A Nonproducer T Lymphoblastoid Cell Line from Marek's Disease Transplantable Tumor (JMV)

Nazerian, K.; Stephens, Elizabeth; Sharma, J. M.; Lee, L F; Gailitis, M; Witter, R. L.

doi:10.2307/1589365

Cited by 103 publications

(55 citation statements)

References 7 publications

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“…Ten chicken cell lines established from chicken lymphoma were used in this study, MD cell lines: MSB1 [1], MTB1 [9], JP1, JP2 [31], HP1, HP2 [20] and RP1 [18]; AL cell lines: 1104B1 [6] and CU10 [3] an RE cell line: Ku7 [22]. All cell lines were grown at 40°C in RPMI1640 medium supplemented with 10% fetal bovine serum, penicillin G (200 U/ml), streptomycin (200 mg/ml) and 2 × 10 5 M 2-mercaptoethanol.…”

Section: Cell Lines and Tumor Samplesmentioning

confidence: 99%

Analysis of Tumor Suppressor Gene p53 in Chicken Lymphoblastoid Tumor Cell Lines and field Tumors.

Takagi

Ohashi

Morimura

et al. 1998

The Journal of Veterinary Medical Science

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ABSTRACT. To determine whether there is any abnormalities of the p53 gene in chicken lymphoblastoid tumor cell lines derived from Marek's disease (MD), lymphoid leukosis, reticuloendotheliosis, and field tumors, some portions of p53 cDNA corresponding to core and C-terminal domains (nucleotide positions 277-1104 in the p53 open reading frame (ORF)) were sequenced. Several mutations were identified in both cell lines and field tumors. However, none of these mutations is localized at the "hot spot", which has been reported as the site for transformation-activating mutations. Moreover, partial cDNA clones with a 122-bp deletion in the p53 ORF were identified in two cell lines, MSB1 and MTB1 derived from MD tumors. Southern blot analysis showed that no deletion occurred in the genome of p53 in MSB1, indicating that deletion occurred at the transcriptional level. This deletion could cause a frame shift of the encoding p53 protein, possibly resulting in the generation of a functionally different p53 protein. However, we confirmed that p53 mRNA without deletion is also present in each of these cell lines. These mutations of the p53 gene and deletion in the p53 transcript may be ones of molecular changes specific to the transformation induced by MD virus. -KEY WORDS: Marek's disease, p53, RT-PCR, transformation.J. Vet. Med. Sci. 60(8): 923-929, 1998 demyelination of peripheral nerves [23]. The molecular mechanism of transformation by MDV has not been fully understood. Unlike retroviruses, MDV does not have any apparent oncogenes, suggesting that interaction of viral proteins with cellular oncoproteins or activation of cellular oncogenes are important for the transformation mechanism. Recently, it has been reported that Meq, which has a homology with members of the jun/fos oncoprotein family, could be a viral oncogene based on its potential role as a transcription factor [30]. In addition, the Meq protein has been shown to have a potential to interact with p53 [2], suggesting possible involvement of this interaction in the transformation process by MDV. However, no information is available concerning the changes in the p53 gene of host cells related to transformation by MDV. Thus, to determine whether p53 mutations are involved in the neoplastic process of chicken lymphoma, we analyzed the molecular structure of chicken p53 genes and its transcripts in chicken tumor cell lines derived from MD, avian leukosis (AL), reticuloendotheliosis (RE), and field tumors. We here reported that tumor cell lines possess mis-sense mutations and deletion in the p53 transcript. MATERIALS AND METHODS Cell

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Section: Cell Lines and Tumor Samplesmentioning

confidence: 99%

Analysis of Tumor Suppressor Gene p53 in Chicken Lymphoblastoid Tumor Cell Lines and field Tumors.

Takagi

Ohashi

Morimura

et al. 1998

The Journal of Veterinary Medical Science

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“…DT95 and DT40 are c-myc-transformed chicken B-cell lines (Baba et al, 1985). MSB-1 and RP-1 are T-cell lines derived from lymphomas induced by Marek's disease virus (Akiyama and Kato, 1974;Nazerian et al, 1977). BM2 is a macrophage-like cell line derived from avian myeloblastosis virus (AMV) transformed yolk sac cells (Moscovici et al, 1982).…”

Section: Methodsmentioning

confidence: 99%

The activation of TC10, a Rho small GTPase, contributes to v-Rel-mediated transformation

et al. 2006

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“…This highly malignant tumour kills chicks in 5 to 8 days, much less time than is required for induction of tumours by inoculation of the virus or virus-producer cell lines such as MSB 1. The RP 1 and BP 1 cell lines are derived from this JMV tumour, and have a T-ceU marker and MATSA, but lack any MDV rescuable in vivo or in vitro (Nazerian et al, 1977;Sekiya et al, 1977). On the other hand, the MSB1 and HP1 line cells, which are derived from MD lymphomas, also have a T-cell marker and MATSA, but produce MDV.…”

Section: Number Of Viable Cells In Control -Number Of Viable Cells Inmentioning

confidence: 99%

“…However, the exact nature of MATSA is not clearly understood. Dissimilarity or partial qualitative differences of MATSA in different MD cell lines have been noted (Witter et al, 1975;Sharma et al, 1977;Nazerian et al, 1977;Sugimoto et al, 1979;Rennie & Powell, 1979). Recently we demonstrated the presence of Hanganutziu and Deicher (H-D)-like heterophile antibody in chicken anti-MATSA sera (Ikuta et aL, 1980).…”

mentioning

confidence: 99%

“…The MDV-transformed cell lines used were MDCC-MSB 1 (Akiyama & Kato, 1974) and MDCC-HP1 (Powell et al, 1974), which were derived from MD lymphomas, and MDCC-RP1 (Nazerian et al, 1977) and MDCC-BP1 (Sekiya et al, 1977), which were derived from the transplantable MD tumour, JMV (Sevoian et al, 1964). The ALV- 1-4)Glc-ceramide] used were described previously (Naiki, 1971;Naiki et aL, 1972).…”

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Expression of Forssman Antigen of Avian Lymphoblastoid Cell Lines Transformed by Marek's Disease Virus or Avian Leukosis Virus

Ikuta

Kitamoto

Shoji

et al. 1981

Journal of General Virology

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SUMMARYThe expression of Forssman-type heterophile antigen on Marek's disease (MD) virus (MDV)-transformed cell lines, MDCC-MSB 1, -HP 1, -RP 1 and -BP 1, and avian leukosis virus (ALV)-transformed cell lines, LSCC-1104B1 and -1104X5 was investigated by membrane immunofluorescence and complement-dependent antibody cytotoxicity tests. Forssman antigen was detected on a high percentage of the cells in two ALV-transformed cell lines and on a smaller percentage of splenic lymphocytes from normal chicken. Of the MDV-transformed cell lines tested only the RP1 and BP1 cell lines, derived from transplantable MD tumours, expressed Forssman antigen, while the MSB 1 and HP 1 cell lines, derived from MD lymphomas, did not. Forssman antigen appears to be unrelated to MD tumour-associated surface antigen (MATSA).

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A Nonproducer T Lymphoblastoid Cell Line from Marek's Disease Transplantable Tumor (JMV)

Cited by 103 publications

References 7 publications

Analysis of Tumor Suppressor Gene p53 in Chicken Lymphoblastoid Tumor Cell Lines and field Tumors.

Analysis of Tumor Suppressor Gene p53 in Chicken Lymphoblastoid Tumor Cell Lines and field Tumors.

The activation of TC10, a Rho small GTPase, contributes to v-Rel-mediated transformation

Expression of Forssman Antigen of Avian Lymphoblastoid Cell Lines Transformed by Marek's Disease Virus or Avian Leukosis Virus

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