A NMR study of parasitized Tenebrio molitor and Hymenolepis diminuta cysticercoids

Schoen, Jodi M.; Modha, A.; Maslow, K.; Novak, Marie; Blackburn, B. J.

doi:10.1016/0020-7519(96)00050-1

Cited by 7 publications

(7 citation statements)

References 22 publications

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“…Studies on nutrient pathways in infected hosts have used the much larger T. molitor . These have shown that cysticercoids do not utilize trehalose, the most abundant sugar in the haemolymph (Pappas and Morrison, 1995) and infected T. molitor may actually have increased levels of trehalose, various amino acids and vitellogenin in the haemolymph but no change in glucose concentrations (Hurd and Arme, 1984 a , b ; Kearns et al 1994; Schoen et al 1996). Use of glucose by developing H. diminuta is probably a combination of direct utilization of products of digestion en route to the fat body and altered mobilization of existing host reserves (Kearns et al 1994).…”

Section: Discussionmentioning

confidence: 99%

Manipulation of host food availability and use of multiple exposures to assess the crowding effect onHymenolepis diminutainTribolium confusum

2008

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We studied the 'crowding effect' in Tribolium confusum infected with Hymenolepis diminuta. Manipulations included age and number of parasites, and diet, sex, age and number of exposures of hosts. Volume per parasite was unaffected until an intensity of at least 5-10 parasites per host, then declined approximately inversely as intensities increased. Parasite size was affected by host sex but not age or reproductive status. Host diet affected parasite size and the impact of crowding. Daily gain in parasite volume peaked partway through the developmental period and preceded the first evidence of a crowding effect. Parasites that established during a second exposure had a transient developmental delay but eventually grew as large or larger than parasites from a single exposure with the same total intensity. Parasites responded to crowding by differential allocation of resources. Cercomer volume decreased even with slight crowding, the capsule surrounding the scolex was not reduced until crowding became more severe, and scolex width was reduced only in the most extreme conditions. The data support the hypothesis that the crowding effect in this system is driven primarily by nutrient, rather than space limitations.

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Section: Discussionmentioning

confidence: 99%

Manipulation of host food availability and use of multiple exposures to assess the crowding effect onHymenolepis diminutainTribolium confusum

2008

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“…77,107 1 H NMR is a useful tool for metabolic profiling and monitoring metabolic changes in natural sources. Schoen et al 108 proposed a quantitative metabolic profiling assay based on NMR of perchloric acid extracts of Hymenolopsis diminuta (cysticercoids) parasitized Tenebrio molitor beetles. Infected beetles possessed less glycerophosphocholine but more glycogen and a higher percentage label in glucose and trehalose than their respective controls.…”

Section: Applications Of Qhnmrmentioning

confidence: 99%

“…Quantities of metabolites present and the percent 13 C enrichment were calculated from integration values of the metabolite peaks relative to that of TSP. 108 The metabolism the tomato saponin R-tomatine (1) to the aglycon tomatidine, and further to 7R-hydroxytomatidine and the minor corresponding ∆ 5 -dehydro product 7Rhydroxydehydrotomatidenol, by Gibberella pulicaris, was published by Weltring et al 99 For structure elucidation, the major metabolites were isolated, and the purity and the content of the minor dehydro metabolic side product (20%) were evaluated by qHNMR, measured at 600 MHz. High-resolution qHNMR has turned out to be a promising screening technique, which could answer some of the concerns caused by genetically modified organisms.…”

Section: Applications Of Qhnmrmentioning

confidence: 99%

Quantitative ¹H NMR: Development and Potential of a Method for Natural Products Analysis

2004

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Based on a brief revision of what constitutes state-of-the-art "quantitative experimental conditions" for (1)H quantitative NMR (qHNMR), this comprehensive review contains almost 200 references and covers the literature since 1982 with emphasis on natural products. It provides an overview of the background and applications of qHNMR in natural products research, new methods such as decoupling and hyphenation, and analytical potential and limitations, and compiles information on reference materials used for and studied by qHNMR. The dual status of natural products, being single chemical entities and valuable biologically active agents that need to be purified from complex matrixes, results in an increased analytical demand when testing their deviation from the singleton composition ideal. The outcome and versatility of reported applications lead to the conclusion that qHNMR is currently the principal analytical method to meet this demand. Considering both 1D and 2D (1)H NMR experiments, qHNMR has proved to be highly suitable for the simultaneous selective recognition and quantitative determination of metabolites in complex biological matrixes. This is manifested by the prior publication of over 80 reports on applications involving the quantitation of single natural products in plant extracts, dietary materials, and materials representing different metabolic stages of (micro)organisms. In summary, qHNMR has great potential as an analytical tool in both the discovery of new bioactive natural products and the field of metabolome analysis.

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“…The cysts grown in the abdominal cavity were found to contain lower concentrations of glucose and phosphocreatine but more succinate, acetate, alanine and 3-D-hydroxybutyrate than subcutaneous cysts. A similar study used D-(1-13C)glucose with 13 C NMR monitoring to investigate metabolism in Hymenolepis diminuta -infected and uninfected Tenebrio molitor beetles (Schoen et al 1996). Infected beetles contained less glycerophosphocholine and more glycogen than controls.…”

Section: Profiling Of the Host Metabolic Statusmentioning

confidence: 99%

The evolution of metabolic profiling in parasitology

Holmes

2010

Parasitology

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The uses of metabolic profiling technologies such as mass spectrometry and nuclear magnetic resonance spectroscopy in parasitology have been multi-faceted. Traditional uses of spectroscopic platforms focused on determining the chemical composition of drugs or natural products used for treatment of parasitic infection. A natural progression of the use of these tools led to the generation of chemical profiles of the parasite in in vitro systems, monitoring the response of the parasite to chemotherapeutics, profiling metabolic consequences in the host organism and to deriving host-parasite interactions. With the dawn of the post-genomic era the paradigm in many research areas shifted towards Systems Biology and the integration of biomolecular interactions at the level of the gene, protein and metabolite. Although these technologies have yet to deliver their full potential, metabolic profiling has a key role to play in defining diagnostic or even prognostic metabolic signatures of parasitic infection and in deciphering the molecular mechanisms underpinning the development of parasite-induced pathologies. The strengths and weaknesses of the various spectroscopic technologies and analytical strategies are summarized here with respect to achieving these goals.

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A NMR study of parasitized Tenebrio molitor and Hymenolepis diminuta cysticercoids

Cited by 7 publications

References 22 publications

Manipulation of host food availability and use of multiple exposures to assess the crowding effect onHymenolepis diminutainTribolium confusum

Manipulation of host food availability and use of multiple exposures to assess the crowding effect onHymenolepis diminutainTribolium confusum

Quantitative ¹H NMR: Development and Potential of a Method for Natural Products Analysis

The evolution of metabolic profiling in parasitology

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A NMR study of parasitized Tenebrio molitor and Hymenolepis diminuta cysticercoids

Cited by 7 publications

References 22 publications

Manipulation of host food availability and use of multiple exposures to assess the crowding effect onHymenolepis diminutainTribolium confusum

Manipulation of host food availability and use of multiple exposures to assess the crowding effect onHymenolepis diminutainTribolium confusum

Quantitative 1H NMR: Development and Potential of a Method for Natural Products Analysis

The evolution of metabolic profiling in parasitology

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Quantitative ¹H NMR: Development and Potential of a Method for Natural Products Analysis