A novel and biocompatible nanofiber of VEGF peptide for enhanced corneal neovascularization suppression

Chen, Zhongxing; Mao, Xin-Jie; Ye, Xiuhong; Li, Siheng; Wu, Tianlu; Wang, Qing; Zhang, Jun; Chen, Lu; Tang, Nana; He, Huanhuan; Wang, Zhen; McAlinden, Colm; Wang, Qinmei; Chen, Shihao; Gao, Rongrong; Huang, Jinhai

doi:10.1016/j.cej.2021.129081

Cited by 9 publications

(9 citation statements)

References 42 publications

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“…Blood vessel formation is induced by many factors, such as hypoxia and inflammation, and followed by the recruitment of inflammatory cells, production of angiogenic factors, basement membrane degradation, and proliferation, migration, and tube formation of vascular endothelial cells, etc. ( Nowak-Sliwinska et al, 2018 ; Chen et al, 2021 ). FK506 liposomes inhibited the specific stages of angiogenesis induced by rhVEGF 165 , including the migration and tube formation of HUVEC-GFP cells.…”

Section: Resultsmentioning

confidence: 99%

“…The microscopic morphological changes of the cornea after the FK506 liposome treatment indicated that liposomes effectively reduced the degree of inflammation and inhibited the formation of CoNV after alkali burns. Furthermore, α-SMA is a vascular smooth muscle marker produced by fibroblasts that can locate mature blood vessels ( Chen et al, 2021 ). After alkali burns, fibroblasts produce α-SMA in the process of scar formation, leading to corneal fibrosis and severe pathological corneal opacity, which greatly affects a patient’s corneal transparency ( Joung et al, 2020 ).…”

Section: Resultsmentioning

confidence: 99%

“…Corneal neovascularization (CoNV), one of the most common causes for reduced corneal transparency and vision loss, can be induced by inflammation and a variety of angiogenic stimuli ( Chuang et al, 2019 ; Chen et al, 2021 ). At present, among the many therapeutic agents used for the treatment of CoNV caused by alkali burns, anti-inflammatory drugs and anti-vascular endothelial growth factor (VEGF) drugs are the most commonly used regimens ( Roshandel et al, 2018 ).…”

Section: Introductionmentioning

confidence: 99%

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Inhibition of Neovascularization and Inflammation in a Mouse Model of Corneal Alkali Burns Using Cationic Liposomal Tacrolimus

Lin

Chen

et al. 2021

Front. Bioeng. Biotechnol.

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Eye drops account for more than 90% of commercialized ophthalmic drugs. However, eye drops have certain shortcomings, such as short precorneal retention time and weak corneal penetration. The requirement of frequent instillation of eye drops also causes poor patient compliance, which may lead to further aggravation of the disease. We aimed to develop a cationic liposome formulation to increase the bioavailability of the therapeutic agent and solve the aforementioned problems. In the present study, we prepared cationic liposomal tacrolimus (FK506) with a surface potential of approximately +30 mV, which could bind to the negatively charged mucin layer of the ocular surface. Our results showed that the content of FK506 in the cornea was increased by 93.77, 120.30, 14.24, and 20.36 times at 5, 30, 60, and 90 min, respectively, in the FK506 liposome group (0.2 mg/ml) compared with the free drug group (0.2 mg/ml). Moreover, FITC-labeled FK506 liposomes significantly prolonged the ocular surface retention time to 50 min after a single dose. In addition, the results of the Cell Counting Kit-8 assay, live and dead cell assay, sodium fluorescein staining, and hematoxylin and eosin staining all indicated that FK506 liposomes had good biological compatibility in both human corneal epithelial cells and mouse eyeballs. Compared with the free drug at the same concentration, FK506 liposomes effectively inhibited vascular endothelial growth factor-induced green fluorescent protein-transduced human umbilical vein endothelial cell migration and tube formation in vitro. In a mouse corneal neovascularization model induced by alkali burns, FK506 liposomes (0.2 mg/ml) enhanced corneal epithelial recovery, inhibited corneal neovascularization, and reduced corneal inflammation, and its therapeutic effect was better than those of the commercial FK506 eye drops (1 mg/ml) and the free drug (0.2 mg/ml). Collectively, these results indicate that cationic FK506 liposomes could increase the efficacy of FK506 in the corneal neovascularization model. Therefore, cationic FK506 liposomes can be considered as a promising ocular drug delivery system.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Inhibition of Neovascularization and Inflammation in a Mouse Model of Corneal Alkali Burns Using Cationic Liposomal Tacrolimus

Lin

Chen

et al. 2021

Front. Bioeng. Biotechnol.

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“…Phage display is to insert the DNA sequence of the exogenous peptide into the phage coat protein gene and express the peptide on the surface of the phage along with the expression of the coat protein. The phage displaying peptide still has protein assembly and infection activity (37)(38)(39). Based on phage display technology, phage libraries were built and used to select targeting phages, such as tumor-targeted peptides, which improved research efficiency and reduced costs.…”

Section: Biopanning the Tumor-targeted Peptidementioning

confidence: 99%

Filamentous Bacteriophage—A Powerful Carrier for Glioma Therapy

et al. 2021

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Glioma is a life-threatening malignant tumor. Resistance to traditional treatments and tumor recurrence present major challenges in treating and managing this disease, consequently, new therapeutic strategies must be developed. Crossing the blood-brain barrier (BBB) is another challenge for most drug vectors and therapy medications. Filamentous bacteriophage can enter the brain across the BBB. Compared to traditional drug vectors, phage-based drugs offer thermodynamic stability, biocompatibility, homogeneity, high carrying capacity, self-assembly, scalability, and low toxicity. Tumor-targeting peptides from phage library and phages displaying targeting peptides are ideal drug delivery agents. This review summarized recent studies on phage-based glioma therapy and shed light on the developing therapeutics phage in the personalized treatment of glioma.

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“…For example, skin repair materials are required to possess tissue regeneration bioactivity, antibacterial effect and anti-inflammatory ability [ [17] , [18] , [19] ]. Angiogenesis is preferred for regeneration of vascularized tissues or organs such as heart and muscle [ 20 ], but should be inhibited during regeneration of avascular tissues such as cartilage and cornea [ 13 , 21 ]. To date, scaffolds including nanoparticles, fibers, hydrogels, microneedles (MNs), sponges and 3D printed scaffolds prepared from polymers, ceramics, metals and composites materials have been extensively investigated for ISTR [ 13 , 16 ].…”

Section: Introductionmentioning

confidence: 99%