A novel and disposable GP- based impedimetric biosensor using electropolymerization process with PGA for highly sensitive determination of leptin: Early diagnosis of childhood obesity

Özcan, Burcu; Sezgintürk, Mustafa Kemal

doi:10.1016/j.talanta.2020.121985

Cited by 17 publications

(7 citation statements)

References 44 publications

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“…The data showed that the contact angles of all scaffolds were 0° (data not shown), which implies that our scaffolds are more hydrophilic. Abundant functional groups on the surface of PGA and SF, including –OH and –COOH, give them hydrophilicity [ 59 , 60 ]. Simultaneously, the good hydrophobicity indicates our scaffolds will have excellent biocompatibility and provide a moist environment to enhance wound healing.…”

Section: Resultsmentioning

confidence: 99%

Mechanic-Driven Biodegradable Polyglycolic Acid/Silk Fibroin Nanofibrous Scaffolds Containing Deferoxamine Accelerate Diabetic Wound Healing

Zha

Utomo

et al. 2022

Pharmaceutics

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The extracellular matrix (ECM), comprising of hundreds of proteins, mainly collagen, provides physical, mechanical support for various cells and guides cell behavior as an interactive scaffold. However, deposition of ECM, especially collagen content, is seriously impaired in diabetic wounds, which cause inferior mechanical properties of the wound and further delay chronic wound healing. Thus, it is critical to develop ECM/collagen alternatives to remodel the mechanical properties of diabetic wounds and thus accelerate diabetic wound healing. Here, we firstly prepared mechanic-driven biodegradable PGA/SF nanofibrous scaffolds containing DFO for diabetic wound healing. In our study, the results in vitro showed that the PGA/SF-DFO scaffolds had porous three-dimensional nanofibrous structures, excellent mechanical properties, biodegradability, and biocompatibility, which would provide beneficial microenvironments for cell adhesion, growth, and migration as an ECM/collagen alternative. Furthermore, the data in vivo showed PGA/SF-DFO scaffolds can adhere well to the wound and have excellent biodegradability, which is helpful to avoid secondary damage by omitting the removal process of scaffolds. The finite element analysis results showed that the application of silk fibroin-based scaffolds could significantly reduce the maximum stress around the wound. Besides, PGA/SF-DFO scaffolds induced collagen deposition, re-vascularization, recovered impaired mechanical properties up to about 70%, and ultimately accelerated diabetic wound healing within 14 days. Thus, our work provides a promising therapeutic strategy for clinically chronic wound healing.

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Section: Resultsmentioning

confidence: 99%

Mechanic-Driven Biodegradable Polyglycolic Acid/Silk Fibroin Nanofibrous Scaffolds Containing Deferoxamine Accelerate Diabetic Wound Healing

Zha

Utomo

et al. 2022

Pharmaceutics

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“…Equation is the equation for the calculation of the difference in R ct ’s belonging to adiponectin and BSA, where R ct(adiponectin) is the electron-transfer resistance value after binding of antiadiponectin and adiponectin and R ct(BSA) is the electron-transfer resistance after blocking with BSA . The impedance curves and cyclic voltammograms belonging to the increasing adiponectin concentrations are shown in Figure A,B.…”

Section: Resultsmentioning

confidence: 99%

Highly Sensitive and Single-Use Biosensing System Based on a GP Electrode for Analysis of Adiponectin, an Obesity Biomarker

Özcan

Sezgintürk

2021

ACS Biomater. Sci. Eng.

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This study presents a disposable, novel, and sensitive biosensing system to determine adiponectin, an obesity biomarker, in real human serum. The graphite paper (GP) working electrode is a new material for impedimetric biosensors. In the literature, there is no study in which this electrode is used in impedance-based biosensors for adiponectin detection. Sensitive and useful techniques, such as electrochemical impedance spectroscopy and cyclic voltammetry, were utilized for investigation of the modification of the GP electrode surface and optimization and characterization of the constructed biosensor. The single frequency impedance technique was used to study the interactions between antiadiponectin and adiponectin. The morphology of the electrode surface for each immobilization step was examined with scanning electron microscopy. All experimental parameters were optimized to fabricate a rapid and sensitive biosensing system. The designed biosensor presents excellent performance with a wide detection range (0.05–25 pg mL–1) and a low limit of detection (0.0033 pg mL–1) for adiponectin determination. Also, it has been demonstrated that the biosensor sensitively allows for the detection of adiponectin in human serum. The affinity of the designed immunosensor toward other proteins and components was examined in the presence of the target protein (adiponectin), leptin (100 pg mL–1), creatine kinase (50 pg mL–1), parathyroid hormone (50 pg mL–1), and d-glucose (0.5 M). The selectivity of the adiponectin biosensor resulted in high capacity to neglect the interference effect. The constructed biosensor showed good linearity, long-term storage life (10 weeks), high reusability (18 times regenerability), and high ability to detect adiponectin concentrations at picogram levels.

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“…The electrochemical sensor developed by Özcan and Sezgintürk based on graphite paper was particularly interesting [225]. This Please do not adjust margins Please do not adjust margins sensor used anti-leptin as the capture probe and electrochemical impedance spectroscopy to quantify leptin in diluted serum samples.…”

Section: Uterine Cancermentioning

confidence: 99%

“…Leptin, another biomarker, has also had a variety of sensors developed for it, though typically these sensors are initially for diagnosing obesity risk, they none-the-less could be applied to uterine cancer diagnosis. These sensors include electrochemical sensors, [224][225][226][227][228] chemiluminescence, 229,230 and SPR. 231 This wide variety sensors and methods may allow us to bring leptin quantification into diagnosis of uterine cancers.…”

Section: Uterine Cancermentioning

confidence: 99%

Sensor detection in gynaecological medicine

et al. 2022

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A novel and disposable GP- based impedimetric biosensor using electropolymerization process with PGA for highly sensitive determination of leptin: Early diagnosis of childhood obesity

Cited by 17 publications

References 44 publications

Mechanic-Driven Biodegradable Polyglycolic Acid/Silk Fibroin Nanofibrous Scaffolds Containing Deferoxamine Accelerate Diabetic Wound Healing

Mechanic-Driven Biodegradable Polyglycolic Acid/Silk Fibroin Nanofibrous Scaffolds Containing Deferoxamine Accelerate Diabetic Wound Healing

Highly Sensitive and Single-Use Biosensing System Based on a GP Electrode for Analysis of Adiponectin, an Obesity Biomarker

Sensor detection in gynaecological medicine

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