A Noninfusion Blood Agar Base for Neisseriae, Pneumococci and Streptococci

Haemophilus Vaginalis

“…Thc first recognizable description of H. vaginalis was by Leopold (1953), working in the U.S.A., who reported the isolation of small Gram negative aerobic bacilli froin the urine of 53 of 965 males with mild prostatitis, and from 16 of 58 cervical swabs from cases of cervicitis. Growth occurred as pinpoint haeniolytic colonies after 48 hours incubation on Casman's blood agar (Casman, 1947). Leopold suggested a close relationship of this organism with the genus "Haemophilus", but did not name its species.…”

The Inhibition of Haemophilus Influenzae By Certain Agar and Peptone Preparations

“…was first observed by Avery in 1918; Butler (1962) found that this substance could replace serum albumen in a semidefined medium for Haemophilus and suggested that its role may be that of a protective colloid. Albumen, starch and charcoal have a similar effect in media for the growth of other pathogens (Dubos and Davis, 1946;Casman, 1947;Mazloum and Rowley, 1955). Our previous findings with regard to the growth of certain strains of H. paruinfluenzue (Evans and Smith, 1972) indicate that sodium oleate may be a useful additive for media used to grow members of the genus Haemophilus.…”

“…The inoculum required to initiate growth increased from less than 10 cells to 107, the colonies were of irregular size and microscopically many filamentous forms were evident. The constituents of both satisfactory and unsatisfactory media appeared to be identical, and preliminary investigations suggested that the duration and temperature of autoclaving might adversely affect the medium.Inhibitory effects of media have been attributed to a number of agents including copper, colloidal sulphur, peroxides, fatty acids, certain amino acids and other substances (Burnet, 1925;Gordon and McLeod, 1926;O'Meara and Macsween, 1936;Ley and Mueller, 1946;Casman, 1947;Proom et al, 1950;Nieman, 1954;Woiwod, 1954;Traxler and Lankford, 1957;Dukes and Gardner, 1961). This paper describes an extension of earlier studies on proteose peptone as a basal medium for H. infuenzae and the value of dithionite and sodium oleate as additives to neutralise potential inhibitors.…”

Synthesis and biological studies of some new acrylic acid ethyl esters of quinolinone

“…The antibacterial (against Escherichia coli and Bacillus cirrhosis) and antifungal (against Aspergillus niger and Rhizoctonia bataticola) activities of the compounds were tested at three concentrations (25, 50, and 100 lg cm -3 ) using norfloxacin and griseofulvin as standard antibacterial and antifungal drugs, respectively [16][17][18]. Tables 1 and 2 list the zones of growth inhibition (in millimeters and as percentage values) obtained against the tested bacteria and fungi.…”

“…DMF was used as a solvent control, and the reference drugs used were norfloxacin and griseofulvin. The tests were carried out by the cup-plate method [16][17][18], at a concentration of 100, 50, and 25 lg cm -3 . The zone of inhibition was measured in millimeters after 48 h of incubation at 37°C.…”