A Nondenaturing Purification Scheme for the DNA-Binding Domain of Poly(ADP-Ribose) Polymerase, a Structure-Specific DNA-Binding Protein

Ruscetti, Tracy; Newman, Janet; Peat, Thomas S.; francis, J.A.; Nolan, Rhiannon; Terwilliger, Thomas C.; Peterson, Scott; Lehnert, Bruce E.

doi:10.1006/prep.1998.0919

Cited by 6 publications

(1 citation statement)

References 26 publications

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“…Specificity of monoclonal antibodies directed against PARP‐1, p89, and pro‐caspase 3 was confirmed on western blot analysis. Bands of the appropriate size were detected, conforming to the literature (Figure 2)17, 29. Immunoprecipitation using the anti‐active caspase 3 antibody C92‐605, followed by detection with antibody CPP32 recognising both pro‐ and active caspase 3, revealed a band at 17 kD (active caspase 3) only in the induced cells and not in the negative controls (Figure 2B).…”

Section: Resultssupporting

confidence: 87%

Mouse mutant “rib‐vertebrae” (rv): A defect in somite polarity

Nacke

Schafer

Angelis³

et al. 2000

Dev. Dyn.

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Section: Resultssupporting

confidence: 87%

Mouse mutant “rib‐vertebrae” (rv): A defect in somite polarity

Nacke

Schafer

Angelis³

et al. 2000

Dev. Dyn.

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Apoptosis in B‐cell lymphomas and reactive lymphoid tissues always involves activation of caspase 3 as determined by a new in situ detection method

Dukers

Oudejans

Vos

et al. 2002

The Journal of Pathology

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In vitro studies indicate that in lymphomas, execution of apoptosis involves activation of effector caspases. To investigate activation of effector caspases in vivo in biopsy specimens of lymphomas, a new assay was developed using antibodies against active caspase 3 and p89, a protein fragment generated by caspase-specific cleavage of poly-ADP ribose polymerase (PARP). Using this assay, it was found that in B-cell lymphomas, levels of active caspase 3/p89-positive cells correlate strongly with morphologically recognizable apoptotic cells. The number of active caspase 3/p89-positive cells was low in follicular lymphomas and usually high in diffuse large cell lymphomas. Highest numbers were found in Burkitt lymphomas and in two biopsies of diffuse large B-cell lymphomas (DLCLs) obtained several days after initiation of therapy. It is concluded that apoptosis in reactive lymphoid tissues and in B-cell lymphomas always involves activation of effector caspase 3 and cleavage of one of the major effector caspase substrates, PARP-1. Moreover, levels of effector caspase activation are constantly low in low-grade follicular lymphomas and vary considerably in DLCL and Burkitt lymphoma.

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Production, Extraction, and Purification of Human Poly(ADP-ribose) Polymerase-1 (PARP-1) with High Specific Activity

Knight¹,

Chambers

2001

Protein Expression and Purification

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A Nondenaturing Purification Scheme for the DNA-Binding Domain of Poly(ADP-Ribose) Polymerase, a Structure-Specific DNA-Binding Protein

Cited by 6 publications

References 26 publications

Mouse mutant “rib‐vertebrae” (rv): A defect in somite polarity

Mouse mutant “rib‐vertebrae” (rv): A defect in somite polarity

Apoptosis in B‐cell lymphomas and reactive lymphoid tissues always involves activation of caspase 3 as determined by a new in situ detection method

Production, Extraction, and Purification of Human Poly(ADP-ribose) Polymerase-1 (PARP-1) with High Specific Activity

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