A note on the determination of the ester sulphate content of sulphated polysaccharides

Dodgson, K. S.; Price, Robert G.

doi:10.1042/bj0840106

Cited by 1,440 publications

(483 citation statements)

References 11 publications

(13 reference statements)

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“…Sulfation level was determined by using the BaCl 2 -gelatin turbidimetry method (Dodgson and Price 1962). The content of crude protein was determined by Lowry method (Lowry, Rosebrough et al 1951).…”

Section: Chemical Analysis Of Seaweed Polysaccharidesmentioning

confidence: 99%

“…General composition analysis was carried out using methods described by other research groups (Lowry, Rosebrough et al 1951;DuBois, Gilles et al 1956;Dodgson and Price 1962) and the results were in Depending on their major monosaccharide composition, different sugars were used as standards when running total sugar content determination to reduce the individual error.…”

Section: Extraction and General Analysis Of Algal Polysaccharidesmentioning

confidence: 99%

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Properties of polysaccharides in several seaweeds from Atlantic Canada and their potential anti-influenza viral activities

Jiao

Wang

et al. 2012

J. Ocean Univ. China

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Objective: To explore the polysaccharides from selected seaweeds of Atlantic Canada and to evaluate their potential anti-influenza virus activities. Methods: Polysaccharides were isolated from several Atlantic Canadian seaweeds, including three red algae (Polysiphonia lanosa, Furcellaria lumbricalis, and Palmaria palmata), two brown algae (Ascophyllum nodosum and Fucus vesiculosus), and one green alga (Ulva lactuca) by sequential extraction with cold water, hot water, and different alkali solutions. These polysaccharides were analyzed for monosaccharide composition and other general  First author: Guangling Jiao, Ph.D candidate, Glycochemistry and glycobiology, Email: sebrina2006@gmail.com;  Corresponding author: Guangli Yu, Professor, Glycochemistry and glycobiology, E-Mail: glyu@ouc.edu.cn. chemical properties, and they were evaluated for anti-influenza virus activities. Results:Total sugar contents in these polysaccharides ranged from 15.4 % (in U. lactuca) to 91.4 % (in F. lumbricalis); sulfation level was as high as 17.6 % in a polysaccharide from U. lactuca whereas it couldn't be detected in an alikali-extract from P. palmaria. For polysaccharides extracted from red seaweeds, the main polysaccharides were sulfated agar and carrageenan for P. lanosa, F. lumbricalis, and xylans for P. palmata. In brown seaweeds, the polysaccharides mainly contained sulfated fucans, whereas the polysaccharides in green seaweeds were composed of heteroglycuronans. Screening for antiviral activity against influenza A/PR/8/34 (H1N1) virus revealed that brown algal polysaccharides were particularly effective. Conclusion: Seaweeds from Atlantic Canada are a good source of marine polysaccharides with potential antiviral properties.

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Section: Chemical Analysis Of Seaweed Polysaccharidesmentioning

confidence: 99%

Section: Extraction and General Analysis Of Algal Polysaccharidesmentioning

confidence: 99%

Properties of polysaccharides in several seaweeds from Atlantic Canada and their potential anti-influenza viral activities

Jiao

Wang

et al. 2012

J. Ocean Univ. China

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“…O conteúdo de proteínas contaminantes (PC) foi estimado pelo método descrito por BRADFORD (1976), usando albumina sérica bovina (Sigma-Aldrich, St. Louis, USA) como padrão. A determinação do conteúdo de sulfato livre (SL) foi estimada de acordo com DODGSON & PRICE (1962), utilizando sulfato de sódio para a obtenção da curva-padrão.…”

Section: Composição Químicaunclassified

Isolamento, fracionamento e atividade anticoagulante de iota-carragenanas da Solieria filiformis

et al. 2010

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RESUMO Este estudo teve como objetivo isolar, fracionar e avaliar o potencial anticoagulante de iota-carragenanas (iCARs) da rodofícea Solieria filiformis, quando obtidas por dois métodos de extração (M I e M II). As i-CARs foram isoladas ABSTRACT This study aimed to isolate, fractionate and evaluate the anticoagulant potential of iota-carrageenans (i-CARs) from Solieria filiformis when two extraction methods (M I and M II) were used. i-CARs were isolated with papain in 0.1M sodium acetate (pH 5.0) containing 5mM cystein and 5mM EDTA (M I) or water (80°C) (M II), and then their chemical composition of total carbohydrates, free sulfate (FS) and contaminant proteins were determined. i-CARs were submitted to anionexchange chromatography (DEAE-cellulose) using a sodium chloride gradient,being evaluated the activated partial thromboplastin time (APTT) and prothrombin time of obtained fractions and compared to heparin (

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“…Sulphate was determined by the turbidimetric method of Dodgson and Price [19] as modified by Starkey et al [1].…”

Section: Preparation Of Gastric Mucosa Homogenate Fractions Wistar Rmentioning

confidence: 99%

Isolation and partial characterization of rat gastric mucous glycoprotein

Spee-Brand

Strous

Kramer

1980

Biochimica et Biophysica Acta (BBA) - Protein Structure

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SummaryMucus glycoproteins from the rat stomach were characterized after their isolation from homogenates of the superficial gastric mucosa by equilibrium centrifugation in CsC1 density gradients. Water-soluble as well as water-insoluble glycoproteins were studied. The latter were solubilized by 2-mercaptoethanol reduction of the homogenate. From both homogenate fractions the same two glycoproteins 1 and 2 were purified, glycoprotein 1 being present in considerably higher amount than glycoprotein 2. Their respective buoyant densities in a CsC1 gradient were 1.47--1.50 g/ml and 1.56--1.58 g/ml. The two glycoproteins expressed slight differences in gel electrophoresis and gel filtration. The results from column chromatographic comparisons between reduced and unreduced glycoproteins indicated strongly that both glycoproteins 1 and 2 were built from subunits kept together by S-S bonds. The s20,w values of the reduced glycoproteins 1 and 2 were 15.7 S and 11.6 S. Glycoprotein 1 contained 5% protein, 70% carbohydrate and 1--2% sulphate, whereas these percentages for glycoprotein 2 were 10% protein, 65% carbohydrate and 10% sulphate. The molar proportions of the main sugar components galactose, fucose, glucosamine and galactosamine were 4 : 2 : 4 : 1 (glycoprotein 1) and 3 : 2 : 3 : 1 (glycoprotein 2). Blood-group activity A was expressed by glycoprotein 1, whereas glycoprotein 2 showed mainly blood-group activity Le b, some B activity and also some A activity, but to a lesser extent than glycoprotein 1.

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A note on the determination of the ester sulphate content of sulphated polysaccharides

Cited by 1,440 publications

References 11 publications

Properties of polysaccharides in several seaweeds from Atlantic Canada and their potential anti-influenza viral activities

Properties of polysaccharides in several seaweeds from Atlantic Canada and their potential anti-influenza viral activities

Isolamento, fracionamento e atividade anticoagulante de iota-carragenanas da Solieria filiformis

Isolation and partial characterization of rat gastric mucous glycoprotein

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