A novel antimicrobial peptide against dental-caries-associated bacteria

Chen, Long; Jia, Lili; Qiang, Zhimin; Zhou, Xi; Liu, Zhuqing; Li, Bingjie; Zhu, Zhentai; Wang, Fenwei; Chen, Yu; Zhang, Qian; Chen, Feng; Luo, Shi-Zhong

doi:10.1016/j.anaerobe.2017.05.016

Cited by 39 publications

(27 citation statements)

References 35 publications

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“…Several AMPs have good application potential. For instance, peptide ZXR-2 (FKIGGFIKKLWRSLLA) has shown potent activities against pathogenic bacteria of dental caries, Streptococcus mutans, Streptococcus sobrinus, and Porphyromonas gingivalis and peptide PAC-113 (Clinical trial identifier: NCT00659971) that has been sold over the counter in Taiwan for treating oral candidiasis (Chen L. et al, 2017).…”

Section: Current Progress and Application Of Antimicrobial Peptides Mmentioning

confidence: 99%

Antimicrobial Peptides: Classification, Design, Application and Research Progress in Multiple Fields

et al. 2020

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Antimicrobial peptides (AMPs) are a class of small peptides that widely exist in nature and they are an important part of the innate immune system of different organisms. AMPs have a wide range of inhibitory effects against bacteria, fungi, parasites and viruses. The emergence of antibiotic-resistant microorganisms and the increasing of concerns about the use of antibiotics resulted in the development of AMPs, which have a good application prospect in medicine, food, animal husbandry, agriculture and aquaculture. This review introduces the progress of research on AMPs comprehensively and systematically, including their classification, mechanism of action, design methods, environmental factors affecting their activity, application status, prospects in various fields and problems to be solved. The research progress on antivirus peptides, especially anti-coronavirus (COVID-19) peptides, has been introduced given the COVID-19 pandemic worldwide in 2020.

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Section: Current Progress and Application Of Antimicrobial Peptides Mmentioning

confidence: 99%

Antimicrobial Peptides: Classification, Design, Application and Research Progress in Multiple Fields

et al. 2020

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“…To improve these circumstances, taking natural AMPs as templates, many scholars have successfully designed and created many synthetic AMPs with promising antibacterial activity [35][36][37]. ese years, Chen et al [38] designed ZXR-2, Sullivan et al [27] synthesized C16G2, and Zhang et al [39] created DPS-PI, which were all synthetic AMPs that showed apparent antibacterial effect against the caries pathogenic bacteria, S. mutans. When compared with natural AMPs, synthetic AMPs can possess more efficient and broader-spectrum antibacterial activity and are not easy to produce resistance limitation, with lower cytotoxicity [34,35,40].…”

Section: Introductionmentioning

confidence: 99%

Antimicrobial Effect of a Peptide Containing Novel Oral Spray on Streptococcus mutans

Xiong

Chen

et al. 2020

BioMed Research International

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Objective. To investigate the antibacterial effect of a novel antimicrobial peptide containing oral spray GERM CLEAN on Streptococcus mutans (S. mutans) in vitro and further explore the related mechanisms at phenotypic and transcriptional levels. Methods. The disk diffusion method was used to preliminarily appraise the antimicrobial effect of GERM CLEAN. The minimal inhibitory concentration (MIC) of GREM CLEAN towards S. mutans was determined by the broth dilution method. S. mutans virulence-related phenotypic assays including initial adhesive assay, pH drop, exopolysaccharides (EPS), and biofilm formation measurements and quantitative real-time PCR (qRT-PCR) were further applied to detect the inhibitory mechanisms of GREM CLEAN at 1/2MIC. Results. The diameter (10.18 ± 1.744 mm) of inhibition zones formed by GERM CLEAN preliminarily indicated its inhibitory effect on the major cariogenic bacteria S. mutans. The minimal inhibitory concentration of GERM CLEAN on S. mutans was 100% mass fraction (the stock solution). The study of the antibacterial mechanism showed that GERM CLEAN had a certain inhibitory effect on the initial adhesion, acid production, extracellular polysaccharides (EPS) production, and biofilm formation of S. mutans. GERM CLEAN disturbed S. mutans biofilm physiology mainly through destruction of biofilm architecture and suppression of bacterial growth. The results of qRT-PCR further confirmed that the expression levels of EPS and lactic acid generation genes including gtfB, gtfC, gtfD, and ldh were significantly repressed by treating with GERM CLEAN, and this was consistent with our phenotypic results. Conclusion. The novel antimicrobial peptide containing oral spray GERM CLEAN has an anti-Streptococcus mutans effect and the inhibitory property may be due to suppression of the virulence factors of S. mutans including adhesive, acidogenicity, EPS, and biofilm formation.

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“…The next day, S. mutans and C. albicans suspension in BHI broth (180 µl/well, OD600nm= 0.1) was seeded into 96-well plates (Costar, Cambridge, MA) with 20 µl of the molecules (concentrations of 1.000, 100, 10, µM. DMSO 10%), as a negative control (death) 0.2% Chlorhexidine digluconate (Farpag®) was used, while the corresponding broth without molecules were used as a positive growth control, as well as those treated with DMSO 10% [57]. After incubation for 24 hours at 37°C shaking at 250 RPM, the absorbance was measured in an Epoch™ Microplate spectrophotometer (BioTek®) (OD600nm), to evaluate cell growth and to establish the minimum inhibitory concentration (MIC)…”

Section: Cytotoxicity and Antimicrobial Assaysmentioning

confidence: 99%

In silico Selection and in vitro Evaluation of Novel Inhibitors of Streptococcus Mutans Ag I/II Surface Protein

Cardona¹,

Padilla²,

Rivera³

et al. 2020

Preprint

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Streptococcus mutans is well known for having virulence factors associated with its cariogenic role, such as glucosyltransferases, which have been used as targets for the virtual screening of molecules with inhibitory capacity. The Antigen I/II of S. mutans is involved in the adhesion to the surface of the tooth and the bacterial co-aggregation in the biofilm formation, despite that, this protein has not been used as a target in a virtual strategy search for inhibitors. In this study we identified in silico and evaluated in vitro molecules with adhesion inhibitory potential on S. mutans Ag I/II. A virtual screening of 883,551 molecules was conducted, cytotoxicity analysis on fibroblast cells, S. mutans adhesion studies, scanning electron microscopy analysis for bacterial integrity, and molecular dynamics simulation were also performed. We have found three molecules (ZI-187, ZI-939, ZI-906) that were not cytotoxic and inhibited the adhesion of S. mutans to polystyrene microplates. Molecular dynamic simulation by 300 nanoseconds showed stability of the interaction between ZI-187 and Ag I/II (PDB: 3IPK). This work provides three new molecules that targets Ag I/II and have the capacity to inhibit in vitro the S. mutans adhesion on polystyrene microplates and provides a new computational line for the search and selection of safe inhibitory molecules against different pathogens.

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A novel antimicrobial peptide against dental-caries-associated bacteria

Cited by 39 publications

References 35 publications

Antimicrobial Peptides: Classification, Design, Application and Research Progress in Multiple Fields

Antimicrobial Peptides: Classification, Design, Application and Research Progress in Multiple Fields

Antimicrobial Effect of a Peptide Containing Novel Oral Spray on Streptococcus mutans

<em>In silico</em> Selection and <em>in vitro</em> Evaluation of Novel Inhibitors of <em>Streptococcus Mutans</em> Ag I/II Surface Protein

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