A non‐fatal case of invasive zygomycete (<i><scp>L</scp>ichtheimia corymbifera</i>) infection in an allogeneic haematopoietic cell transplant recipient

Eickhardt, Steffen; Braendstrup, Peter; Clasen‐Linde, Erik; Jensen, Karl Erik; Alhede, Morten; Bjarnsholt, Thomas; Høiby, Niels; Vindeløv, Lars L.; Moser, Claus

doi:10.1111/apm.12008

Cited by 3 publications

(1 citation statement)

References 11 publications

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“…In the present study, the CotH family of proteins was found to be highly conserved among Mucorales organisms that are known to cause invasive disease (Table 1). CotH proteins were present in Mucor spp., Lichtheimia, Rhizomucor, Mortierella, and Lentamyces, which are known to cause invasive infections to mammals and to other Mucorales (29)(30)(31)(32)(33)(34)(35). Notably, the CotH family was not present in any other organisms, including Conidiobolus coronatus, which was previously classified as a Zygomycete and causes superficial infections (2).…”

Section: Figurementioning

confidence: 99%

CotH3 mediates fungal invasion of host cells during mucormycosis

Gebremariam¹,

Liu²,

Luo³

et al. 2013

J. Clin. Invest.

199

294

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Angioinvasion is a hallmark of mucormycosis. Previously, we identified endothelial cell glucose-regulated protein 78 (GRP78) as a receptor for Mucorales that mediates host cell invasion. Here we determined that spore coat protein homologs (CotH) of Mucorales act as fungal ligands for GRP78. CotH proteins were widely present in Mucorales and absent from noninvasive pathogens. Heterologous expression of CotH3 and CotH2 in Saccharomyces cerevisiae conferred the ability to invade host cells via binding to GRP78. Homology modeling and computational docking studies indicated structurally compatible interactions between GRP78 and both CotH3 and CotH2. A mutant of Rhizopus oryzae, the most common cause of mucormycosis, with reduced CotH expression was impaired for invading and damaging endothelial cells and CHO cells overexpressing GRP78. This strain also exhibited reduced virulence in a diabetic ketoacidotic (DKA) mouse model of mucormycosis. Treatment with anti-CotH Abs abolished the ability of R. oryzae to invade host cells and protected DKA mice from mucormycosis. The presence of CotH in Mucorales explained the specific susceptibility of DKA patients, who have increased GRP78 levels, to mucormycosis. Together, these data indicate that CotH3 and CotH2 function as invasins that interact with host cell GRP78 to mediate pathogenic host-cell interactions and identify CotH as a promising therapeutic target for mucormycosis.

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Section: Figurementioning

confidence: 99%

CotH3 mediates fungal invasion of host cells during mucormycosis

Gebremariam¹,

Liu²,

Luo³

et al. 2013

J. Clin. Invest.

199

294

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A 3D‐Printed Customizable Platform for Multiplex Dynamic Biofilm Studies

Dhall

Ramjee

et al. 2022

Adv Materials Technologies

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Biofilms are communities of microbes that colonize surfaces. While several biofilm experimental models exist, they often have limited replications of spatiotemporal dynamics surrounding biofilms. For a better understanding dynamic and complex biofilm development, this manuscript presents a customizable platform compatible with off‐the‐shelf well plates that can monitor microbial adhesion, growth, and associated parameters under various relevant scenarios by taking advantage of 3D printing. The system i) holds any substrate in a stable, vertical position, ii) subjects samples to flow at different angles, iii) switches between static and dynamic modes during an experiment, and iv) allows multiplexing and real‐time monitoring of biofilm parameters. Simulated fluid dynamics is employed to estimate flow patterns around discs and shear stresses at disc surfaces. A 3D printed peristaltic pump and a customized pH measurement system for real‐time tracking of spent biofilm culture media are equipped with a graphical user interface that grants control over all experimental parameters. The system is tested under static and dynamic conditions with Streptococcus mutans using different carbon sources. By monitoring the effluent pH and characterizing biochemical, microbiological, and morphological properties of cultured biofilms, distinct properties are demonstrated. This novel platform liberates designing experimental strategies for investigations of biofilms under various conditions.

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First Case of Rhinocerebral Mucormycosis Caused by Lichtheimia ornata, with a Review of Lichtheimia Infections

Pan

Tsui

et al. 2020

Mycopathologia

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A non‐fatal case of invasive zygomycete (Lichtheimia corymbifera) infection in an allogeneic haematopoietic cell transplant recipient

Cited by 3 publications

References 11 publications

CotH3 mediates fungal invasion of host cells during mucormycosis

CotH3 mediates fungal invasion of host cells during mucormycosis

A 3D‐Printed Customizable Platform for Multiplex Dynamic Biofilm Studies

First Case of Rhinocerebral Mucormycosis Caused by Lichtheimia ornata, with a Review of Lichtheimia Infections

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