A note on a counterexample of Delgado

Brooks, Jennifer

doi:10.4310/jdg/1214509284

Cited by 3 publications

(4 citation statements)

References 2 publications

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“…The data of the present paper suggest that enzymes may be bound to a variety of cellular structures including plasma membranes, mitochondrial membranes and F-actin (low speed pellet) and endoplasmic reticular membranes and tubulin (high speed .pellet). A large percentage of enzyme activity may also be free (high speed superda'tant) or bound in soluble enzyme complexes (24). These results show that a single type of multi-enzYme complex localized to a specific subcellutar structure does not exist in hepatopancreas cells.…”

Section: Biochemistryond Molecular Biology Internationalmentioning

confidence: 80%

“…It is important to know the subcellular distribution of glycolytic enzymes when evaluating metabolic models that relate changes in enzyme binding to changes in enzyme activity. These models propose the existence of large multi-enzyme complexes that may be bound to subcellular structures such as F-actin (2,3) or microtubules (14), or may exist freely in solution (see 24). Initial experiments on exercised and anoxic muscle tissue showed that particulate-bound enzymes apparently responded to changes in glycolytic flux (3,5).…”

Section: Discussionmentioning

confidence: 99%

“…For example, we have calculated tha1~ binding of PFK (only) to F-actin filaments would result in a 31 percent increase in its activity. This increase would result from allosteric activation by F-actin and from a de-inhibition of the soluble enzyme (24). Binding of enzymes to other subcellular structure may also play an important role in regulating enzyme activity, although initial experiments suggest that this may be primarily inhibitory rather than stimulatory (26).…”

Section: Biochemistryond Molecular Biology Internationalmentioning

confidence: 99%

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Glycolytic enzyme binding in Otala lactea hepatopancreas: Effect of taxol, colchicine and cytochalasin B and D on the in vivo enzyme distribution

Brooks

Storey

1997

IUBMB Life

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The effect of cytoskeleton modulators on glycolytic enzyme binding was examined in the hepatopancreas of Otala lactea in an attempt to identify potential cellular binding sites. Binding was followed by measuring phosphofructokinase (PFK), aldolase (ALD), glyceraldehyde 3-phosphate dehydrogenase (GAPDH) and pyruvate kinase (PK) distribution between low speed pellets (12,000xg), high speed pellets (lO0,O00xg) and high speed supernatants.Taxol (which stabilizes microtubules), colchicine (which destabilizes microtubules) and cytochalasin B and D (which destabilize F-actin filaments) were added to the homogenate prior to centrifugation. Addition of taxol increased the amount of PFK associated with the high speed pellet. Cytochalasin B and D reduced the binding of PFK and PK to the low speed pellet. ALD and GAPDH binding were unaffected by any treatment. Lowering the pH of the crude homogenate increased PFK binding to the low speed pellet by 33%. This effect could be reversed by addition of cytochalasin B and D suggesting that pH influences the PFK-F-actin interaction in viva. The differential binding response of PFK, PK, ALD and GAPDH to added effectors suggests that, in the cell, PFK and PK are bound to different subcellular structural elements than are ALD and GAPDH.

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Section: Biochemistryond Molecular Biology Internationalmentioning

confidence: 80%

Section: Discussionmentioning

confidence: 99%

Section: Biochemistryond Molecular Biology Internationalmentioning

confidence: 99%

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Glycolytic enzyme binding in Otala lactea hepatopancreas: Effect of taxol, colchicine and cytochalasin B and D on the in vivo enzyme distribution

Brooks

Storey

1997

IUBMB Life

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“…By contrast, Shiraki and collelagues (2) found that solubilization of rat muscle AMPD increased steadily with increasing exogenous KC1 up to 500 raM. A similar increase in the amount of soluble enzyme (decrease in bound enzyme) with increasing salt concentration has been noted for several glycolytic enzymes and fructose-l,6-bisphosphatase in extracts from fish muscle (1,(11)(12)(13)(14)(15). For these enzymes it was determined that electrostatic forces played a critical role in their binding with cellular structures and that binding was disrupted by Vol.…”

Section: Vol 43 No 3 1997mentioning

confidence: 84%

Unusual AMP‐deaminase solubilization from teleost fish white muscle

1997

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A previous study described an unusual influence of neutral salts on the behavior of trout muscle AMP‐deaminase (AMPD) in its interactions with subcellular particulate matter (Lushchak and Storey 1994, Fish Physiol. Biochem. 13: 356‐368). The present study shows that this behavior is also shared by the muscle enzyme of two other fish species, sea scorpion (Scorpaena porcus) and corb (Sciena umbra), indicating that this describes a principle for AMPD interaction with cellular particulate material. AMPD binding to particulate matter increased with increasing KC1 concentration through the physiological range (100‐200 mM), but at higher salt concentrations the amount of bound enzyme was reduced. The pattern of binding was not influenced by hydrophobic interactions since addition of the nonionic detergents, Triton X‐100 or Tween‐80, did not alter the distribution of bound versus free enzyme although both detergents, at low concentrations, enhanced enzyme maximal activity. AMPD binding to particulate matter was also influenced by pH, the amount of free enzyme rising by nearly 3‐fold as pH fell within the physiological range from 7.5 to 6.5. It is concluded that neither electrostatic nor hydrophobic forrces alone can account for the unusual solubilization of AMPD from fish muscle and it is possible that the effect is also related to ion‐induced conforlnational changes in the structure of AMPD and/or of the myosin to which the enzyme binds.

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International Competitiveness, Tax Incentives, and a New Argument for Tax Sparing: Preventing Double Taxation by Crediting Implicit Taxes

Knoll

2008

SSRN Journal

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A note on a counterexample of Delgado

Cited by 3 publications

References 2 publications

Glycolytic enzyme binding in Otala lactea hepatopancreas: Effect of taxol, colchicine and cytochalasin B and D on the in vivo enzyme distribution

Glycolytic enzyme binding in Otala lactea hepatopancreas: Effect of taxol, colchicine and cytochalasin B and D on the in vivo enzyme distribution

Unusual AMP‐deaminase solubilization from teleost fish white muscle

International Competitiveness, Tax Incentives, and a New Argument for Tax Sparing: Preventing Double Taxation by Crediting Implicit Taxes

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