A nosocomial outbreak of Candida parapsilosis in southern Sweden verified by genotyping

Abstract

In a haematology ward, Candida parapsilosis was found in blood cultures from 4 patients within a month. As C. parapsilosis is known to have a restricted genetic diversity, a combined methodological approach was adopted to establish a possible epidemiological relationship among the isolates (n = 9). Multilocus sequence typing and random amplified polymorphic DNA analysis suggested a clonal origin of the isolates. The clonal origin was confirmed by microsatellite analysis, a method that displayed the highest discriminatory level and readily differentiated cluster isolates from 2 epidemiologically unrelated strains of C. parapsilosis. The use of novel methods of genotyping such as microsatellite analysis will facilitate epidemiological investigations of potential clonal outbreaks of fungaemia.

Investigation of a Cluster ofCandida albicansInvasive Candidiasis in a Neonatal Intensive Care Unit by Pulsed-Field Gel Electrophoresis

“…Various molecular typing methods are now available that allow understanding of the Candida spp. transmission, colonization and infection in neonatal care setting [5, 9–12]. It has long been assumed that the vertical transmission of the Candida species, mainly C. albicans , is an important mode of acquisition of the yeasts by neonates at delivery [4].…”

“…They include electrophoretic karyotyping, southern blot hybridization, restriction fragment length polymorphism (RFLP) analysis, randomly amplified polymorphic DNA (RAPD) analysis, PCR-based fingerprinting, and multilocus sequence typing [5, 9–12]. …”

Prevalence of biofilm formation in clinical isolates of Candida species causing bloodstream infection

“…The assay was carried out at least two times independently with two technical replicates each time. In each experiment, C. parapsilosis and C. parapsilosis SMI 828 ( 22 unpublished results) were included as a negative and positive control for biofilm formation respectively.…”

Genotyping of Candida parapsilosis from three neonatal intensive care units (NICUs) using a panel of five multilocus microsatellite markers: Broad genetic diversity and a cluster of related strains in one NICU

“…Efforts to apply multilocus sequence typing to CP isolates, however, were hindered because CP is diploid or aneuploid and possesses little (60.1%) inter-strain DNA sequence variation (Fundyga et al, 2004;Odds and Jacobsen, 2008;van Asbeck et al, 2009b). Instead, the trend in CP genotyping is towards reliance on length variations associated with microsatellites (Brillowska-Dabrowska et al, 2009;Lasker et al, 2006;Sabino et al, 2010).…”