A novel animal model of primary blast lung injury and its pathological changes in mice

Meng, Xiangyan; Lu, Qian-Ying; Zhang, Jianfeng; Li, Junfeng; Shi, Mingyu; Huang, Siyu; Yu, Sifan; Zhao, Yanmei; Fan, Haojun

doi:10.1097/ta.0000000000003571

Cited by 5 publications

(8 citation statements)

References 21 publications

(49 reference statements)

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“…Early studies evaluating NETs in trauma used circulating cell-free DNA (cf-DNA) as a surrogate for NETosis (38,39). Meng et al (37) found that early after major trauma there is an increase in NETs followed by a decrease to a nadir above normal levels. In patients who subsequently developed sepsis, there was a second peak in NETs corresponding with the onset of infection (37).…”

Section: Neutrophil Ets In Trauma and Hemorrhagementioning

confidence: 99%

“…Furthermore, the cf-DNA levels were correlated with Injury Severity Score (ISS). Although there are some conflicting data, taken together, these studies indicate that cf-DNA may be promising as a marker that correlates with posttraumatic outcomes and sepsis (37,39,66).…”

Section: Cell-free Dnamentioning

confidence: 99%

“…Meng et al (37) found that early after major trauma there is an increase in NETs followed by a decrease to a nadir above normal levels. In patients who subsequently developed sepsis, there was a second peak in NETs corresponding with the onset of infection (37). Margraf et al (39) similarly demonstrated a posttraumatic increase in cf -DNA, which correlated with patients' Sequential Organ Failure Assessment and Multiple Organ Dysfunction Score scores.…”

Section: Trauma and Hemorrhagementioning

confidence: 99%

“…Neutrophil extracellular traps have been shown to be released following trauma in both in vitro studies and in critically ill trauma patients (36,37). Neutrophil extracellular traps have a complex structure containing azurophilic granules, NE, cathepsin G, MPO, DNA, and histones (16).…”

Section: Neutrophil Ets In Trauma and Hemorrhagementioning

confidence: 99%

“…Identification of NETosis was quantified in early studies using circulating cf-DNA levels as a surrogate and a potential biomarker for predicting clinical outcomes (37,39,66). Although cf-DNA is nonspecific and should not serve as a marker for NETosis without a confirmatory study, it may have potential as a clinical biomarker.…”

Section: Cell-free Dnamentioning

confidence: 99%

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Role of Peptidylarginine Deiminase and Neutrophil Extracellular Traps in Injuries: Future Novel Diagnostics and Therapeutic Targets

Ho¹,

Quan²,

Gauger³

et al. 2023

Shock

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Injuries lead to an early systemic inflammatory state with innate immune system activation. Neutrophil extracellular traps (NETs) are a complex of chromatin and proteins released from the activated neutrophils. Although initially described as a response to bacterial infections, NETs have also been identified in the sterile postinjury inflammatory state. Peptidylarginine deiminases (PADs) are a group of isoenzymes that catalyze the conversion of arginine to citrulline, termed citrullination or deimination. PAD2 and PAD4 have been demonstrated to play a role in NET formation through citrullinated histone 3. PAD2 and PAD4 have a variety of substrates with variable organ distribution. Preclinical and clinical studies have evaluated the role of PADs and NETs in major trauma, hemorrhage, burns, and traumatic brain injury. Neutrophil extracellular trap formation and PAD activation have been shown to contribute to the postinjury inflammatory state leading to a detrimental effect on organ systems. This review describes our current understanding of the role of PAD and NET formation following injury and burn. This is a new field of study, and the emerging data appear promising for the future development of targeted biomarkers and therapies in trauma.

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Section: Neutrophil Ets In Trauma and Hemorrhagementioning

confidence: 99%

Section: Cell-free Dnamentioning

confidence: 99%

Section: Trauma and Hemorrhagementioning

confidence: 99%

Section: Neutrophil Ets In Trauma and Hemorrhagementioning

confidence: 99%

Section: Cell-free Dnamentioning

confidence: 99%

See 3 more Smart Citations

Role of Peptidylarginine Deiminase and Neutrophil Extracellular Traps in Injuries: Future Novel Diagnostics and Therapeutic Targets

Ho¹,

Quan²,

Gauger³

et al. 2023

Shock

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Identification of potentially functional circRNAs and prediction of the circRNA-miRNA-hub gene network in mice with primary blast lung injury

Lu,

Li,

Zhao

et al. 2023

BMC Pulm Med

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Objectives Primary blast lung injury (PBLI) is the main cause of death in blast injury patients, and is often ignored due to the absence of a specific diagnosis. Circular RNAs (circRNAs) are becoming recognized as new regulators of various diseases, but the role of circRNAs in PBLI remain largely unknown. This study aimed to investigate PBLI-related circRNAs and their probable roles as new regulators in PBLI in order to provide new ideas for PBLI diagnosis and treatment. Methods The differentially expressed (DE) circRNA and mRNA profiles were screened by transcriptome high-throughput sequencing and validated by quantitative real-time PCR (qRT-PCR). The GO and KEGG pathway enrichment was used to investigate the potential function of DE mRNAs. The interactions between proteins were analyzed using the STRING database and hub genes were identified using the MCODE plugin. Then, Cytoscape software was used to illustrate the circRNA-miRNA-hub gene network. Results A total of 117 circRNAs and 681 mRNAs were aberrantly expressed in PBLI, including 64 up-regulated and 53 down-regulated circRNAs, and 315 up-regulated and 366 down-regulated mRNAs. GO and KEGG analysis revealed that the DE mRNAs might be involved in the TNF signaling pathway and Fanconi anemia pathway. Hub genes, including Cenpf, Ndc80, Cdk1, Aurkb, Ttk, Aspm, Ccnb1, Kif11, Bub1 and Top2a, were obtained using the MCODE plugin. The network consist of 6 circRNAs (chr18:21008725–21020999 + , chr4:44893533–44895989 + , chr4:56899026–56910247-, chr5:123709382–123719528-, chr9:108528589–108544977 + and chr15:93452117–93465245 +), 7 miRNAs (mmu-miR-3058-5p, mmu-miR-3063-5p, mmu-miR-668-5p, mmu-miR-7038-3p, mmu-miR-761, mmu-miR-7673-5p and mmu-miR-9-5p) and 6 mRNAs (Aspm, Aurkb, Bub1, Cdk1, Cenpf and Top2a). Conclusions This study examined a circRNA-miRNA-hub gene regulatory network associated with PBLI and explored the potential functions of circRNAs in the network for the first time. Six circRNAs in the circRNA-miRNA-hub gene regulatory network, including chr18:21008725–21020999 + , chr4:44893533–44895989 + , chr4:56899026–56910247-, chr5:123709382–123719528-, chr9:108528589–108544977 + and chr15:93452117–93465245 + may play an essential role in PBLI.

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Crosstalk between Inflammation and Hemorrhage/Coagulation Disorders in Primary Blast Lung Injury

Zhang

Shi

et al. 2023

Biomolecules

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Primary blast lung injury (PBLI), caused by exposure to high-intensity pressure waves from explosions in war, terrorist attacks, industrial production, and life explosions, is associated with pulmonary parenchymal tissue injury and severe ventilation insufficiency. PBLI patients, characterized by diffused intra-alveolar destruction, including hemorrhage and inflammation, might deteriorate into acute respiratory distress syndrome (ARDS) with high mortality. However, due to the absence of guidelines about PBLI, emergency doctors and rescue teams treating PBLI patients rely on experience. The goal of this review is to summarize the mechanisms of PBLI and their cross-linkages, exploring potential diagnostic and therapeutic targets of PBLI. We summarize the pathophysiological performance and pharmacotherapy principles of PBLI. In particular, we emphasize the crosstalk between hemorrhage and inflammation, as well as coagulation, and we propose early control of hemorrhage as the main treatment of PBLI. We also summarize several available therapy methods, including some novel internal hemostatic nanoparticles to prevent the vicious circle of inflammation and coagulation disorders. We hope that this review can provide information about the mechanisms, diagnosis, and treatment of PBLI for all interested investigators.

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A novel animal model of primary blast lung injury and its pathological changes in mice

Abstract: In this study, we established an animal model of PBLI using a self-made mini shock tube simulation device. Pathological changes of PBLI mice were characterized by mechanical damage and inflammatory response in lung tissue.

Cited by 5 publications

References 21 publications

Role of Peptidylarginine Deiminase and Neutrophil Extracellular Traps in Injuries: Future Novel Diagnostics and Therapeutic Targets

Role of Peptidylarginine Deiminase and Neutrophil Extracellular Traps in Injuries: Future Novel Diagnostics and Therapeutic Targets

Identification of potentially functional circRNAs and prediction of the circRNA-miRNA-hub gene network in mice with primary blast lung injury

Crosstalk between Inflammation and Hemorrhage/Coagulation Disorders in Primary Blast Lung Injury

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