A novel and highly efficient method for genetic transformation of fungi employing shock waves

Magaña-Ortíz, Denis; Coconi-Linares, Nancy; Ortiz-Vázquez, Elizabeth; Fernández, Francisco; Loske, Achim M.; Gómez-Lim, Miguel Á.

doi:10.1016/j.fgb.2013.03.008

Cited by 60 publications

(27 citation statements)

References 43 publications

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“…Most of the transformation techniques currently available for bacteria, e.g., electroporation (Schuster et al 2012 ), biolistic transformation (Lorito et al 1993 ;Te'o et al 2002 ), and transformation by the use of shock waves (Magaña-Ortíz et al 2013 ), also have been reported for Trichoderma . However, currently the most widely used and optimized procedures for Trichoderma strains are based on protoplasts and the Agrobacterium -mediated transformation (Cardoza et al 2006 ).…”

Section: General Methodsmentioning

confidence: 99%

Trichoderma Transformation Methods

2014

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Section: General Methodsmentioning

confidence: 99%

Trichoderma Transformation Methods

2014

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“…Water was used as a coupling media to transfer the shock waves with a peak positive pressure of approximately 38 MPa into the 100-μL suspension, contained in a 15 × 10 mm polyethylene bag. Details on shock wave-mediated transformation and the experimental device are given elsewhere [Coconi-Linares et al, 2014;Magaña-Ortiz et al, 2013]. All experiments were performed in 250-mL Erlenmeyer flasks containing 10 g of WB (14% cellulose, 35% hemicellulose, 26% starch, 12% protein, 11% lignin, w/w) or SB (51% cellulose, 9% hemicellulose, 36% lignin, w/w).…”

Section: Microorganism and Culture Conditionsmentioning

confidence: 99%

“…P. chrysosporium was transformed using underwater shock waves, as reported previously [CoconiLinares et al, 2014;Magaña-Ortiz et al, 2013]. We evaluated other primary transformants, in addition to those previously selected, picked at random and they all showed the same phenotype (data not shown).…”

Section: Introductionmentioning

confidence: 99%

Enhanced Delignification of Lignocellulosic Biomass by Recombinant Fungus <b><i>Phanerochaete chrysosporium</i></b> Overexpressing Laccases and Peroxidases

et al. 2018

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Ligninolytic enzyme production and lignin degradation are typically the rate-limiting steps in the biofuel industry. To improve the efficiency of simultaneous bio-delignification and enzyme production, Phanerochaete chrysosporium was transformed by shock wave-induced acoustic cavitation to co-overexpress 3 peroxidases and 1 laccase and test it on the degradation of sugarcane bagasse and wheat bran. Lignin depolymerization was enhanced by up to 25% in the presence of recombinant fungi in comparison with the wild-type strain. Sugar release on lignocellulose was 2- to 6-fold higher by recombinant fungi as compared with the control. Wheat bran ostensibly stimulated the production of ligninolytic enzymes. The highest peroxidase activity from the recombinant strains was 2.6-fold higher, whereas the increase in laccase activity was 4-fold higher in comparison to the control. The improvement of lignin degradation was directly proportional to the highest peroxidase and laccase activity. Because various phenolic compounds released during lignocellulose degradation have proven to be toxic to cells and to inhibit enzyme activity, a significant reduction (over 40%) of the total phenolic content in the samples treated with recombinant strains was observed. To our knowledge, this is the first report that engineering P. chrysosporium enhances biodegradation of lignocellulosic biomass.

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“…The exact mechanism responsible for shock wave-assisted cell permeabilization is still not clear, but there is evidence that it is due to shock wave-induced cavitation [2]. Shock-wave-mediated insertion of DNA has been reported in Escherichia coli, Pseudomonas aeruginosa, Salmonella [75][76][77], Aspergillus niger, Trichoderma reesei, Phanerochaete chrysosporium, and Fusarium oxysporum [78]. Several authors have published articles on shock-wave-mediated DNA delivery in eukaryotic cells and prokaryotes [79][80][81][82][83][84][85].…”

Section: Shock-wavesmentioning

confidence: 99%

Genetic Transformation of Cells using Physical Methods

Loske¹

2014

J Genet Syndr Gene Ther

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The production of transgenic cells is a routinely process that allows to insert genes from plants, fungi, viruses, bacteria and even animals into cells. Genetic transformation requires penetration of the transgene through the cell wall. This process is facilitated by biological, chemical or physical methods. We present a short review of the state of the art of physical methods used for genetic transformation. A general panorama of the traditional physical genetic transformation methods like electroporation, biolistic, and agitation with glass beads, vacuum infiltration, silicon carbide whisker, laser microbeams, ultrasound, and the newly promising technique of shock wave-mediated genetic transformation of cells are described. These techniques have been applied to transform cells of bacteria, algae, plants, fungi, and animals. In human cells, genetic transformation is currently used for DNA vaccines, tissue engineering, and cancer therapy.

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A novel and highly efficient method for genetic transformation of fungi employing shock waves

Cited by 60 publications

References 43 publications

Trichoderma Transformation Methods

Trichoderma Transformation Methods

Enhanced Delignification of Lignocellulosic Biomass by Recombinant Fungus <b><i>Phanerochaete chrysosporium</i></b> Overexpressing Laccases and Peroxidases

Genetic Transformation of Cells using Physical Methods

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