A novel amphiphilic copolymer poly(ethylene oxide-co-allyl glycidyl ether)-graft-poly(ε-caprolactone): synthesis, self-assembly, and protein encapsulation behavior

Li, Bin; Gao, Chen; Meng, F.; Li, Taihang; Yue, Jun; Jing, Xiabin; Huang, Yubin

doi:10.1039/c2py20253k

Cited by 33 publications

(34 citation statements)

References 49 publications

(74 reference statements)

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“…However, the CO molecule bound to Hb could be displaced by O 2 if its concentration was high enough. 15,17 As shown in Fig. 6, the met-Hb in ferric state could be transformed into the ferrous CO-Hb in the presence of ascorbic acid under carbon monoxide atmosphere, accompanied with a red shi of the Soret band from 405 nm to 419 nm.…”

Section: Gas-binding Capacitymentioning

confidence: 97%

“…Huang et al synthesized a series of amphiphilic gra copolymers poly-(ethylene oxide-co-allyl glycidyl ether)-g-poly ( (PEAG-g-PCL) which could self-assemble into polymersomes in aqueous solution and encapsulate Hb. 17 While Kumar's group presented another protein nanoparticle consisting of methemoglobin conjugated with polyacrylic acid (PAA) and demonstrated that these conjugates were highly resistant to deactivation by steam sterilization due to the so hydrophilic polymer chains which could facilitate the refolding of the denatured Hb. 18 Dextran, a water soluble polysaccharide, is an optimal molecular model for the synthesis of well-dened polymer derivatives due to its low branching and excellent biocompatibility.…”

Section: Introductionmentioning

confidence: 99%

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Dextran-based thermo-responsive hemoglobin–polymer conjugates with oxygen-carrying capacity

et al. 2014

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The potential toxicity towards human kidneys of hemoglobin (Hb), when used directly, has severely limited its application as a red blood cell substitute and in cancer treatments. In this work, a novel hemoglobinpolymer conjugate was prepared by a reaction between the lysine amino groups of Hb and the carboxyl groups of a copolymer, poly(N-isopropylacrylamide) grafted carboxylated dextran (HOOC-Dex-gPNIPAAm), which was synthesized by single electron transfer living radical polymerization (SET-LRP) andpost-carboxylation. The conjugate was characterized by FTIR, 1 H NMR, SDS-PAGE, DLS, fluorescence spectroscopy and TEM. Results showed it had a relatively low critical micelle concentration (CMC) and could form stable spherical nanoparticles upon heating above the LCST. The redox activity and gasbinding capacity of the Hb conjugate were subsequently confirmed by UV-vis spectroscopy, indicating the retention of Hb bioactivity after conjugation. Furthermore, dextran with different numbers of PNIPAAm chains was synthesized for comparison. It revealed that at 37 C, the temperature above the LCST, the conjugation of Hb to the copolymer Dex-g-PNIPAAm could improve the stability of Hb which increased with the number of PNIPAAm chains.

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Section: Gas-binding Capacitymentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

Dextran-based thermo-responsive hemoglobin–polymer conjugates with oxygen-carrying capacity

et al. 2014

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“…Critical aggregation concentration (CAC) of amphiphilic copolymer was an important parameter to confirm the formation of aggregates. Using pyrene as the hydrophobic probe, the CAC could be calculated from the plot of I 337 /I 333 versus the logarithm of graft copolymer concentrations by fluorescence spectroscopy . As shown in Table , the graft copolymers had relatively low CAC values (≈10 mg · L −1 ), which indicated the graft copolymers could form stable nanoparticles in diluted conditions.…”

Section: Resultsmentioning

confidence: 99%

“…Finally, the dispersion was stored at 4 °C after purging with CO for 30 min. The encapsulation efficiency of Hb was measured indirectly using the cyanide methemoglobin (metHb) method . Meanwhile, the Hb content in the PEH was detected by ICP‐MS.…”

Section: Methodsmentioning

confidence: 99%

Protein-Resistant Biodegradable Amphiphilic Graft Copolymer Vesicles as Protein Carriers

Wang

Yan

et al. 2015

Macromol. Biosci.

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The protein adsorption and self-assembly behavior of biocompatible graft copolymer, poly(lactide-co-diazidomethyl trimethylene carbonate)-g-poly(ethylene glycol) [P(LA-co-DAC)-g-PEG], were systematically studied. The graft copolymers showed enhanced resistance to non-specific protein adsorption compared with their block copolymer counterparts, indicative of the increased effect of PEG density beyond PEG length. Diverse nanostructures including vesicles can be assembled from the amphiphilic graft copolymers with well-defined nano-sizes. Hemoglobin (Hb), as a model protein, can be entrapped in the formed vesicles and keep the gas-binding capacity. The reduced release rate of Hb from graft copolymer vesicles indicated the relatively stable membrane packing compared with block copolymer counterpart.

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“…So far, polymersomes have encapsulated a variety of proteins, including aquaporin Z (Kumar et al, 2007), cytochrome C (CC) (Hvasanov et al, 2011), hemoglobin (Rameez et al, 2008; Li et al, 2012), insulin (Xiong et al, 2007; Christian et al, 2009; Kim H. et al, 2012), immunoglobulin G (Fu et al, 2011), ovalbumin (Stano et al, 2013), and myoglobin (Kishimura et al, 2007). Recent investigations also have found that polymer vesicles with asymmetrical membranes exhibit much higher loading capacity of proteins than symmetrical polymersomes.…”

Section: Applications Of Asymmetric Polymersomesmentioning

confidence: 99%

Asymmetrical Polymer Vesicles for Drug delivery and Other Applications

Zhao

et al. 2017

Front. Pharmacol.

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Scientists have been attracted by polymersomes as versatile drug delivery systems since the last two decades. Polymersomes have the potential to be versatile drug delivery systems because of their tunable membrane formulations, stabilities in vivo, various physicochemical properties, controlled release mechanisms, targeting abilities, and capacities to encapsulate a wide range of drugs and other molecules. Asymmetrical polymersomes are nano- to micro-sized polymeric capsules with asymmetrical membranes, which means, they have different outer and inner coronas so that they can exhibit better endocytosis rate and endosomal escape ability than other polymeric systems with symmetrical membranes. Hence, asymmetrical polymersomes are highly promising as self-assembled nano-delivery systems in the future for in vivo therapeutics delivery and diagnostic imaging applications. In this review, we prepared a summary about recent research progresses of asymmetrical polymersomes in the following aspects: synthesis, preparation, applications in drug delivery and others.

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A novel amphiphilic copolymer poly(ethylene oxide-co-allyl glycidyl ether)-graft-poly(ε-caprolactone): synthesis, self-assembly, and protein encapsulation behavior

Cited by 33 publications

References 49 publications

Dextran-based thermo-responsive hemoglobin–polymer conjugates with oxygen-carrying capacity

Dextran-based thermo-responsive hemoglobin–polymer conjugates with oxygen-carrying capacity

Protein-Resistant Biodegradable Amphiphilic Graft Copolymer Vesicles as Protein Carriers

Asymmetrical Polymer Vesicles for Drug delivery and Other Applications

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