A novel action of highly specific acaricide; bifenazate as a synergist for a GABA-gated chloride channel of Tetranychus urticae [Acari: Tetranychidae]

Hiragaki, Susumu; Kobayashi, Takeru; Ochiai, Noriaki; Toshima, Kayoko; Dekeyser, Mark A.; Matsuda, Kazuhiko; Takeda, Makio

doi:10.1016/j.neuro.2012.01.016

Cited by 24 publications

(16 citation statements)

References 12 publications

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“…Soon after its introduction in the European Union (EU), resistance was reported in T. urticae populations from greenhouse roses in the Netherlands . Surprisingly, bifenazate resistance inherits maternally and investigation of resistance mechanisms led to the discovery of a mitochondrial mode of action, instead of the previously proposed interaction with GABA‐gated chloride channels . Mitochondrial genome sequencing revealed mutations at conserved sites in the mitochondrial cytochrome b gene, suggesting that bifenazate acts as a Q o inhibitor .…”

Section: Discussionmentioning

confidence: 99%

Identification and characterization of new mutations in mitochondrial cytochrome b that confer resistance to bifenazate and acequinocyl in the spider miteTetranychus urticae

Fotoukkiaii

Tan

Xue

et al. 2019

Pest Management Science

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BACKGROUND In spider mites, mutations in the mitochondrial cytochrome b Qo pocket have been reported to confer resistance to the Qo inhibitors bifenazate and acequinocyl. In this study, we surveyed populations of the two‐spotted spider mite Tetranychus urticae for mutations in cytochrome b, linked newly discovered mutations with resistance and assessed potential pleiotropic fitness costs. RESULTS We identified two novel mutations in the Qo site: G132A (equivalent to G143A in fungi resistant to strobilurins) and G126S + A133T (previously reported to cause bifenazate and acequinocyl resistance in Panonychus citri). Two T. urticae strains carrying G132A were highly resistant to bifenazate but not acequinocyl, whereas a strain with G126S + A133T displayed high levels of acequinocyl resistance, but only moderate levels of bifenazate resistance. Bifenazate and acequinocyl resistance were inherited maternally, providing strong evidence for the involvement of these mutations in the resistance phenotype. Near isogenic lines carrying G132A revealed several fitness penalties in T. urticae; a lower net reproductive rate (R0), intrinsic rate of increase (rm) and finite rate of increase (LM); a higher doubling time (DT); and a more male‐biased sex ratio. CONCLUSIONS Several lines of evidence were provided to support the causal role of newly discovered cytochrome b mutations in bifenazate and acequinocyl resistance. Because of the fitness costs associated with the G132A mutation, resistant T. urticae populations might be less competitive in a bifenazate‐free environment, offering opportunities for resistance management. © 2019 Society of Chemical Industry

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Section: Discussionmentioning

confidence: 99%

Identification and characterization of new mutations in mitochondrial cytochrome b that confer resistance to bifenazate and acequinocyl in the spider miteTetranychus urticae

Fotoukkiaii

Tan

Xue

et al. 2019

Pest Management Science

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“…Another reason for the classification 'UN' in IRAC MoA, might be a recent study that revealed that bifenazate has some allosteric modulation effects on T. urticae GABA receptors, albeit in the μM range. Whether these effects correlate with the acaricidal properties has not been documented [74], but resistant lines do not show quantitative expression differences, nor an altered sequence of all GABA receptors [51,75], strongly suggesting this interaction is not responsible for the observed toxicity. In summary, current evidence for a mitochondrial mode of action is quite overwhelming and its use in alternation with compounds such as acequinocyl should be avoided because of the crossresistance issues.…”

Section: Bifenazate Inhibits the Mitochondrial Cytochrome Bc1 Complexmentioning

confidence: 99%

The economic importance of acaricides in the control of phytophagous mites and an update on recent acaricide mode of action research

Leeuwen

Tirry

Yamamoto

et al. 2015

Pesticide Biochemistry and Physiology

273

205

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“…Among them, bifenthrin is one of the non‐systemic pyrethroids . Bifenazate is a novel carbazate acaricide . While cyflumetofen is a novel benzoyl acetonitrile acaricide .…”

Section: Introductionmentioning

confidence: 99%

Laboratory selection, resistance risk assessment, multi‐resistance, and management of Tetranychus urticae Koch to bifenthrin, bifenazate and cyflumetofen on cowpea

Liu

Zhou

Yao

et al. 2020

Pest Management Science

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BACKGROUND Tetranychus urticae (T. urticae) Koch is an important pest of vegetable crops worldwide. In this study, bioassays were carried out to analyze the resistance risk, multi‐resistance and management of T. urticae Koch to bifenthrin, bifenazate and cyflumetofen on cowpea. RESULTS The resistance ratios of the adult T. urticae population to bifenthrin (G16), bifenazate (G12) and cyflumetofen (G12) were 31.29, 9.38 and 5.81, respectively. Realized heritability (h 2) analysis showed that, under a selection pressure of 50–90% mortality, the generations needed to increase 10‐fold LC50 values of bifenthrin, bifenazate and cyflumetofen were 3.64–8.05, 5.75–12.71, and 10.93–24.15, respectively. No obvious multi‐resistance among these three acaricides was observed. Synergist bioassay results showed that microsomal multifunctional oxidase (MFO) was involved in bifenthrin resistance of T. urticae, with a synergistic ratio of 22.38. However, MFO and GSTs were not the main factors conferring the resistance to bifenazate. MFO, glutathione S‐transferases(GSTs), together with esterase contributed to the development of the resistance to cyflumetofen. Additionally, the toxicity selection index test showed that bifenazate was safe to the natural enemy Neoseiulus barkeri (N. barkeri) with a toxicity selection index (TSI) >484.85, while bifenthrin was the least safe (TSI = 0.92). CONCLUSIONS These results demonstrated the T. urticae developed higher resistance risk to bifenthrin compared to bifenazate and cyflumetofen and no obvious multi‐resistance among these three acaricides, providing guidance for designing appropriate strategies for the effective application of bifenthrin, bifenazate and cyflumetofen in the field and delaying the development of insecticide resistance. © 2019 Society of Chemical Industry

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A novel action of highly specific acaricide; bifenazate as a synergist for a GABA-gated chloride channel of Tetranychus urticae [Acari: Tetranychidae]

Cited by 24 publications

References 12 publications

Identification and characterization of new mutations in mitochondrial cytochrome b that confer resistance to bifenazate and acequinocyl in the spider miteTetranychus urticae

Identification and characterization of new mutations in mitochondrial cytochrome b that confer resistance to bifenazate and acequinocyl in the spider miteTetranychus urticae

The economic importance of acaricides in the control of phytophagous mites and an update on recent acaricide mode of action research

Laboratory selection, resistance risk assessment, multi‐resistance, and management of Tetranychus urticae Koch to bifenthrin, bifenazate and cyflumetofen on cowpea

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