A Noninvasive Test for MicroRNA Expression in Oral Squamous Cell Carcinoma

Abstract

MicroRNAs have recently been proposed as non-invasive biomarkers in Oral Squamous Cell Carcinoma (OSCC). The aim of this study was to analyze the expression of a panel of miRNAs in epithelial cells collected by oral brushing from OSCCs from regenerative areas after OSCC surgical resection and from their respective normal distant mucosa. Oral brushing specimens were collected from 24 healthy donors, 14 OSCC patients with specimens from tumour and normal distant mucosa, and from 13 patients who had OSCC resection, with samples from regenerative areas after OSCC resection and normal distant mucosa. Expression levels of eight targets (miR-21, miR-375, miR-345, miR-181b, miR-146a, miR-649, miR-518b, and miR-191) were evaluated by real-time Polymerase Chain Reaction (PCR). A highly significant between-group difference was found for miR-21 (F = 6.58, p < 0.001), miR-146a (F = 6.974, p < 0.001), and miR-191 (F = 17.07, p < 0.001). The major difference was observed between samples from healthy donors and from OSCC brushing, whereas no significant differences were observed between areas infiltrated by OSCC and their respective normal distant mucosa. Furthermore, altered expression of miR-146a and miR-191 was also observed in regenerative areas after OSCC resection. Conclusions: Oral brushing could be proposed as a noninvasive method to study microRNA expression in oral mucosa in OSCC patients.

MicroRNA expression patterns in oral squamous cell carcinoma: hsa‐mir‐99b‐3p and hsa‐mir‐100‐5p as novel prognostic markers for oral cancer

“…[19][20][21][47][48][49][50][51][52][53] In cell culture studies, overexpression of hsa-mir-21-5p is associated with increased proliferation, migration, invasion, and colony formation as well as an decreased rate of apoptosis by targeting clusterin or PTEN. Numerous studies demonstrated an overexpression of hsa-mir-21-5p in OSCC/HNSCC tissue, blood samples, oral cytology samples, and cell lines.…”

“…Numerous studies demonstrated an overexpression of hsa-mir-21-5p in OSCC/HNSCC tissue, blood samples, oral cytology samples, and cell lines. [19][20][21][47][48][49][50][51][52][53] In cell culture studies, overexpression of hsa-mir-21-5p is associated with increased proliferation, migration, invasion, and colony formation as well as an decreased rate of apoptosis by targeting clusterin or PTEN. 17,18,22,23,51,54 Additionally, upregulated hsa-mir-21-5p has been shown to affect the well-known oncogenic WNT-β-catenin-pathway.…”

“…[15][16][17][18] In addition, the upregulation of hsa-mir-21-5p and of hsa-mir-155-5p have been described as diagnostic and prognostic markers in non-European HNSCC cohorts. [19][20][21][22][23][24][25] However, to the best of our knowledge, the prognostic impact of hsa-mir-21-5p and hsa-mir-155-5p in European OSCC cohorts is still unexplored.…”

13-gene DNA Methylation Analysis from Oral Brushing: A Promising Non Invasive Tool in the Follow-up of Oral Cancer Patients

“…Oral brushing sample collection was performed in the population study as previously described at two different times [10,15,21]:…”

miR‐146a promotes proliferation, invasion, and epithelial‐to‐mesenchymal transition in oral squamous carcinoma cells

“…In particular, the miRNA miR-146a is higher in the serum of patients with OSCC than in healthy controls. 13 miR-146a also inhibits growth of human hepatocellular carcinoma cell and is involved in anti-tumour immune suppression. However, it is still unclear whether miR-146a affects tumor growth or metastasis in OSCC.…”