A nodule‐specific plant cysteine proteinase, AsNODF32, is involved in nodule senescence and nitrogen fixation activity of the green manure legume <i>Astragalus sinicus</i>

Li, Yixing; Zhou, Lei; Li, Youguo; Chen, Da-Song; Tan, Xuejuan; Lei, Lei; Junchu, Zhou

doi:10.1111/j.1469-8137.2008.02562.x

Cited by 50 publications

(42 citation statements)

References 27 publications

(34 reference statements)

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“…The proteins MtCP1 to MtCP6 might be specifically involved in bacteroid degradation for nutrient remobilization. Recent experiments with knockdown of the related CP gene of the Chinese milk vetch AsNODF32 support such a role (Li et al, 2008).…”

Section: Comparison Of Developmental and Dark-induced Senescence By Mmentioning

confidence: 99%

Comparison of Developmental and Stress-Induced Nodule Senescence in Medicago truncatula

et al. 2010

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Mature indeterminate Medicago truncatula nodules are zonated with an apical meristem, an infection zone, a fixation zone with nitrogen-fixing bacteroids, and a “developmental” senescence zone that follows nodule growth with a conical front originating in the center of the fixation zone. In nitrogen-fixing cells, senescence is initiated coincidently with the expression of a family of conserved cysteine proteases that might be involved in the degradation of symbiotic structures. Environmental stress, such as prolonged dark treatment, interferes with nodule functioning and triggers a fast and global nodule senescence. Developmental and dark stress-induced senescence have several different structural and expression features, suggesting at least partly divergent underlying molecular mechanisms.

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Section: Comparison Of Developmental and Dark-induced Senescence By Mmentioning

confidence: 99%

Comparison of Developmental and Stress-Induced Nodule Senescence in Medicago truncatula

et al. 2010

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“…For GigmPT silencing by the host, 305 bp of the GigmPT cDNA at 5′ end was cloned using the specific primers GigmPTdsF1 and GigmPTdsR1 and introduced into the RNAi vector pDS1301 ( Chu et al, 2006). The GigmPT RNAi construct was used to generate A. sinicus hairy roots by Agrobacterium rhizogenes transformation as described by Li et al (2008). The EV control and RNAi A. sinicus hairy roots were subsequently inoculated with G. margarita and the colonization was detected at 28 days post inoculation (dpi).…”

Section: Host-induced Gene Silencingmentioning

confidence: 99%

Arbuscular Mycorrhizal Symbiosis Requires a Phosphate Transceptor in the Gigaspora margarita Fungal Symbiont

et al. 2016

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The definitive version is available at: La versione definitiva è disponibile alla URL: [http://www.sciencedirect.com AbstractThe majority of terrestrial vascular plants are capable of forming mutualistic associations with obligate biotrophic arbuscular mycorrhizal (AM) fungi from the phylum Glomeromycota. This mutualistic symbiosis provides carbohydrates to the fungus, and reciprocally improves plant phosphate uptake. AM fungal transporters can acquire phosphate from the soil through the hyphal networks. Nevertheless, the precise functions of AM fungal phosphate transporters, and whether they act as sensors or as nutrient transporters, in fungal signal transduction remain unclear. Here, we report a high-affinity phosphate transporter GigmPT from Gigaspora margarita that is required for AM symbiosis. Host-induced gene silencing of GigmPT hampers the development of G. margarita during AM symbiosis. Most importantly, GigmPT functions as a phosphate transceptor in G. margarita regarding the activation of the phosphate signaling pathway as well as the protein kinase A signaling cascade. Using the substituted-cysteine accessibility method, we identified residues A146 (in transmembrane domain [TMD] IV) and Val357 (in TMD VIII) of GigmPT, both of which are critical for phosphate signaling and transport in yeast during growth induction. Collectively, our results provide significant insights into the molecular functions of a phosphate transceptor from the AM fungus G. margarita.

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“…As the nodule ages, this zone gradually moves in a proximal-distal direction until it reaches the apical part and the nodule degenerates (Monahan-Giovanelli et al, 2006;Van de Velde et al, 2006). Chinese milk vetch is mainly distributed in China, Japan, and Korea, where it is widely planted in rice fields to increase soil fertility (Li et al, 2008).…”

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confidence: 99%

A Nodule-Specific Lipid Transfer Protein AsE246 Participates in Transport of Plant-Synthesized Lipids to Symbiosome Membrane and Is Essential for Nodule Organogenesis in Chinese Milk Vetch

et al. 2013

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Rhizobia in legume root nodules fix nitrogen in symbiosomes, organelle-like structures in which a membrane from the host plant surrounds the symbiotic bacteria. However, the components that transport plant-synthesized lipids to the symbiosome membrane remain unknown. This study identified and functionally characterized the Chinese milk vetch (Astragalus sinicus) lipid transfer protein AsE246, which is specifically expressed in nodules. It was found that AsE246 can bind lipids in vitro. More importantly, AsE246 can bind the plant-synthesized membrane lipid digalactosyldiacylglycerol in vivo. Immunofluorescence and immunoelectron microscopy showed that AsE246 and digalactosyldiacylglycerol localize in the symbiosome membrane and are present in infection threads. Overexpression of AsE246 resulted in increased nodule numbers; knockdown of AsE246 resulted in reduced nodule numbers, decreased lipids contents in nodules, diminished nitrogen fixation activity, and abnormal development of symbiosomes. AsE246 knockdown also resulted in fewer infection threads, nodule primordia, and nodules, while AsE246 overexpression resulted in more infection threads and nodule primordia, suggesting that AsE246 affects nodule organogenesis associated with infection thread formation. Taken together, these results indicate that AsE246 contributes to lipids transport to the symbiosome membrane, and this transport is required for effective legume-rhizobium symbiosis.

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A nodule‐specific plant cysteine proteinase, AsNODF32, is involved in nodule senescence and nitrogen fixation activity of the green manure legume Astragalus sinicus

Cited by 50 publications

References 27 publications

Comparison of Developmental and Stress-Induced Nodule Senescence in Medicago truncatula

Comparison of Developmental and Stress-Induced Nodule Senescence in Medicago truncatula

Arbuscular Mycorrhizal Symbiosis Requires a Phosphate Transceptor in the Gigaspora margarita Fungal Symbiont

A Nodule-Specific Lipid Transfer Protein AsE246 Participates in Transport of Plant-Synthesized Lipids to Symbiosome Membrane and Is Essential for Nodule Organogenesis in Chinese Milk Vetch

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