A Non-Degradative Extraction Method for Molecular Structure Characterization of Bacterial Glycogen Particles

Methods for Studying Glycogen Metabolism

“…Traditional methods for isolating glycogen are sufficient for measuring the glycogen concentration, but they compromise the fine structure of the glycogen, meaning the development of a milder technique is required. A cold-water extraction using sucrose density gradient centrifugation is described for use in bacteria by Wang, Wang et al 6 . This technique is superior to other methods in preserving both larger glycogen molecules, as measured by size exclusion chromatography (SEC), and longer chains, as measured by fluorophore-assisted capillary electrophoresis (FACE) 6 .…”

“…A cold-water extraction using sucrose density gradient centrifugation is described for use in bacteria by Wang, Wang et al 6 . This technique is superior to other methods in preserving both larger glycogen molecules, as measured by size exclusion chromatography (SEC), and longer chains, as measured by fluorophore-assisted capillary electrophoresis (FACE) 6 .…”