A novel 3D indirect co-culture system based on a collagen hydrogel scaffold for enhancing the osteogenesis of stem cells

Kim, Soo Hyun; Han, Shi Huan; Kook, Yun Min; Lee, Kyung Mee; Jin, Yuan; Koh, Won Gun; Lee, Jae Hyup; Lee, Kangwon

doi:10.1039/d0tb01770a

Cited by 24 publications

(24 citation statements)

References 40 publications

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“…The aFPL facilitated even distribution and entrapment of MSCs within the collagen fleece and also served as a source of growth factors and signals for cell migration (Ahmed et al, 2008; Chevalier et al, 2010). A collagen fleece was used as a scaffold hereby, as collagen has been shown to favour cell adhesion and enhance bone formation (Somaiah et al, 2015; Kim et al, 2020). Of note, the improvements reported here appear to be of smaller magnitude than in a previous study utilizing micro‐grafts rich in autologous dental pulp‐derived MSCs versus flap operation by minimally invasive surgical techniques (Ferrarotti et al, 2018).…”

Section: Discussionmentioning

confidence: 99%

A tissue‐engineered biocomplex for periodontal reconstruction. A proof‐of‐principle randomized clinical study

Apatzidou

Bakopoulou

Kouzi-Koliakou³

et al. 2021

J Clinic Periodontology

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Aim: To assess the safety/efficacy of a tissue-engineered biocomplex in periodontal reconstruction.Methods: Twenty-seven intrabony defects were block-randomized across three treatment groups: Group-A (N A = 9) received autologous clinical-grade alveolar bone marrow mesenchymal stem cells (a-BMMSCs), seeded into collagen scaffolds, enriched with autologous fibrin/platelet lysate (aFPL). In Group-B (N B = 10), the collagen scaffold/aFPL devoid of a-BMMSCs filled the osseous defect. Group-C (N C = 8) received Minimal Access Flap surgery retaining the soft tissue wall of defects identically with Groups-A/-B. Subjects were clinically/radiographically assessed before anaesthesia (baseline) and repeatedly over 12 months.Results: Quality controls were satisfied before biocomplex transplantation. There were no adverse healing events. All approaches led to significant clinical improvements (p < .001) with no inter-group differences. At 12 months, the estimated marginal means for all groups were as follows: 3.0 (95% CI: 1.9-4.1) mm for attachment gain; 3.7 (2.7-4.8) mm for probing pocket depth reduction; 0.7 (0.2-1.3) mm increase in recession. An overall greater mean reduction in the radiographic Cemento-Enamel Junction to Bottom Defect (CEJ-BD) distance was found for Groups-A/-C over Group-B (p < .023). Conclusion:Radiographic evidence of bone fill was less pronounced in Group-B, although clinical improvements were similar across groups. All approaches aimed to trigger the innate healing potential of tissues. Cell-based therapy is justified for periodontal reconstruction and remains promising in selected cases.

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Section: Discussionmentioning

confidence: 99%

A tissue‐engineered biocomplex for periodontal reconstruction. A proof‐of‐principle randomized clinical study

Apatzidou

Bakopoulou

Kouzi-Koliakou³

et al. 2021

J Clinic Periodontology

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“…The transplantation of MSC-Matrigel spherical microconstructs into ethanol-injured endometrium promoted tissue repair by increasing blood vessel density, reducing tissue thickness, and restoring gland cells . Furthermore, rat bone marrow derived MSCs encapsulated into calcium alginate microbeads showed enhanced ectopic bone formation in vivo , while the coculture of adipose tissue-derived MSCs with ostoblast encapsulated into alginate-collagen microdrops showed enhanced ectopic bone formation in comparison to MSCs without gel in rats subjected to scalp defects . Alternatively, the implantation of alginate-matrigel drops laden with NSCs into a mouse spinal cord injury models promoted cellular persistence and commitment to a neural lineage within spinal cord slices, in which injury was induced by removing part of the tissue .…”

Section: Applications To Stem Cells and Organoidsmentioning

confidence: 99%

Cell Culture in Microfluidic Droplets

et al. 2022

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Cell manipulation in droplets has emerged as one of the great successes of microfluidic technologies, with the development of single-cell screening. However, the droplet format has also served to go beyond single-cell studies, namely by considering the interactions between different cells or between cells and their physical or chemical environment. These studies pose specific challenges linked to the need for long-term culture of adherent cells or the diverse types of measurements associated with complex biological phenomena. Here we review the emergence of droplet microfluidic methods for culturing cells and studying their interactions. We begin by characterizing the quantitative aspects that determine the ability to encapsulate cells, transport molecules, and provide sufficient nutrients within the droplets. This is followed by an evaluation of the biological constraints such as the control of the biochemical environment and promoting the anchorage of adherent cells. This first part ends with a description of measurement methods that have been developed. The second part of the manuscript focuses on applications of these technologies for cancer studies, immunology, and stem cells while paying special attention to the biological relevance of the cellular assays and providing guidelines on improving this relevance.

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“…The co-culture system also played a corresponding role. A collagen-based hydrogel 3D scaffold experiment demonstrated that the co-cultured ASC and osteoblasts showed enhanced bone formation in the rat skull defect model compared with the ASC single culture group ( Kim et al, 2020 ). Also, the vascularization and bone formation of the osteogenic matrix composed of ASCs and HUVECs were successfully demonstrated through the angiogenesis induced by arteriovenous loop (AVL) surgery in the rat model ( Winkler et al, 2021 ).…”

Section: Distinct Animal Models Of Ascs Contributing To Bone Repair H...mentioning

confidence: 99%

Current applications of adipose-derived mesenchymal stem cells in bone repair and regeneration: A review of cell experiments, animal models, and clinical trials

Zhang

Yang

Cao

et al. 2022

Front. Bioeng. Biotechnol.

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In the field of orthopaedics, bone defects caused by severe trauma, infection, tumor resection, and skeletal abnormalities are very common. However, due to the lengthy and painful process of related surgery, people intend to shorten the recovery period and reduce the risk of rejection; as a result, more attention is being paid to bone regeneration with mesenchymal stromal cells, one of which is the adipose-derived mesenchymal stem cells (ASCs) from adipose tissue. After continuous subculture and cryopreservation, ASCs still have the potential for multidirectional differentiation. They can be implanted in the human body to promote bone repair after induction in vitro, solve the problems of scarce sources and large damage, and are expected to be used in the treatment of bone defects and non-union fractures. However, the diversity of its differentiation lineage and the lack of bone formation potential limit its current applications in bone disease. Here, we concluded the current applications of ASCs in bone repair, especially with the combination and use of physical and biological methods. ASCs alone have been proved to contribute to the repair of bone damage in vivo and in vitro. Attaching to bone scaffolds or adding bioactive molecules can enhance the formation of the bone matrix. Moreover, we further evaluated the efficiency of ASC-committed differentiation in the bone in conditions of cell experiments, animal models, and clinical trials. The results show that ASCs in combination with synthetic bone grafts and biomaterials may affect the regeneration, augmentation, and vascularization of bone defects on bone healing. The specific conclusion of different materials applied with ASCs may vary. It has been confirmed to benefit osteogenesis by regulating osteogenic signaling pathways and gene transduction. Exosomes secreted by ASCs also play an important role in osteogenesis. This review will illustrate the understanding of scientists and clinicians of the enormous promise of ASCs’ current applications and future development in bone repair and regeneration, and provide an incentive for superior employment of such strategies.

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A novel 3D indirect co-culture system based on a collagen hydrogel scaffold for enhancing the osteogenesis of stem cells

Abstract: In this study, the paracrine effect between adipose-derived mesenchymal stem cells (ADSCs) and osteoblasts was investigated in collagen-based three-dimensional (3D) scaffolds.

Cited by 24 publications

References 40 publications

A tissue‐engineered biocomplex for periodontal reconstruction. A proof‐of‐principle randomized clinical study

A tissue‐engineered biocomplex for periodontal reconstruction. A proof‐of‐principle randomized clinical study

Cell Culture in Microfluidic Droplets

Current applications of adipose-derived mesenchymal stem cells in bone repair and regeneration: A review of cell experiments, animal models, and clinical trials

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