A novel 7‑hypoxia‑related long non‑coding RNA signature associated with prognosis and proliferation in melanoma

Abstract

Hypoxia-related long non-coding RNAs (lncRNAs) are important indicators of the poor prognosis of cancers. The present study aimed to explore the potential relationship between melanoma and hypoxia-related lncRNAs. The transcriptome and clinical data of patients with melanoma were downloaded from The Cancer Genome Atlas database. The prognostic hypoxia-related lncRNAs were screened out using Pearson's correlation test and univariate Cox analysis. As a result, a hypoxia-related-lncRNA signature based on the expression of 7 lncRNAs was constructed, with one unfavourable [MIR205 host gene (MIR205HG)] and six favourable (T cell receptor β variable 11-2, HLA-DQB1 antisense RNA 1, AL365361.1, AC004847.1, ubiquitin specific peptidase 30 antisense RNA 1 and AC022706.1) lncRNAs as prognostic factors for melanoma. Patients with melanoma were divided into high- and low-risk groups based on the risk score obtained. Survival analyses were performed to assess the prognostic value of the present risk model. Potential tumour-associated biological pathways associated with the present signature were explored using gene set enrichment analysis. The CIBERSORT algorithm demonstrated the important role of the hypoxia-related lncRNAs in regulating tumour-infiltrating immune cells. Clinical samples collected from our center partly confirmed our findings. Cell Counting Kit-8 and flow cytometry assays indicated the suppression of proliferation of melanoma cells following inhibition of MIR205HG expression. Indicators of the canonical Wnt/β-catenin signalling pathway were detected by western blotting. The present study demonstrated that MIR205HG could promote melanoma cell proliferation partly via the canonical Wnt/β-catenin signalling pathway. These findings indicated a 7-hypoxia-related-lncRNA signature that can serve as a novel predictor of melanoma prognosis.

The promotive role of lncRNA MIR205HG in proliferation, invasion, and migration of melanoma cells via the JMJD2C/ALKBH5 axis

“…It has reported that MIR205HG is upregulated in melanoma [ 13 , 23 ]. The expression pattern of MIR205HG was determined in cultured cell lines and the results showed the upregulation of MIR205HG expression in melanoma cells ( P < 0.01, Fig 1A ).…”

Identification and validation of prognostic features of amino acid metabolism-related lncRNAs in hepatocellular carcinoma

“…In this study, we rst obtained lncRNAs related to amino acid metabolism by co-expression analysis of lncRNAs and amino acid metabolism-related genes, and then used one-way and multi-way Cox regression analysis to obtain a total of LINC02870, AL031985.3, AC011476.3, AC012640.1, AL365361.1 and LUCAT1 and 6 prognosis-related lncRNAs,and prognostic features were constructed.The results of ROC,Kaplan-Meie curve,column line plot and heat map analysis showed that the risk score of the features was an independent predictor for patients with hepatocellular carcinoma and also for patients with different clinical stages,and could reliably predict the prognosis of patients with hepatocellular carcinoma. Among these six amino acid metabolism-related lncRNAs, LINC02870 has been previously reported as hepatocellular carcinoma stem cell and necroptosis-related lncRNA in hepatocellular carcinoma and was signi cantly associated with patient prognosis [32,33] , AC012640.1 can be used to predict the prognosis of patients with alcohol-associated hepatocellular carcinoma [34] , and AL365361.1 is an oncogene that is involved in the prognosis of tumor patients with clear cell renal cell carcinoma, ovarian cancer and melanoma [35][36][37] . AL031985.3 can similarly be involved as a scorch death, iron death and immune-related lncRNA in hepatocellular carcinoma to in uence patient prognosis [38,39] .…”

Identification and validation of prognostic features of amino acid metabolism-related lncRNAs in hepatocellular carcinoma

“…In this study, we rst obtained lncRNAs related to amino acid metabolism by co-expression analysis of lncRNAs and amino acid metabolism-related genes, and then used one-way and multi-way Cox regression analysis to obtain a total of LINC02870, AL031985.3, AC011476.3, AC012640.1, AL365361.1 and LUCAT1 and 6 prognosis-related lncRNAs,and prognostic features were constructed.The results of ROC,Kaplan-Meie curve,column line plot and heat map analysis showed that the risk score of the features was an independent predictor for patients with hepatocellular carcinoma and also for patients with different clinical stages,and could reliably predict the prognosis of patients with hepatocellular carcinoma. Among these six amino acid metabolism-related lncRNAs, LINC02870 has been previously reported as hepatocellular carcinoma stem cell and necroptosis-related lncRNA in hepatocellular carcinoma and was signi cantly associated with patient prognosis [32,33] , AC012640.1 can be used to predict the prognosis of patients with alcohol-associated hepatocellular carcinoma [34] , and AL365361.1 is an oncogene that is involved in the prognosis of tumor patients with clear cell renal cell carcinoma, ovarian cancer and melanoma [35][36][37] . AL031985.3 can similarly be involved as a scorch death, iron death and immune-related lncRNA in hepatocellular carcinoma to in uence patient prognosis [38,39] .…”