A novel antibiotic, linezolid, reduces intraperitoneal adhesion formation in the rat uterine horn model

Aytan, Hakan; Çalışkan, Ahmet; Yener, Tamer; Demirtürk, Fazlı; Aytan, Pelin; Yenişehirli, Aydan

doi:10.1080/00016340903002873

Cited by 14 publications

(8 citation statements)

References 29 publications

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“…Nevertheless, current adhesion-prevention strategies are largely limited to mechanical barriers, such as oxidized regenerated cellulose (Gynecare Interceed, Ethicon, Inc., Somerville, NJ) or sodium hyaluronate with carboxymethylcellulose (Saprafilm, Genzyme, Inc., Cambridge, MA). While the effectiveness of these barrier agents in clinical practice remains inconsistent (7-9) other studies indicate that limiting inflammatory processes and/or angiogenesis may provide efficacy in reducing adhesion formation (10-12). …”

Section: Introductionmentioning

confidence: 99%

Development and prevention of postsurgical adhesions in a chimeric mouse model of experimental endometriosis

Herington

Crispens

Carvalho-Macedo

et al. 2011

Fertility and Sterility

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Objective-To examine the impact of a recent surgery on development of endometriosis-related adhesions in a chimeric model and to determine the therapeutic efficacy of pioglitazone (PIO).Design-Human endometrial biopsies were maintained in estradiol (E) with or without PIO for 24 hrs prior to intraperitoneal injection into immunocompromised mice at multiple timepoints following peritoneal surgery. The presence and extent of adhesions was examined in animals relative to the initial establishment of experimental endometriosis. Setting-Medical School Research CenterPatients-Endometrial biopsies for experimental studies described herein were provided by normally cycling women without a medical history indicative of endometriosis or adhesions. Interventions-NoneMain Outcome Measure-Examination of the development of endometriosis-related adhesions in an experimental model.Results-Without therapeutic intervention, injection of E-treated human endometrial tissue into mice near the time of peritoneal surgery resulted in multiple adhesions and extensive endometriotic-like disease. In contrast, PIO treatment reduced adhesive disease and experimental endometriosis related to surgical injury.Conclusions-The presence of human endometrial tissue fragments in the peritoneal cavity of mice with a recent surgical injury promoted development of both adhesive disease and

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Section: Introductionmentioning

confidence: 99%

Development and prevention of postsurgical adhesions in a chimeric mouse model of experimental endometriosis

Herington

Crispens

Carvalho-Macedo

et al. 2011

Fertility and Sterility

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“…Multiple experimental studies using a variety of model systems have examined the efficacy of limiting inflammatory processes in an effort to reduce adhesion formation (12–15). For example, using a traditional animal model of adhesions, in which the peritoneal mesothelium is directly traumatized, investigators have demonstrated the effectiveness of sildenafil, resveratrol, and rosiglitazone in the inhibition of adhesion formation (16–19).…”

mentioning

confidence: 99%

Dietary fish oil supplementation inhibits formation of endometriosis-associated adhesions in a chimeric mouse model

Herington

Glore

Lucas

et al. 2013

Fertility and Sterility

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Objective To examine whether dietary fish oil supplementation reduces development of spontaneous endometriosis-associated adhesions using an established model. Design Laboratory-based study. Setting Medical center research laboratory. Patient(s)/Animal(s) Disease-free women of reproductive age and nude mice. Intervention(s) Women were not provided any intervention. Mice were randomized to receive fish oil supplementation or control diet. Main Outcome Measure(s) Experimental endometriosis was established in mice via injection of human endometrial tissue within 16 hours of ovariectomy. Mice were provided standard or menhaden fish oil–supplemented diets for ≥2 weeks before initiation of experimental endometriosis and until killing them 1 week later. At necropsy, mice were examined for the presence and extent of adhesions and endometriotic-like lesions. Tissues were excised and morphologically characterized. Result(s) Adhesions/lesions were reduced in mice provided with dietary fish oil compared with control animals. Leukocytes were more numerous within the adhesions/lesions of the mice maintained on the standard diet compared with animals provided with fish oil. As indicated by staining intensity, collagen deposition was greater at adhesion sites within control mice compared with fish oil–supplemented animals. Conclusion(s) Wound-healing associated with surgery created an inflammatory peritoneal microenvironment that promoted the development of both experimental endometriosis and adhesions in a murine model. Targeting excessive inflammation with fish oil may be an effective adjuvant therapy to reduce the development of postsurgical adhesions related to endometriosis.

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“…An array of antibiotics relevant to intra and post-operative care in sinus surgery were selected as treatments ( Table 1). Two oxazolidinone antibiotics were also selected due to emerging evidence that this class may facilitate a reduction in surgical adhesions (Aytan et al, 2009). Treatments were formulated using high concentration stock in cell media, at concentrations adapted from those reported in peer-reviewed literature when using common dosing regimens.…”

Section: Antibioticsmentioning

confidence: 99%

Antibiotics Affect ROS Production and Fibroblast Migration in an In-vitro Model of Sinonasal Wound Healing

Gouzos

Ramezanpour

Bassiouni

et al. 2020

Front. Cell. Infect. Microbiol.

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Introduction: Antibiotics are often administered to patients perioperatively and have been shown to affect ROS production of nasal cells in vitro, but their effect in the setting of active wound healing remains unclear. Reactive oxygen species (ROS) are known to play a significant role in wound healing. This study analyzed a broad array of antibiotics used after sinus surgery to assess their effect on wound healing and ROS production in vitro. It was hypothesized that ROS production would be affected by these antibiotics and there would be a negative relationship between ROS activity and cell migration speed. Methods: Monolayers of primary human nasal epithelial cells (HNEC) and primary fibroblasts were disrupted with a linear wound, treated with 10 different antibiotics or a ROS inhibitor and observed over 36 h in a controlled environment using confocal microscopy. ROS activity and migration speed of the wound edge were measured at regular intervals. The relationship between the two parameters was analyzed using mixed linear modeling. Results: Performing a linear scratch over the cell monolayers produced an immediate increase in ROS production of ∼35% compared to unscratched controls in both cell types. Incubation with mitoquinone and the oxazolidinone antibiotic linezolid inhibited ROS activity in both fibroblasts and HNEC in association with slowed fibroblast cell migration (p < 0.05). Fibroblast cell migration was also reduced in the presence of clarithromycin and mupirocin (p < 0.05). A significant correlation was seen between ROS suppression and cell migration rate in fibroblasts for mitoquinone and all antibiotics except for azithromycin and doxycycline, where no clear relationship was seen. Treatments that slowed fibroblast cell migration compared to untreated controls showed a significant correlation with ROS suppression (p < 0.05). Conclusion: Increased ROS production in freshly wounded HNEC and fibroblast cell monolayers was suppressed in the presence of antibiotics, in correlation with reduced fibroblast cell migration. In contrast, HNEC cell migration was not significantly affected by any of the antibiotics tested. This differential effect of antibiotics on fibroblast and HNEC migration might have clinical relevance by reducing adhesion formation without affecting epithelial healing in the postoperative setting.

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A novel antibiotic, linezolid, reduces intraperitoneal adhesion formation in the rat uterine horn model

Abstract: Linezolid was found to reduce intraperitoneal adhesion formation.

Cited by 14 publications

References 29 publications

Development and prevention of postsurgical adhesions in a chimeric mouse model of experimental endometriosis

Development and prevention of postsurgical adhesions in a chimeric mouse model of experimental endometriosis

Dietary fish oil supplementation inhibits formation of endometriosis-associated adhesions in a chimeric mouse model

Antibiotics Affect ROS Production and Fibroblast Migration in an In-vitro Model of Sinonasal Wound Healing

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