 ′ , was derived from Bacillus velezensis strain CBSYS12 via the Korean food kimchi. This showed strong antimicrobial activity against MRSA 4-5, VRE 82, E. coli, P. aeruginosa, and Mycobacterium smegmatis and inhibited 80% of biofilm formation by E. coli and P. aeruginosa [128]. Using rational design, Masadeh et al combined the alpha-helical parts of BMAP-28 (bovine cathelicidin) and LL-37 (human cathelicidin), generating the hybrid peptide MAA-41.…”

Section: 'Novel' Amp Typesmentioning

confidence: 99%

A Comprehensive Review of Recent Research into the Effects of Antimicrobial Peptides on Biofilms—January 2020 to September 2023

Fontanot,

Ellinger,

Unger

et al. 2024

Antibiotics

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Microbial biofilm formation creates a persistent and resistant environment in which microorganisms can survive, contributing to antibiotic resistance and chronic inflammatory diseases. Increasingly, biofilms are caused by multi-drug resistant microorganisms, which, coupled with a diminishing supply of effective antibiotics, is driving the search for new antibiotic therapies. In this respect, antimicrobial peptides (AMPs) are short, hydrophobic, and amphipathic peptides that show activity against multidrug-resistant bacteria and biofilm formation. They also possess broad-spectrum activity and diverse mechanisms of action. In this comprehensive review, 150 publications (from January 2020 to September 2023) were collected and categorized using the search terms ‘polypeptide antibiotic agent’, ‘antimicrobial peptide’, and ‘biofilm’. During this period, a wide range of natural and synthetic AMPs were studied, of which LL-37, polymyxin B, GH12, and Nisin were the most frequently cited. Furthermore, although many microbes were studied, Staphylococcus aureus and Pseudomonas aeruginosa were the most popular. Publications also considered AMP combinations and the potential role of AMP delivery systems in increasing the efficacy of AMPs, including nanoparticle delivery. Relatively few publications focused on AMP resistance. This comprehensive review informs and guides researchers about the latest developments in AMP research, presenting promising evidence of the role of AMPs as effective antimicrobial agents.

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Section: 'Novel' Amp Typesmentioning

confidence: 99%

A Comprehensive Review of Recent Research into the Effects of Antimicrobial Peptides on Biofilms—January 2020 to September 2023

Fontanot,

Ellinger,

Unger

et al. 2024

Antibiotics

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“…B. velezensis SK produced a surfactin with excellent antimicrobial activity against drug-resistant food-derived Bacillus cereus and the human pathogen Staphylococcus aureus at a concentration of 1600 AU/ml (Barale et al 2022). B. velezensis CBSYS12 produced a novel peptide YS12, which exhibited strong antimicrobial activity against gram-positive and gram-negative bacteria at a concentration of 6 to 12 µg/ml (Suchi et al 2023). Therefore, researchers need more efforts to explore antimicrobial compounds in B. velezensis.…”

Section: )mentioning

confidence: 99%

Analysis of the Genomic Sequences and Metabolites of Bacillus velezensis YA215

Yu,

Shen,

Chen

et al. 2024

Biochem Genet

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Discovering more novel antimicrobial compounds has become an keen research problem. In this study, YA215 genome was sequenced by the Illumina HiSeq + PacBio sequencing platform. Genome assembly was performed by Unicycler software and the gene clusters responsible for secondary metabolite biosynthesis were predicted by antiSMASH. The genome comprised 3976514 bp and had a 46.56% G + C content. 3809 coding DNA sequences, 27 rRNAs, 86 tRNAs genes and 79 sRNA were predicted. Strain YA215 was re-identi ed as Bacillus velezensis based on ANI and OrthoANI analysis. In the COG database, 23 functional groups from 3090 annotations were predicted. In the GO database, 2654 annotations were predicted. 2486 KEGG annotations linked 41 metabolic pathways. Glycosyl transferases, polysaccharide lyases, auxiliary activities, glycoside hydrolases, carbohydrate esterases and carbohydrate-binding modules were predicted among the 127 annotations in the CAZy database. AntiSMASH analysis predicted that strain B. velezensis YA215 boasted 13 gene clusters involved in synthesis of antimicrobial secondary metabolites including surfactin, fengycin, macrolactin H, bacillaene, di cidin, bacillibactin, bacilysin, and plantazolicin. Three of the gene clusters (gene cluster 5, gene cluster 9 and gene cluster 10) have the potential to synthesize unknown compounds. The research ndings have the potential to contribute signi cantly to the development of natural novel compounds with antimicrobial activity in B. velezensis YA215.

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“…The production and purification of the antimicrobial peptide YS12 derived from Bacillus velezensis CBSYS12 was described in our previous paper [15].…”

Section: Peptide Ys12 Production and Purificationmentioning

confidence: 99%

“…In the previous study, a novel antimicrobial peptide named YS12 was isolated from the traditional Korean food kimchi. Peptide YS12 from Bacillus velezensis CBSYS12 showed antimicrobial activity against different multi-drug resistant bacteria [15]. In many studies, it has been suggested that AMPs in combination with antibiotics offer rapid bactericidal effects and lower the chance of pathogens developing resistance [16,17].…”

Section: Introductionmentioning

confidence: 99%

Synergistic Effect, Improved Cell Selectivity, and Elucidating the Action Mechanism of Antimicrobial Peptide YS12

Suchi,

Lee,

Kim

et al. 2023

IJMS

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Antimicrobial peptides (AMPs) have attracted considerable attention as potential substitutes for traditional antibiotics. In our previous research, a novel antimicrobial peptide YS12 derived from the Bacillus velezensis strain showed broad-spectrum antimicrobial activity against Gram-positive and Gram-negative bacteria. In this study, the fractional inhibitory concentration index (FICI) indicated that combining YS12 with commercial antibiotics produced a synergistic effect. Following these findings, the combination of YS12 with an antibiotic resulted in a faster killing effect against bacterial strains compared to the treatment with the peptide YS12 or antibiotic alone. The peptide YS12 maintained its antimicrobial activity under different physiological salts (Na+, Mg2+, and Fe3+). Most importantly, YS12 exhibited no cytotoxicity towards Raw 264.7 cells and showed low hemolytic activity, whereas positive control melittin indicated extremely high toxicity. In terms of mode of action, we found that peptide YS12 was able to bind with LPS through electrostatic interaction. The results from fluorescent measurement revealed that peptide YS12 damaged the integrity of the bacterial membrane. Confocal laser microscopy further confirmed that the localization of peptide YS12 was almost in the cytoplasm of the cells. Peptide YS12 also exhibited anti-inflammatory activity by reducing the release of LPS-induced pro-inflammatory mediators such as TNF-α, IL-1β, and NO. Collectively, these properties strongly suggest that the antimicrobial peptide YS12 may be a promising candidate for treating microbial infections and inflammation.

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A novel antimicrobial peptide YS12 isolated from Bacillus velezensis CBSYS12 exerts anti-biofilm properties against drug-resistant bacteria

Cited by 7 publications

References 59 publications

A Comprehensive Review of Recent Research into the Effects of Antimicrobial Peptides on Biofilms—January 2020 to September 2023

A Comprehensive Review of Recent Research into the Effects of Antimicrobial Peptides on Biofilms—January 2020 to September 2023

Analysis of the Genomic Sequences and Metabolites of Bacillus velezensis YA215

Synergistic Effect, Improved Cell Selectivity, and Elucidating the Action Mechanism of Antimicrobial Peptide YS12

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