A non-curved chicken lysozyme 5' matrix attachment site is 3' followed by a strongly curved DNA sequence

Kries, Jens Peter von; Phi‐van, Loc; Diekmann, Stephen; Strätling, Wolf H.

doi:10.1093/nar/18.13.3881

Cited by 67 publications

(42 citation statements)

References 33 publications

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“…This construct was made by use of standard molecular biology techniques 22 and was grown in Escherichia coli strain STBL2 (Life Technologies). We coinjected a 2-fold molar excess of the Ϸ3-kb chicken lysozyme matrix attachment region 23 to improve specificity of transgene expression. 24 Transgenic mice were prepared in the University Transgenic Mouse Facility, with FVB/N mice used as donors and recipients.…”

Section: Transgenic Mice Expressing Apoe In the Adrenal Glandmentioning

confidence: 99%

Low Levels of Extrahepatic Nonmacrophage ApoE Inhibit Atherosclerosis Without Correcting Hypercholesterolemia in ApoE-Deficient Mice

Thorngate

Rudel

Walzem

et al. 2000

ATVB

108

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Abstract-The prevention of atherosclerosis by apolipoprotein E (apoE) is generally attributed to the removal of plasma lipoprotein remnant particles. We developed transgenic apoE-knockout mice expressing apoE specifically in the adrenal gland and found that only 3% of the wild-type plasma level of apoE was sufficient to normalize plasma cholesterol levels in the apoE-deficient mouse. As expected, mice expressing apoE at levels that correct hypercholesterolemia had almost no cholesteryl ester deposition in their aortas. In contrast, their nontransgenic siblings had significant atherosclerosis. Unexpectedly, we found that atherosclerosis was also reduced in 2 transgenic lines expressing too little apoE (Ͻ1% to 2% of wild type) to correct their hypercholesterolemia. Gel exclusion chromatographic profiles of plasma lipoproteins and the size distributions of lipoproteins with density Ͻ1.063 (low density and very low density lipoproteins), as determined by dynamic laser light scattering, were the same in mice expressing Ͻ2 g/mL plasma apoE and their nontransgenic littermates. We conclude that the antiatherogenic action of low levels of plasma apoE is not due to the clearance of remnant lipoproteins. Thus, low levels of apoE provided systemically, but not made in the liver or in macrophages, can block atherogenesis in the vascular wall independently of normalizing the plasma concentration of atherogenic remnant lipoprotein particles. (Arterioscler Thromb Vasc

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Section: Transgenic Mice Expressing Apoe In the Adrenal Glandmentioning

confidence: 99%

Low Levels of Extrahepatic Nonmacrophage ApoE Inhibit Atherosclerosis Without Correcting Hypercholesterolemia in ApoE-Deficient Mice

Thorngate

Rudel

Walzem

et al. 2000

ATVB

108

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“…The direction of the bend, in turn, may determine whether YY1 acts as an activator or repressor (30). Both parameters, DNA-bending potential (3,16,31) and strand separation potential (5) are not only functionally related but are also properties that have been associ- FIG. 10.…”

Section: Discussionmentioning

confidence: 99%

Enhanceosome Formation over the Beta Interferon Promoter Underlies a Remote-Control Mechanism Mediated by YY1 and YY2

Klar

Bode

2005

Molecular and Cellular Biology

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The expression of beta interferon genes from humans and mice is under the immediate control of a virus-responsive element (VRE) that terminates 110 bp upstream from the transcriptional start site. Whereas a wealth of information is available for the enhanceosome that is formed on the VRE upon the signals generated by viral infection, early observations indicating the existence of other far-upstream control elements have so far remained without a molecular fundament. Guided by a computational analysis of DNA structures, we could locate three as-yet-unknown transcription factor-binding regions at ؊0.5, ؊2, and ؊3 kb. Our present study delineates the interplay of factors YY1 and YY2 as it occurs at the sites at ؊3 kb and ؊2 kb (otherwise called HS1 and HS2), consistent with the idea that the novel factor YY2 antagonizes the negative actions exerted by YY1. Differences between the human and murine control regions will be described.

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“…Currently it is believed that DNA structure (perhaps a narrow minor groove, a tendency to form bent DNA, a tendency for single-strandedness and a tendency to form looped structures) is responsible for the matrix binding activity of a region (36)(37)(38)(49)(50)(51)(52). The data indicating that MARs may be enriched in inverted repeats (reviewed in 38) are of particular interest.…”

Section: Discussionmentioning

confidence: 99%

Matrix attachment regions and structural colinearity in the genomes of two grass species

1998

Current Opinion in Plant Biology

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In order to gain insights into the relationship between spatial organization of the genome and genome function we have initiated studies of the co-linear Sh2/A1-homologous regions of rice (30 kb) and sorghum (50 kb). We have identified the locations of matrix attachment regions (MARs) in these homologous chromosome segments, which could serve as anchors for individual structural units or loops. Despite the fact that the nucleotide sequences serving as MARs were not detectably conserved, the general organizational patterns of MARs relative to the neighboring genes were preserved. All identified genes were placed in individual loops that were of comparable size for homologous genes. Hence, gene composition, gene orientation, gene order and the placement of genes into structural units has been evolutionarily conserved in this region. Our analysis demonstrated that the occurrence of various 'MAR motifs' is not indicative of MAR location. However, most of the MARs discovered in the two genomic regions were found to co-localize with miniature inverted repeat transposable elements (MITEs), suggesting that MITEs preferentially insert near MARs and/or that they can serve as MARs.

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A non-curved chicken lysozyme 5' matrix attachment site is 3' followed by a strongly curved DNA sequence

Cited by 67 publications

References 33 publications

Low Levels of Extrahepatic Nonmacrophage ApoE Inhibit Atherosclerosis Without Correcting Hypercholesterolemia in ApoE-Deficient Mice

Low Levels of Extrahepatic Nonmacrophage ApoE Inhibit Atherosclerosis Without Correcting Hypercholesterolemia in ApoE-Deficient Mice

Enhanceosome Formation over the Beta Interferon Promoter Underlies a Remote-Control Mechanism Mediated by YY1 and YY2

Matrix attachment regions and structural colinearity in the genomes of two grass species

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