A non-anticoagulant heparin-like snail glycosaminoglycan promotes healing of diabetic wound

Wu, Yuebo; Zhou, Zhipeng; Li, Luo; Tao, Maixian; Chang, X.C.; Yang, Lian; Huang, Xingyong; Hu, Lin; Wu, Mingyi

doi:10.1016/j.carbpol.2020.116682

Cited by 24 publications

(17 citation statements)

References 56 publications

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“…The external application of rhEGF could promote the synthesis of DNA, RNA, and hydroxyproline during wound tissue repair and could accelerate the generation of wound granulation tissue and epithelial cell proliferation, thus shortening the wound healing time. The healing rate of the rhEGF-treated group was faster than that of gauze control group, but the wound skin tissue remodeling was worse than that of the UPH-treated group on the 11th day, which was consistent with the report of Wu et al [ 21 ]. The rhEGF-treated group mice had higher IL-6 levels on the seventh day.…”

Section: Discussionsupporting

confidence: 90%

“…In the present study, we extracted and purified UF from Saccharina japonica (Laminariaceae). Compared with some animal-derived pharmaceutical ingredients [ 20 , 21 ], the UF extracted from Laminariaceae had a broader source. A novel UPH was prepared using UF and PVA under cyclic freeze-thaw conditions.…”

Section: Discussionmentioning

confidence: 99%

“…Wu et al purified and clarified snail glycosaminoglycan (SGAG) from a cultured China white jade snail [ 21 ]. Pharmacological experiments showed that the wound healing of diabetic mice in the SGAG-H group was accelerated, and its healing curve overlapped greatly with that in the rhEGF group, which was similar to that of the UPH-treated group.…”

Section: Discussionmentioning

confidence: 99%

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Fabrication of Sulfated Heterosaccharide/Poly (Vinyl Alcohol) Hydrogel Nanocomposite for Application as Wound Healing Dressing

Liu

Geng

et al. 2022

Molecules

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Nowadays, natural polysaccharides-based hydrogels have achieved promising results as dressings to promote skin healing. In the present study, we prepared a novel hydrogel nanocomposite with poly(vinyl alcohol) (PVA) and sulfated heterosaccharide (UF), named UPH. The SEM results showed that the UPH had dense porous structures with a high porosity and a specific surface area. The UPH had a good swelling property, which can effectively adsorb exudate and keep the wound moist. The in vitro experiments results showed that the UPH was non-cytotoxic and could regulate the inflammatory response and promote the migration of fibroblasts significantly. The phenotypic, histochemistry, and Western blot analyses showed UPH treatment accelerated the wound healing and recovery of skin tissue at wound sites in a C57BL/6 mouse model. Furthermore, the UPH could promote the inflammation process to onset earlier and last shorter than that in a normal process. Given its migration-promoting ability and physicochemical properties, the UPH may provide an effective application for the treatment and management of skin wounds.

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Section: Discussionsupporting

confidence: 90%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Fabrication of Sulfated Heterosaccharide/Poly (Vinyl Alcohol) Hydrogel Nanocomposite for Application as Wound Healing Dressing

Liu

Geng

et al. 2022

Molecules

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“…Blood glucose levels ≥16.7 mM continuously for 10 days were considered characteristic of the diabetes model. Two weeks later, a full-thickness excisional skin wound was inflicted on the upper backs of the mice ( 11 , 12 ). The mice were then randomly divided into three groups with 5 mice each group: normal control group was C57BL/6J mice injected with exosomes from HUVECs transfected with ASO-NC (NC group), diabetes group was diabetic mice injected with exosomes from HUVECs transfected with ASO-NC (DM + Exo-NC), and Exo-miR-181b-5p inhibitor group was diabetic mice injected with exosomes from HUVECs transfected with miR-181b-5p inhibitor (DM + Exo-miR-181b-5p inhibitor).…”

Section: Methodsmentioning

confidence: 99%

Circulating Exosomal miR-181b-5p Promoted Cell Senescence and Inhibited Angiogenesis to Impair Diabetic Foot Ulcer via the Nuclear Factor Erythroid 2-Related Factor 2/Heme Oxygenase-1 Pathway

Wang

Shi

Zhou

et al. 2022

Front. Cardiovasc. Med.

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Endothelial cell dysfunction is the main contributing factor of diabetic foot ulcer (DFU). Circulating exosomes have been found to play an important role in many processes, such as cell senescence and angiogenesis. However, the underlying roles and mechanism of circulating exosomes in the onset and progression of DFU remain unclear. In this study, we isolated exosomes from the plasma of patients with DFU (DFU-Exos) and non-diabetic foot wounds (NDF-Exos). DFU-Exos promoted cell senescence and inhibited tube formation in Human Umbilical Vein Endothelial Cells (HUVECs), unlike NDF-Exos. Several datasets suggest that miR-181b-5p expression might be enriched in exosomes from DFU; this was verified using quantitative real-time PCR (qRT-PCR). We also found that miR-181b-5p, which was taken up by HUVECs, promoted cell senescence and inhibited tube formation. Dual luciferase reporter assay, qRT-PCR, Western blotting, and immunofluorescence staining confirmed that miR-181b-5p could negatively regulate nuclear factor erythroid 2-related factor 2 (NRF2) expression by binding to its 3′ UTR, thus further suppressing heme oxygenase-1 (HO-1) expression. In addition, NRF2 and HO-1 inhibitors could also rescue the effects of senescence and tube formation exerted by miR-181b-5p inhibitor. In vivo experiments showed that exosomes isolated from HUVECs which inhibited miR-181b-5p expression promoted angiogenesis to further restore the capacity of wound healing. In conclusion, this study indicated that circulating exosomal miR-181b-5p promoted cell senescence and inhibited angiogenesis to impair wound healing in DFU by regulating the NRF2/HO-1 pathway.

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“…However, the different extraction sources did not result in significant differences in the bioactivities favouring wound healing. Due to the difficulty of extraction, GAGs used in wound dressings are mainly of terrestrial origin [102][103][104]. In the future, finding easy extraction methods and inexpensive sources is prominent in developing marine GAGs for wound dressings [105,106].…”

Section: Marine Glycosaminoglycansmentioning

confidence: 99%

Marine Polysaccharides for Wound Dressings Application: An Overview

et al. 2021

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Wound dressings have become a crucial treatment for wound healing due to their convenience, low cost, and prolonged wound management. As cutting-edge biomaterials, marine polysaccharides are divided from most marine organisms. It possesses various bioactivities, which allowing them to be processed into various forms of wound dressings. Therefore, a comprehensive understanding of the application of marine polysaccharides in wound dressings is particularly important for the studies of wound therapy. In this review, we first introduce the wound healing process and describe the characteristics of modern commonly used dressings. Then, the properties of various marine polysaccharides and their application in wound dressing development are outlined. Finally, strategies for developing and enhancing marine polysaccharide wound dressings are described, and an outlook of these dressings is given. The diverse bioactivities of marine polysaccharides including antibacterial, anti-inflammatory, haemostatic properties, etc., providing excellent wound management and accelerate wound healing. Meanwhile, these biomaterials have higher biocompatibility and biodegradability compared to synthetic ones. On the other hand, marine polysaccharides can be combined with copolymers and active substances to prepare various forms of dressings. Among them, emerging types of dressings such as nanofibers, smart hydrogels and injectable hydrogels are at the research frontier of their development. Therefore, marine polysaccharides are essential materials in wound dressings fabrication and have a promising future.

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A non-anticoagulant heparin-like snail glycosaminoglycan promotes healing of diabetic wound

Cited by 24 publications

References 56 publications

Fabrication of Sulfated Heterosaccharide/Poly (Vinyl Alcohol) Hydrogel Nanocomposite for Application as Wound Healing Dressing

Fabrication of Sulfated Heterosaccharide/Poly (Vinyl Alcohol) Hydrogel Nanocomposite for Application as Wound Healing Dressing

Circulating Exosomal miR-181b-5p Promoted Cell Senescence and Inhibited Angiogenesis to Impair Diabetic Foot Ulcer via the Nuclear Factor Erythroid 2-Related Factor 2/Heme Oxygenase-1 Pathway

Marine Polysaccharides for Wound Dressings Application: An Overview

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