A non-invasive test for sex identification in Short-toed Eagle (Circaetus gallicus)

Sacchi, Paola; Soglia, Dominga; Maione, Sandra; Meneguz, G.; Campora, Massimo; Rasero, Roberto

doi:10.1016/j.mcp.2004.01.002

Cited by 49 publications

(45 citation statements)

References 11 publications

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“…The CHD gene has been used successfully in many bird species , Miyaki et al 1998, Ito et al 2003, Sacchi et al 2004, Lee et al 2007, 2010, since the gene is preserved in most bird species (Griffiths & Tiwari 1995).…”

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confidence: 99%

Sex determination in Turdus amaurochalinus (Passeriformes: Muscicapidae): morphometrical analysis supported by CHD gene

Silva

Lôbo-Hajdu²,

Alves³

2010

RBT

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Sex determination is important for conservation and population studies, particularly for reproduction programs of threatened species and behavioural ecology. Turdus amaurochalinus, Creamy-bellied Thrush, only exhibits sexual dimorphism during the breeding season, when males are considered to show intense yellow bills, and females and immature males show dark brown bills. The objectives of this study were: 1) to determine the sex of individuals using genetic techniques, and 2) to test the hypothesis that sex dimorphism can be detected by morphometry. This study was carried out at Parque Nacional da Restinga de Jurubatiba, a preserved area located on the North coast of Rio de Janeiro State. The birds were captured using ornithological nets, singly marked with metal rings, weighed, measured and had blood samples collected before being released. The sex of 42 T. amaurochalinus individuals was determined using the CHD gene marker. A total of 20 males and 22 females were identified from June to August, with peak capture frequency in June. Turdus amaurochalinus females and males differed significantly in morphometrical measures. The most important traits to distinguish males from females were wing length (Student t-test=4.34, df=40, p=0.0001) and weight (Student t-test=2.08, df=40, p=0.044): females were heavier and had significantly shorter wing length than males. Females and males were correctly classified in 86% and 75% of cases, respectively, using Discriminant Analysis. The molecular analysis was the most secure method for sex determination in the studied species. Rev. Biol. Trop. 59 (2): 789-794. Epub 2011 June 01.

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confidence: 99%

Sex determination in Turdus amaurochalinus (Passeriformes: Muscicapidae): morphometrical analysis supported by CHD gene

Silva

Lôbo-Hajdu²,

Alves³

2010

RBT

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“…In this study, the use of feathers as a source of DNA has been proven as an efficient and effective technique for genetic sexing of the white-bellied sea eagle. This technique has also been applied in other Falconiformes species (see e.g., Sacchi et al, 2004; Rudnick et al, 2007;Ong and Vellayan, 2008). Because avian blood cells are nucleated, the total amount of DNA extracted from feathers seems to be sufficient for conducting this research (Ong and Vellayan, 2008).…”

Section: Discussionmentioning

confidence: 99%

“…Total genomic DNA was extracted using the Tissue DNA kit (Genispin) according to manufacturer instructions with modifications of the pre-lysis step as described by Sacchi et al (2004).…”

Section: Dna Extraction and Pcr Amplificationsmentioning

confidence: 99%

“…A crucial point in any kind of free-ranging animals is the sampling that must be simple, not stressing and non-invasive (Sacchi et al, 2004). Surgical procedures such as laparoscopy are intrusive and represent a risk to the individual; consequently, such techniques are generally avoided with endangered species (Millar et al, 1997;Griffiths et al, 1998).…”

Section: Introductionmentioning

confidence: 99%

“…The most widely used molecular methods for sexing birds exploit the size differences between introns of the CHD gene on the Z-and W-sex chromosomes (Shizuka and Lyon, 2008 and references therein). This PCR test employs a single set of primers that amplify homologous fragments of both the CHD-W gene, unique to female, and CHD-Z gene, occurring in both sexes (Sacchi et al, 2004). Thus, the aim of our study was, through the utilization of a non-invasive sampling technique, to describe sex identification of H. leucogaster using this PCR-based test.…”

Section: Introductionmentioning

confidence: 99%

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Short Communication Non-invasive sex identification of the white-bellied sea eagle (Haliaeetus leucogaster) through genetic analysis of feathers

Naim¹,

Nor²,

Baharuddin³

2011

Genet. Mol. Res.

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ABSTRACT. The white-bellied sea eagle, Haliaeetus leucogaster, displays reversed sexual size dimorphism and is monomorphic for adult plumage coloration. Early attempts to identify sex in sexually monomorphic birds were based on morphological or chromosomal characters, but since avian W-specific DNA sequences were identified, PCR amplification has become commonly used for molecular sexing. We used a PCR test employing primers that amplify two homologous fragments of both the CHD-W gene, unique to females, and the CHD-Z gene, occurring in both sexes. This test was applied to five individuals of H. leucogaster from the Malacca Zoo and to male and female domestic chickens, Gallus domesticus, for comparison. All individuals were sexed successfully with high reproducibility. We conclude that this PCR-based test with feathers as the DNA source is a reliable sexing method for H. leucogaster. This sexing technique is objective and non-invasive and could be used to test sex ratio theories, as well as to help improve conservation and management actions for captive breeding program of this species in Malaysia.

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A new marker based on the avian spindlin gene that is able to sex most birds, including species problematic to sex with CHD markers

2016

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We have developed a new marker (Z43B) that can be successfully used to identify the sex of most birds (69%), including species difficult or impossible to sex with other markers. We utilized the zebra finch Taeniopygia guttata EST microsatellite sequence (CK309496) which displays sequence homology to the 5 0 untranslated region (UTR) of the avian spindlin gene. This gene is known to be present on the Z and W chromosomes. To maximize cross-species utility, the primer set was designed from a consensus sequence created from homologs of CK309496 that were isolated from multiple distantly related species. Both the forward and reverse primer sequences were 100% identical to 14 avian species, including the Z chromosome of eight species and the chicken Gallus gallus W chromosome, as well as the saltwater crocodile Crocodylus porosus. The Z43B primer set was assessed by genotyping individuals of known sex belonging to 61 non-ratite species and a single ratite. The Z and W amplicons differed in size making it possible to distinguish between males (ZZ) and females (ZW) for the majority (69%) of non-ratite species tested, comprising 10 orders of birds. We predict that this marker will be useful for obtaining sex-typing data for ca 6,869 species of birds (69% of non-ratites but not galliforms). A wide range of species could be sex-typed including passerines, shorebirds, eagles, falcons, bee-eaters, cranes, shags, parrots, penguins, ducks, and a ratite species, the brown kiwi, Apteryx australis. Those species sexed include species impossible or problematic to sex-type with other markers (magpie, albatross, petrel, eagle, falcon, crane, and penguin species). Zoo Biol. 35:533-545, 2016.

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A non-invasive test for sex identification in Short-toed Eagle (Circaetus gallicus)

Cited by 49 publications

References 11 publications

Sex determination in Turdus amaurochalinus (Passeriformes: Muscicapidae): morphometrical analysis supported by CHD gene

Sex determination in Turdus amaurochalinus (Passeriformes: Muscicapidae): morphometrical analysis supported by CHD gene

Short Communication Non-invasive sex identification of the white-bellied sea eagle (Haliaeetus leucogaster) through genetic analysis of feathers

A new marker based on the avian spindlin gene that is able to sex most birds, including species problematic to sex with CHD markers

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