A non-invasive method for the assessment of hemostasis in vivo by using dynamic light scattering

Abstract

We present a new non invasive method for assessing hemostasis in vivo. This method is based on the analysis of the movement characteristics of red blood cells (RBCs) during blood stasis condition. Stasis is intermittently induced by occlusion of arterial blood flow at the finger root. We assumed that under zero flow conditions, RBC movement is driven mostly by Brownian motion, and we characterized the RBC movement by utilizing the dynamic light scattering (DLS) technique in vivo. We found that during the stasis the RBCs diffusion coefficient in plasma decreases. We speculate that the RBC diffusion coefficient is most strongly related to endothelial and hemostatic activity. This assumption is supported by our findings that RBC move ment, being expressed through the characteristics of the measured DLS signal, is correlative to the biological age and also is related to the coagulation factors. This new method can serve as a new diagnostic and research tool for the assessment of hemostasis and vascular function.

Impact of Fitness Status on the Optically Measured Hemodynamic Indexes

“…The miniaturized dynamic light-scattering sensor (mDLS) is designed to measure different parameters of the cutaneous blood flow [7]. Laser light is scattered from tissue and moving red blood cells (RBC).…”

“…By using the power spectrum ( P ) presentation of (4), the hemodynamic index (HI) can be defined by [7, 10]: $\begin{array}{c}true\mathrm{H}\mathrm{I}\left(\left[f_1,f_2\right],t\right)={\int }_{f_1}^{f_2}P\left(\omega ,t\right)d\omega ,\end{array}$where f 1 and f 2 are defined as chosen bandpass bounders.…”

New noninvasive index for evaluation of the vascular age of healthy and sick people

“…The in vivo DLS measurement system (ELFI-TECH3) 7 consists of three parts: the probe unit, the acquisition and processing system, and the electronic and control unit. The probe unit includes the 850-nm vertical cavity surface emitting laser, the pressurizing assembly, detector, and amplifier.…”

“…4,5 In our previous studies [6][7][8] we measured intravascular mobility of RBC as a natural marker of the effective viscosity of the blood plasma in close proximity to the vascular wall. For this end, we utilized DLS 9 for measurement of the RBC diffusion coefficient under the condition of intermittent stasis.…”

Investigating tissue respiration and skin microhaemocirculation under adaptive changes and the synchronization of blood flow and oxygen saturation rhythms

“…This has led to the development and introduction of novel and compact non-invasive optical diagnostic devices into biomedicine. Especially, progress has been achieved in developing methods and devices for non-invasive studies of blood flow (Zakharov et al 2009(Zakharov et al , 2011 as well as quantitative assessment and imaging associated with this (Kalchenko et al 2011, 2012, Fine et al 2012, Daly et al 2013. However, these methods and devices do not allow full analysis of the consumption and utilization of oxygen in tissue, as well as frequency modulation rhythms of blood flow and tissue oxygen saturation.…”