A noninvasive, direct real‐time PCR method for sex determination in multiple avian species

Brubaker, Jessica L.; Karouna‐Renier, Natalie K.; Chen, Yu; Jenko, Kathryn; Sprague, Daniel T.; Henry, Paula F. P.

doi:10.1111/j.1755-0998.2010.02951.x

Cited by 30 publications

(23 citation statements)

References 6 publications

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“…Birds from the captive colony were maintained on daily rations of dead laboratory mice (Mus musculus), dead chicken (Gallus gallus) hatchlings, or Classic Bird of Prey diet (Nebraska Brand, North Platte, NE) supplemented with Vionate Ò (Gimborn US, Atlanta, GA). Because screechowls are not sexually dimorphic, sex was determined by gene amplification (CHD1-Z and CHD1-W) using realtime PCR analysis (Brubaker et al 2011), and results were all confirmed at necropsy.…”

Section: Animalsmentioning

confidence: 99%

Assessment of toxicity and potential risk of the anticoagulant rodenticide diphacinone using Eastern screech-owls (Megascops asio)

Rattner

Horak²,

Lazarus

et al. 2012

Ecotoxicology

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In the United States, new regulatory restrictions have been placed on the use of some second-generation anticoagulant rodenticides. This action may be offset by expanded use of first-generation compounds (e.g., diphacinone; DPN). Single-day acute oral exposure of adult Eastern screech-owls (Megascops asio) to DPN evoked overt signs of intoxication, coagulopathy, histopathological lesions (e.g., hemorrhage, hepatocellular vacuolation), and/or lethality at doses as low as 130 mg/kg body weight, although there was no dose-response relation. However, this single-day exposure protocol does not mimic the multiple-day field exposures required to cause mortality in rodent pest species and non-target birds and mammals. In 7-day feeding trials, similar toxic effects were observed in owls fed diets containing 2.15, 9.55 or 22.6 ppm DPN, but at a small fraction (<5%) of the acute oral dose. In the dietary trial, the average lowest-observed-adverse-effect-level for prolonged clotting time was 1.68 mg DPN/kg owl/week (0.24 mg/kg owl/day; 0.049 mg/owl/day) and the lowest lethal dose was 5.75 mg DPN/kg owl/week (0.82 mg/kg owl/day). In this feeding trial, DPN concentration in liver ranged from 0.473 to 2.21 μg/g wet weight, and was directly related to the daily and cumulative dose consumed by each owl. A probabilistic risk assessment indicated that daily exposure to as little as 3-5 g of liver from DPN-poisoned rodents for 7 days could result in prolonged clotting time in the endangered Hawaiian short-eared owl (Asio flammeus sandwichensis) and Hawaiian hawk (Buteo solitarius), and daily exposure to greater quantities (9-13 g of liver) could result in low-level mortality. These findings can assist natural resource managers in weighing the costs and benefits of anticoagulant rodenticide use in pest control and eradication programs.

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Section: Animalsmentioning

confidence: 99%

Assessment of toxicity and potential risk of the anticoagulant rodenticide diphacinone using Eastern screech-owls (Megascops asio)

Rattner

Horak²,

Lazarus

et al. 2012

Ecotoxicology

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“…However, considerable variation may occur in the quantity and the quality of the recovered DNA, depending on whether the samples are fresh plucked feathers (Harvey et al 2006) or shed feathers (Hogan et al 2008). Buccal cells collected using cotton swabs provide another source of DNA that is now regularly used in a number of species: amphibians (Pidancier et al 2003;Broquet et al 2007a), fish (Smalley & Campanella 2005), mammals (Mitrecic et al 2008) and more rarely birds (Bush et al 2005;Handel et al 2006;Brubaker et al 2011). Nondestructive and noninvasive samples such as feathers and buccal swabs may contain small quantities of DNA and ⁄ or degraded DNA.…”

Section: Introductionmentioning

confidence: 99%

Description of microsatellite markers and genotyping performances using feathers and buccal swabs for the Ivory gull (Pagophila eburnea)

Yannic¹,

Sermier²,

Aebischer³

et al. 2011

Molecular Ecology Resources

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We report 22 new polymorphic microsatellites for the Ivory gull (Pagophila eburnea), and we describe how they can be efficiently co-amplified using multiplexed polymerase chain reactions. In addition, we report DNA concentration, amplification success, rates of genotyping errors and the number of genotyping repetitions required to obtain reliable data with three types of noninvasive or nondestructive samples: shed feathers collected in colonies, feathers plucked from living individuals and buccal swabs. In two populations from Greenland (n=21) and Russia (Severnaya Zemlya Archipelago, n=21), the number of alleles per locus varied between 2 and 17, and expected heterozygosity per population ranged from 0.18 to 0.92. Twenty of the markers conformed to Hardy-Weinberg and linkage equilibrium expectations. Most markers were easily amplified and highly reliable when analysed from buccal swabs and plucked feathers, showing that buccal swabbing is a very efficient approach allowing good quality DNA retrieval. Although DNA amplification success using single shed feathers was generally high, the genotypes obtained from this type of samples were prone to error and thus need to be amplified several times. The set of microsatellite markers described here together with multiplex amplification conditions and genotyping error rates will be useful for population genetic studies of the Ivory gull.

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“…They were then immersed in a 30% sucrose solution for approximately 30 h to ensure cryoprotection (until the brains sank to the bottom of the vial) and then frozen at -80 8C. The sex of the hatchlings was determined genetically following the method of Brubaker et al (2011). The sex of the hatchlings was determined genetically following the method of Brubaker et al (2011).…”

Section: Egg Injections Experimental Groups and Tissue Collectionmentioning

confidence: 99%

“…The brains were shipped frozen on dry ice from USGS Patuxent Wildlife Research Center to McGill University, where they were stored at -80 8C until they were processed for neuroanatomical measurements as described below (Histological processing of brains) following Guigueno et al (2018Guigueno et al ( , 2016. The sex of the hatchlings was determined genetically following the method of Brubaker et al (2011).…”

Section: Egg Injections Experimental Groups and Tissue Collectionmentioning

confidence: 99%

Sex‐specific responses in neuroanatomy of hatchling American kestrels in response to embryonic exposure to the flame retardants bis(2‐ethylhexyl)‐2,3,4,5‐tetrabromophthalate and 2‐ethylhexyl‐2,3,4,5‐tetrabromobenzoate

Guigueno

Karouna‐Renier

Henry

et al. 2018

Enviro Toxic and Chemistry

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Bis(2-ethylhexyl)-2,3,4,5-tetrabromophthalate (BEH-TEBP) and 2-ethylhexyl-2,3,4,5-tetrabromobenzoate (EH-TBB), flame retardant components of FireMaster 550® and 600® have been detected in tissues of wild birds. To address the paucity of information regarding potential impacts of flame retardants on the brain, brain volume regions of hatchling American kestrels (Falco sparverius) were evaluated following in ovo injection at embryonic day 5 with safflower oil or to 1 of 3 doses of either BEH-TEBP (13, 64, or 116 μg/g egg) or EH-TBB (12, 60, or 149 μg/g egg). The doses for both chemicals reflected concentrations reported in wild birds. The volumes of the hippocampus and telencephalon and volumetric differences between left and right hemispheres were measured in hatchlings (embryonic day 28). A sex-specific effect of BEH-TEBP on relative hippocampus volume was evident: the hippocampus was significantly enlarged in high-dose females compared to control females but smaller in low-dose females than the other females. There was no significant effect of EH-TBB on hippocampus volume in female kestrel hatchlings or of either chemical in male hatchlings and no effects of these concentrations of EH-TBB or BEH-TEBP on telencephalon volume or the level of symmetry between the hemispheres of the brain. In sum, embryonic exposure of female kestrels to these BEH-TEBP concentrations altered hippocampus volume, having the potential to affect spatial memory relating to ecologically relevant behavior such as prey capture, predator avoidance, and migration. Environ Toxicol Chem 2018;37:3032-3040. © 2018 SETAC.

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A noninvasive, direct real‐time PCR method for sex determination in multiple avian species

Cited by 30 publications

References 6 publications

Assessment of toxicity and potential risk of the anticoagulant rodenticide diphacinone using Eastern screech-owls (Megascops asio)

Assessment of toxicity and potential risk of the anticoagulant rodenticide diphacinone using Eastern screech-owls (Megascops asio)

Description of microsatellite markers and genotyping performances using feathers and buccal swabs for the Ivory gull (Pagophila eburnea)

Sex‐specific responses in neuroanatomy of hatchling American kestrels in response to embryonic exposure to the flame retardants bis(2‐ethylhexyl)‐2,3,4,5‐tetrabromophthalate and 2‐ethylhexyl‐2,3,4,5‐tetrabromobenzoate

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