A Noninvasive Method for Early Detection of MNNG-Induced Gastric Cancer of Male Wistar rat: Ultrasonic Study

Miri, H R; Bathaie, S. Zahra; Mohagheghi, Mohammad Ali; Mokhtari‐Dizaji, Manijhe; Shahbazfar, Amir Ali

doi:10.1016/j.ultrasmedbio.2010.11.015

Cited by 8 publications

(5 citation statements)

References 21 publications

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“…It has been shown that MNNG treatment results in the mutation of DNA and increased oxidative injury, which is critical to gastric carcinogenesis (10,11). Furthermore, the various stages before gastric cancer in Wistar rats are similar to the tissue morphology of humans (12). …”

Section: Introductionmentioning

confidence: 71%

miR-374 mediates the malignant transformation of gastric cancer-associated mesenchymal stem cells in an experimental rat model

Zhang

Qian

et al. 2017

Oncology Reports

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Mesenchymal stem cells (MSCs) are a critical component of the tumor microenvironment. Upon distinct pathological stimulus, MSCs show phenotypic and functional changes. Gastric cancer is one of the leading causes of cancer-related deaths worldwide. The roles and mechanisms of MSCs in gastric cancer have not been well characterized. In the present study, we investigated the roles of MSCs in the malignant transformation from gastritis to gastric cancer using an N-methyl-N'-nitro-N-nitrosoguanidine (MNNG)-induced gastric cancer model. We isolated MSCs from the gastric tissues of normal (RGN-MSCs) and MNNG-exposed rats (RGI-MSCs), and compared the biological properties of RGI-MSCs with RGN-MSCs. We found that RGI-MSCs had increased proliferative and migratory capabilities than these capacities noted in the RGN-MSCs. In addition, RGI-MSCs produced higher levels of IL-6, CXCL10 and MCP-1 than RGN-MSCs. Moreover, RGI-MSCs promoted the migration of normal gastric mucosa epithelial cells by inducing epithelial-mesenchymal transition (EMT). The upregulation of miR-374 in RGI-MSCs was partially responsible for their increased proliferative and migratory capabilities. Collectively, our findings provide new evidence for the roles of MSCs in gastric carcinogenesis, suggesting that targeting gastric cancer-associated MSCs may represent a novel avenue for gastric cancer therapy.

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Section: Introductionmentioning

confidence: 71%

miR-374 mediates the malignant transformation of gastric cancer-associated mesenchymal stem cells in an experimental rat model

Zhang

Qian

et al. 2017

Oncology Reports

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“…4,25,26 The rats in the control group were given distilled water. The experiment was terminated at the end of 48 th week and the stomachs of the rats injected with HPPH-CD (0.1, 0.5, and 1.0 mg kg À1 ) were imaged 48 h post drug injection.…”

Section: Mnng-induced Tumorigenesis and Uorescence Imagingmentioning

confidence: 99%

“…The rats in the model group were given MNNG at a concentration of 100 mg mL À1 in their drinking water via light-shielded bottles ad libitum for up to 47 weeks, according to the protocol described by previous studies. 4,25,26 The rats in the control group were given distilled water. The experiment was terminated at the end of 48 th week and the stomachs of the rats injected with HPPH-CD (0.1, 0.5, and 1.0 mg kg À1 ) were imaged 48 h post drug injection.…”

Section: Mnng-induced Tumorigenesis and Uorescence Imagingmentioning

confidence: 99%

“…3 However, being asymptomatic until an incurable advanced stage, more than one-third of gastric cancers are metastatic when diagnosed. 4,5 Surgery is currently considered an effective treatment modality for early-stage gastric cancer, yielding superior 5 year survival compared to other therapies. 6,7 An effective surgery, however, requires wide local resection of the primary tumor with clear surgical margins.…”

Section: Introductionmentioning

confidence: 99%

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Photodynamic diagnosis of gastric cancer using HPPH-CD

Huo

Yao²,

Liu

et al. 2016

RSC Adv.

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Gastric cancer is associated with a poor prognosis and is the fourth most common carcinoma and the second leading cause of cancer-related mortality worldwide due to its late-stage diagnosis and recurrence. New strategies are urgently needed to improve this condition. 2-[1 0 -Hexyloxyethyl]-2-devinyl pyropheophorbide-(HPPH)-cyanine dye (CD) mediated photodynamic diagnosis (PDD) has attracted great attention as a relatively new modality for tumor diagnosis. However, the efficacy of HPPH-CD-PDD on gastric cancer is poorly understood. The present study evaluates the effectiveness of PDD on gastric cancer diagnosis using HPPH-CD. We first evaluated the imaging potential of HPPH-CD on ICR mice bearing S180 subcutaneous tumors to make a preliminary definition of the ideal conditions for diagnosis. Subsequently, animal models with gastric cancer were established by giving 3,4-benzopyrene (B(a)P) to KM mice and 1-methyl-3-nitro-1-nitrosoguanidine (MNNG) to Wistar rats. The fluorescence signals of the stomach were then detected after HPPH-CD delivery, followed by imaging under a standard bright field. Furthermore, the tumors were collected, fixed and processed for histologic review. We found that subcutaneous S180 tumors and orthotopic primary gastric tumors could be easily visualized with fluorescence imaging after the delivery of HPPH-CD, and all malignant lesions with strong fluorescence were histologically confirmed. These results revealed the potential of HPPH-CD as a novel photosensitizer for the enhanced visualization of tumors in gastric cancer.

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“…A well-known carcinogen, N-methyl-N'-nitro-N-nitrosoguanidine (MNNG), has been broadly used to induce diverse cancer formation in normal cells and animals since its successful induction of stomach cancer formation in treated rats [2]. MNNG-induced cancer models have been used in determining the effects of Helicobacter pylori infection during cancer formation [3], screening of tumor suppressors [4], and early detection of cancer [5]. In addition, many scientific reports support that MNNG-induced cancer formation is a good mimetic model of animal cancer including humans [6,7].…”

Section: Introductionmentioning

confidence: 99%

MNNG-Regulated Differentially Expressed Genes that Contribute to Cancer Development in Stomach Cells

Kim

Jung

2021

Korean J Clin Lab Sci

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Cancer is a global health problem. There are diverse types of cancers, but there are several common pathways which lead to the development of cancer. Changes in gene expression might be the most common similarity found in almost all cancers. An understanding of the underlying changes in gene expression during cancer progression could lay a valuable foundation for the development of cancer therapeutics and even cancer vaccines. In this study, a well-known carcinogen, N-methyl-N'nitro-N-nitrosoguanidine (MNNG), was employed to induce changes in gene expression in normal stomach cells. MNNG is known to cause cancer by inducing damage to DNA in MNNG-treated mammalian cells and animals fed with this carcinogen. An analysis was performed by comparing the differentially expressed genes (DEGs) caused by MNNG treatment with DEGs in stomach cancer cell lines. To this end, methods of analysis for functional categorization and protein-protein interaction networks, such as gene ontology (GO), the database for annotation, visualization, and integrated discovery (DAVID), Kyoto encyclopedia of genes and genomics (KEGG) and search tool for the retrieval of interacting genes/proteins (STRING), were used. As a result of these analyses, MNNG-regulated specific genes and interaction networks of their protein products that contributed to stomach cancer were identified.

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A Noninvasive Method for Early Detection of MNNG-Induced Gastric Cancer of Male Wistar rat: Ultrasonic Study

Cited by 8 publications

References 21 publications

miR-374 mediates the malignant transformation of gastric cancer-associated mesenchymal stem cells in an experimental rat model

miR-374 mediates the malignant transformation of gastric cancer-associated mesenchymal stem cells in an experimental rat model

Photodynamic diagnosis of gastric cancer using HPPH-CD

MNNG-Regulated Differentially Expressed Genes that Contribute to Cancer Development in Stomach Cells

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