A novel 3D cell culture model to study the human small intestinal immune landscape

Schimpel, Christa; Passegger, Christina; Egger, Simone; Tam‐Amersdorfer, Carmen; Strobl, Herbert

doi:10.1002/eji.202250131

Cited by 3 publications

(2 citation statements)

References 86 publications

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“…One of the possible strategies to overcome these gaps is through the development of advanced in vitro models, considered more representative of the NM interactions and effects in vivo. Especially for inhalation and ingestion toxicology and bio-interaction purposes, huge efforts are being made to standardize the use of 3D reconstructed tissues, incorporating immune cells and including the use of physiologically relevant fluids [ 133 , 134 ]. In parallel with the enhancement of the in vitro systems, the use of non-mammal vertebrate organisms has been taken into consideration as valid alternative models for NM screening [ 135 ].…”

Section: Xenopus In Emerging Areas Of Biosciencementioning

confidence: 99%

Xenopus laevis (Daudin, 1802) as a Model Organism for Bioscience: A Historic Review and Perspective

Carotenuto

Pallotta

Tussellino

et al. 2023

Biology

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In vitro systems have been mainly promoted by authorities to sustain research by following the 3Rs principle, but continuously increasing amounts of evidence point out that in vivo experimentation is also of extreme relevance. Xenopus laevis, an anuran amphibian, is a significant model organism in the study of evolutionary developmental biology, toxicology, ethology, neurobiology, endocrinology, immunology and tumor biology; thanks to the recent development of genome editing, it has also acquired a relevant position in the field of genetics. For these reasons, X. laevis appears to be a powerful and alternative model to the zebrafish for environmental and biomedical studies. Its life cycle, as well as the possibility to obtain gametes from adults during the whole year and embryos by in vitro fertilization, allows experimental studies of several biological endpoints, such as gametogenesis, embryogenesis, larval growth, metamorphosis and, of course, the young and adult stages. Moreover, with respect to alternative invertebrate and even vertebrate animal models, the X. laevis genome displays a higher degree of similarity with that of mammals. Here, we have reviewed the main available literature on the use of X. laevis in the biosciences and, inspired by Feymann’s revised view, “Plenty of room for biology at the bottom,” suggest that X. laevis is a very useful model for all possible studies.

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Section: Xenopus In Emerging Areas Of Biosciencementioning

confidence: 99%

Xenopus laevis (Daudin, 1802) as a Model Organism for Bioscience: A Historic Review and Perspective

Carotenuto

Pallotta

Tussellino

et al. 2023

Biology

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“…The classic approach is cultivation using Transwell technology. Studies using Transwell inserts are very popular due to their simplicity (Schimpel et al, 2023). However, this approach has several significant methodological limitations.…”

Section: Introductionmentioning

confidence: 99%

The effect of human PBMCs immobilization on their Аβ42 aggregates-dependent proinflammatory state on a cellular model of Alzheimer’s disease

Kot,

Kurbanov

et al. 2024

Front. Neurosci.

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The leading pathological mechanisms of Alzheimer’s disease are amyloidosis and inflammation. The presented work was aimed to study the effect of human peripheral blood mononuclear cells (hPBMcs) cells-matrix adhesion on their pro-inflammatory state in vitro. Although direct interaction of Аβ42 to PBMC is not a cellular model of Alzheimer’s disease, PBMCs may serve as test cells to detect Аβ42-dependent molecular effects in monitoring disease progression. Peripheral blood mononuclear cells (PBMCs) are used to assess changes in cytokines released in response to diseases or Alzheimer’s disease-specific cytotoxic molecules such as Aβ42. The effect of recombinant amyloid β-peptide rАβ42 on the concentration of endogenous amyloid β-peptide Aβ40 and pro-inflammatory cytokines TNFα and IL-1β in human peripheral blood mononuclear cells that were cultured in suspension and immobilized in alginate microcarriers for 24 h were investigated. The localization and accumulation of Aβ40 and rAβ42 peptides in cells, as well as quantitative determination of the concentration of Aβ40 peptide, TNFα and IL-1β cytokines, was performed by intravital fluorescence imaging. The results were qualitatively similar for both cell models. It was determined that the content of TNFα and Aβ40 in the absence of rAβ42 in the incubation medium did not change for 24 h after incubation, and the content of IL-1β was lower compared to the cells that were not incubated. Incubation of cells in vitro with exogenous rAβ42 led to an increase in the intracellular content of TNFα and Aβ40, and no accumulation of IL-1β in cells was observed. The accumulation of Aβ40 in the cytoplasm was accompanied by the aggregation of rAβ42 on the outer surface of the cell plasma membrane. It was shown that the basic levels of indicators and the intensity of the response of immobilized cells to an exogenous stimulus were significantly greater than those of cells in suspension. To explore whether non-neuronal cells effects in alginate microcarriers were cell-matrix adhesion mediated, we tested the effect of blocking β1 integrins on proamyloidogenic and proinflammation cellular state. Immobilization within alginate hydrogels after incubation with the β1 integrins blocking antibodies showed a remarkable inhibition of TNFα and Aβ40 accumulation in rAβ42-treated cells. It can be concluded that activation of signal transduction and synthesizing activity of a portion of mononuclear cells of human peripheral blood is possible (can significantly increase) in the presence of cell-matrix adhesion.

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In Vitro Models for Investigating Intestinal Host–Pathogen Interactions

McCoy,

Oldroyd,

Yang

et al. 2023

Advanced Science

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Infectious diseases are increasingly recognized as a major threat worldwide due to the rise of antimicrobial resistance and the emergence of novel pathogens. In vitro models that can adequately mimic in vivo gastrointestinal physiology are in high demand to elucidate mechanisms behind pathogen infectivity, and to aid the design of effective preventive and therapeutic interventions. There exists a trade‐off between simple and high throughput models and those that are more complex and physiologically relevant. The complexity of the model used shall be guided by the biological question to be addressed. This review provides an overview of the structure and function of the intestine and the models that are developed to emulate this. Conventional models are discussed in addition to emerging models which employ engineering principles to equip them with necessary advanced monitoring capabilities for intestinal host‐pathogen interrogation. Limitations of current models and future perspectives on the field are presented.

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A novel 3D cell culture model to study the human small intestinal immune landscape

Cited by 3 publications

References 86 publications

Xenopus laevis (Daudin, 1802) as a Model Organism for Bioscience: A Historic Review and Perspective

Xenopus laevis (Daudin, 1802) as a Model Organism for Bioscience: A Historic Review and Perspective

The effect of human PBMCs immobilization on their Аβ42 aggregates-dependent proinflammatory state on a cellular model of Alzheimer’s disease

In Vitro Models for Investigating Intestinal Host–Pathogen Interactions

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