A non-steroidal anti-inflammatory drug (ketoprofen) does not delay β-TCP bone graft healing

Nyangoga, Hervé; Aguado, Eric; Goyenvalle, Eric; Basle, Michel‐Félix; Chappard, Daniel

doi:10.1016/j.actbio.2010.01.042

Cited by 39 publications

(34 citation statements)

References 45 publications

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“…Numerous studies have reported that the biocompatible and osteoconductive b-TCP scaffolds could support the attachment, proliferation, and differentiation of osteogenic cells (such as osteoblasts and bMSCs). 27,[33][34][35][36] In agreement with these literatures, the microscopy analysis of the bMSCs/b-TCP constructs in this study also showed that the cells could adhere to the surface of the scaffold, spread, and proliferate. As in regular culture on tissue culture plastic, the bMSCs cultured on b-TCP scaffold displayed the expected osteogenic activity when cultured in the osteogenic medium.…”

Section: Discussionsupporting

confidence: 91%

Orthodontic Tooth Movement in Alveolar Cleft Repaired with a Tissue Engineering Bone: An Experimental Study in Dogs

Zhang

Chu

Yang

et al. 2011

Tissue Engineering Part A

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Tissue engineering approaches have been successfully used in repairing bone defects and have become a viable alternative to autologous bone. The aim of the present study was to investigate if a construct of porous beta-tricalcium phosphate (β-TCP) combined with osteogenically induced bone marrow stromal cells (bMSCs) could repair alveolar cleft, and allow for subsequent orthodontic tooth movement in a canine model. Twelve alveolar osteotomy surgeries in six animals were made bilaterally and randomly implanted by (1) tissue-engineered bone complex of bMSCs/β-TCP (group A, n=4), (2) β-TCP alone (group B, n=4), and (3) autologous bone obtained from iliac bone (group C, n=4). Contralateral alveolar defects were created in one animal and left untreated to serve as blank control to observe spontaneous healing of the defects. Sequential fluorescent labeling and radiographic observation was used to evaluate new bone formation and mineralization in each defect. Orthodontic tooth movement was initiated 8 weeks after surgical operation for 12 weeks, and then the dogs were sacrificed for histological and histomorphometric analysis. Results indicated that the tissue-engineered complex with bMSCs/β-TCP dramatically promoted new bone formation and mineralization and achieved a favorable height of the repaired alveolar when compared with β-TCP alone, which absorbed severely. The overall effect of the tissue-engineered bone was equivalent to autologous bone; the physiological function of the alveolar bone was restored by allowing the adjacent teeth to move into the newly formed bone in the grafted region. This study demonstrated that the tissue engineering bone from the combination of β-TCP and bMSCs is a feasible clinical approach for patients with alveolar cleft and the subsequent orthodontic tooth movement.

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Section: Discussionsupporting

confidence: 91%

Orthodontic Tooth Movement in Alveolar Cleft Repaired with a Tissue Engineering Bone: An Experimental Study in Dogs

Zhang

Chu

Yang

et al. 2011

Tissue Engineering Part A

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“…An in vivo study evidenced active osteoblasts on newly formed trabeculae and active osteoclasts 2 weeks after implantation β‐TCP particles in the rat 13. Similarly, our group evidenced the centripetal development of new bone trabeculae by microcomputed tomography as early as 8 days after implantation in the femoral condyle of the rabbit 26. After a 3‐month implantation period, the extensive formation of bone trabeculae formed onto and around β‐TCP particles is reported in a number of studies 27–30.…”

Section: Discussionsupporting

confidence: 75%

In vitro assessment of osteoblast and macrophage mobility in presence of β‐TCP particles by videomicroscopy

Beuvelot

Pascaretti-Grizon

Filmon

et al. 2010

J Biomedical Materials Res

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β-TCP is widely used to repair bone defects due to its good biocompatibility, macroporosity (favoring bone ingrowth) and bioresorbability. However, cell interactions with the biomaterial at the first times of implantation remain largely unknown. We have observed cell behaviors in direct contact with β-TCP particles using long-term culture under videomicroscopy. Osteoblastlike cells (SaOs-2) and macrophages (J774.2 and mouse peritoneal macrophages) were cultured in the presence of β-TCP particles. For each experiment, images from 20 independent fields were acquired and stored every 15 min during 8 days. At the end of the culture, they were combined to generate time lapse videos; coverslips were fixed and observed by scanning electron microscopy (SEM). SaOs-2 proliferation was determined by counting cells on six different and independent fields at days 1, 3, and 6. Videos showed the capacity of cells to displace the particles. Dynamic follow-up showed active proliferation of SaOs-2 occurring in the direction of the particles. J774.2 and peritoneal macrophages did not proliferate but came in direct contact with the particles and actively eroded them. SEM showed that cells were stretched and fixed onto the surface and seemed to climb from the coverslip to the particles. The long-term culture under videomicroscopy allowed a better understanding of the colonization process of β-TCP particles by osteoblastlike cells and macrophages. Data obtained from long-term videomicroscopy are in agreement with in vivo observations confirming the interest of β-TCP to promote osteogenesis.

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“…Analysing these studies further, we could not identify any association among the class or potency of the studied NSAIDs to inhibit the COX-1 or COX-2 enzyme, the dose, or the timing. In terms of timing, although some authors suggest that short-term administration after fracture could be safe, others contradict this finding suggesting that NSAID administration is safe only if it is initiated a few weeks after fracture [78, 85, 98, 101, 105, 106, 108, 126]. A link, however, can exist between the size of the animals.…”

Section: Resultsmentioning

confidence: 99%

Do Nonsteroidal Anti-Inflammatory Drugs Affect Bone Healing? A Critical Analysis

Pountos

Georgouli

Calori

et al. 2012

The Scientific World Journal

232

162

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Nonsteroidal anti-inflammatory drugs (NSAIDs) play an essential part in our approach to control pain in the posttraumatic setting. Over the last decades, several studies suggested that NSAIDs interfere with bone healing while others contradict these findings. Although their analgesic potency is well proven, clinicians remain puzzled over the potential safety issues. We have systematically reviewed the available literature, analyzing and presenting the available in vitro animal and clinical studies on this field. Our comprehensive review reveals the great diversity of the presented data in all groups of studies. Animal and in vitro studies present so conflicting data that even studies with identical parameters have opposing results. Basic science research defining the exact mechanism with which NSAIDs could interfere with bone cells and also the conduction of well-randomized prospective clinical trials are warranted. In the absence of robust clinical or scientific evidence, clinicians should treat NSAIDs as a risk factor for bone healing impairment, and their administration should be avoided in high-risk patients.

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A non-steroidal anti-inflammatory drug (ketoprofen) does not delay β-TCP bone graft healing

Cited by 39 publications

References 45 publications

Orthodontic Tooth Movement in Alveolar Cleft Repaired with a Tissue Engineering Bone: An Experimental Study in Dogs

Orthodontic Tooth Movement in Alveolar Cleft Repaired with a Tissue Engineering Bone: An Experimental Study in Dogs

In vitro assessment of osteoblast and macrophage mobility in presence of β‐TCP particles by videomicroscopy

Do Nonsteroidal Anti-Inflammatory Drugs Affect Bone Healing? A Critical Analysis

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