A novel and convenient self-drying system for bacterial preservation

Hays, Henry C.W.; Millner, Paul A.; Jones, Julie; Rayner-Brandes, Michael H.

doi:10.1016/j.mimet.2005.02.014

Cited by 16 publications

(5 citation statements)

References 23 publications

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“…Electrospun microfibers further assembled into non-woven membrane as a sheet of paper format (Scheme 1a). Unlike some traditional methods by which microorganisms were stored as liquid formulation 24 or attached on solid paper, 25 in our strategy microorganisms were encapsulated inside microfibers as a solid formulation, avoiding microorganisms escaping to environment which would cause potential biosafety problems. In addition, the microorganismencapsulated microfibrous membrane (MEM) could be easily detached from the substrate and flexible enough to be packed into any shapes and stored in containers such as test tubes or boxes.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

Encapsulating Microorganisms inside Electrospun Microfibers as a Living Material Enables Room-Temperature Storage of Microorganisms

Han¹,

Liang²,

Cui³

et al. 2018

ACS Appl. Mater. Interfaces

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Room-temperature storage and transportation of microorganisms maximize the power of microorganisms in healthcare, energy, and environment. Recently, paper-based biotechnologies have been developed to enable room-temperature storage of a variety of nonliving biosystems such as diagnostic devices and cell-free systems. Herein, room-temperature storage of living microorganisms is realized by an electrospun nonwoven paper containing convex region, which is composed of coiled microfibers with dense distribution of microorganisms. Microorganisms are encapsulated into the microfibers and remain intact after electrospinning. Poly(ethylene oxide) is used as polymer matrix, and glycerol and dextran are used as additives. When the contents of glycerol and dextran are optimized as 5 and 0.4%, the room-temperature time is prolonged to 2 days, more than 8 folds as compared with the control group. Upon demand, the microorganisms can be activated by adding water and used for culturing microorganisms directly. Furthermore, mechanisms which account for microbial activity and storage are studied. Our microfiber-based strategy is universal for the room-temperature storage of prokaryotic and eukaryotic microorganisms in the solid formulation. Besides, our microorganism/polymer complex structures represent novel living materials via a bottom-up strategy, which are of great potential for new biomedical applications.

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Section: ■ Results and Discussionmentioning

confidence: 99%

Encapsulating Microorganisms inside Electrospun Microfibers as a Living Material Enables Room-Temperature Storage of Microorganisms

Han¹,

Liang²,

Cui³

et al. 2018

ACS Appl. Mater. Interfaces

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“…Lyophilization is the most known and widely applied drying technique but alternative drying methodologies like spraydrying and liquid drying exist (Hays et al 2005;MiyamotoShinohara et al 2008; see also reviews by Morgan et al 2006;Santivarangkna et al 2007). Limited available reports in literature (Green and Woodfor 1992;Safronavo and Nokinova 1996;Smith and Ryan 2008) and our own work on methane-oxidizing bacteria (S. Hoefman, personal communication) suggest that long-term preservation based on drying techniques is less successful for fastidious bacteria.…”

Section: Long-term Preservation Methodsmentioning

confidence: 99%

Safeguarding bacterial resources promotes biotechnological innovation

Heylen

Hoefman

Vekeman

et al. 2012

Appl Microbiol Biotechnol

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“…Activated charcoal (AC) has proven to be very helpful for the removal of several toxic compounds in hemicellulosic hydrolysates used for the cultivation of fungi (Mussatto and Roberto 2004;Chandel et al 2007), and it is used in tissue culture to improve cell growth in plants cultivated in vitro (Teng 1997). AC should also provide proper conditions for the preservation and evaluation of several microbial characteristics in culture (Hays et al 2005).…”

Section: Preservation Of Fungal Materialsmentioning

confidence: 99%

Fungal Cultivation and Production of Polysaccharides

Camelini¹,

Rossi²,

Cardozo³

et al. 2014

Polysaccharides

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Many species of higher basidiomycetes have traditionally been used because of their medicinal properties. The positive effects associated to the consumption of those fungi have been mainly attributed to cell wall polysaccharides, which have important structural roles and are present throughout the entire life cycles of fungi. One of the most consumed and studied species native of the Americas is Agaricus subrufescens, a mushroom prescribed in different countries for prophylaxis and noninvasive treatment of numerous health-related disorders. Prior to the process of extraction, purification, and application of these polysaccharides, one needs to be concerned with the preservation of the specimen and production of fungal biomass. Even though basidiomata (syn. fruiting bodies, mushrooms) generally yield larger volumes of biomass when compared to the mycelium, cultivation of mycelium allows a more efficient control of the process and, therefore, is the method of choice of polysaccharide production. Mycelial biomass can be produced by solid-state fermentation (SSF) or submersed fermentation (SmF). Further separation and concentration of bioactive polysaccharides can be done by means of porous membranes, such as tangential flow nanofiltration.

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A novel and convenient self-drying system for bacterial preservation

Cited by 16 publications

References 23 publications

Encapsulating Microorganisms inside Electrospun Microfibers as a Living Material Enables Room-Temperature Storage of Microorganisms

Encapsulating Microorganisms inside Electrospun Microfibers as a Living Material Enables Room-Temperature Storage of Microorganisms

Safeguarding bacterial resources promotes biotechnological innovation

Fungal Cultivation and Production of Polysaccharides

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