A NIR-II fluorescent probe for articular cartilage degeneration imaging and osteoarthritis detection

Yi, Wanrong; Zhou, Hui; Li, Anguo; Yuan, Ying; Guo, Yongli; Li, Pengcheng; Qi, Baiwen; Xiao, Yuling; Yu, Aixi; Hu, Xiang

doi:10.1039/c8bm01440j

Cited by 40 publications

(34 citation statements)

References 37 publications

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“…NIR-II probes CH1055-GK and CH1055-PEG (as a control) were synthesized with CH1055 and the peptide GRRRDEVDK or its control COtBu-PEG4-NH2 (Chart 1 and Figure S1). As reported in our previous work, the NIR absorption bands of CH1055-WYRGRL (CH1055-WL) exhibited high stability and photostability, which would allow the probe to emit stable fluorescence in living organisms for a long time (Yi et al, 2019). Similarly, in this study, we designed the CH1055-GK probe, and the MALDI-TOF-MS measurement result for CH1055-GK was 2.064 KDa, while the expected M.W.…”

Section: Resultsmentioning

confidence: 77%

NIR-II Fluorescence Imaging of Skin Avulsion and Necrosis

et al. 2019

Front. Chem.

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Skin avulsion is commonly seen in individuals exposed to heavy shearing forces. Subcutaneous tissue detachment and bone fractures usually accompany skin avulsion. Thus, the estimation of the extent of damaged tissue is very important. Currently, the viability of skin and subcutaneous tissue is determined by clinical observations, and these observations always underestimate the true extent of the avulsed skin. Herein, we synthesized an innovative probe, CH1055-GRRRDEVDK (CH1055-GK), which can specifically bind to caspase-3 so as to image skin avulsion and define necrotic regions. Our uptake and binding affinity tests in apoptotic cells and evaluation of the probe ex vivo and in vivo showed that the probe has a strong ability to bind caspase-3 in skin avulsion models and that it vividly detected the necrotic area in avulsed skins. Furthermore, the increased fluorescence intensity of the probe in the avulsed skin showed a larger affected area than that determined by clinical observations in live mice. Consequently, our results indicated that observation of the caspase-3-targeted probe CH1055-GK via NIR-II imaging allowed the clear detection of skin avulsion in subjects, indicating its potential as an imaging tool for the early diagnosis of skin avulsion and the determination of necrotic margins.

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Section: Resultsmentioning

confidence: 77%

NIR-II Fluorescence Imaging of Skin Avulsion and Necrosis

et al. 2019

Front. Chem.

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“…With excellent hydrophilic ability, PEG chains grafted on nanoparticle generate a su ciently thick hydrated cloud [35] that strongly prevents NPs from aggregation. Functionalized with low molecular weight PEG, nanomaterials have enhanced penetration ability through mucus [36] and other tissues, which facilitated drug delivery to the lung [37], breast [38] and colorectal carcinomas [39]. CollB-peptide has been demonstrated to bind to collagen II α1 and shows outstanding application in drug delivery and imaging [28,40].…”

Section: Discussionmentioning

confidence: 99%

Cartilage-targeting Poly(Ethyleneglycol) (PEG)-formononetin (FMN) Nanodrug for Treating Osteoarthritis

Wang

Lan

Liang

et al. 2021

Preprint

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Intra-articular (IA) injection is an efficient treatment for osteoarthritis. Such a treatment will minimize systemic side effects and avoid the inconvenience of frequent injections. However, the joint has poor bioavailability for systemically administered drugs and experiences rapid clearance of therapeutics after intra-articular injection. Delivering system modified through active targeting strategies to facilitate localization within specific joint tissues such as cartilage is hopeful to increase the therapeutic effects. In this study, we designed a nanoscaled amphiphilic and cartilage-targeting polymer-drug delivery system by using formononetin (FMN)-poly(ethylene glycol) (PEG) (denoted as PCFMN), which was prepared by PEGylation of FMN followed by coupling with cartilage-targeting peptide (CollBP). Our results showed that PCFMN was approximately regular spherical with the average diameter about 218 nm. The in vitro test using IL-1β stimulated chondrocytes indicated that PCFMN were biocompatible and upregulated anabolic genes while simultaneously downregulated catabolic genes of the articular cartilage. The therapeutic effects in vivo indicated that PCFMN could effectively attenuate the progression of OA as evidenced by immunohistochemical staining and histological analysis. In addition, PCFMN showed higher intention time in joints and better anti-inflammatory effects than FMN, indicating the efficacy of cartilage targeting nanodrug on OA. This study may provide reference for clinical OA therapy.

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“…Functionalization of poly(propylene sulphide nanoparticles and subsequent IA injection in mice knees resulted in a 72‐fold increase in cartilage‐targeting ability compared to nanoparticles functionalized with the scrambled control 103 . In contrast to other reported peptides, this sequence has been successfully used in preclinical models for diagnostics and drug delivery applications 12,91,103‐106 . In fact, the conjugation of this peptide to magnetic resonance imaging (MRI) contrast agents has allowed in vivo localization of cartilage hypertrophic changes in a rat model of OA 104 .…”

Section: Targeting For Ia Drug Deliverymentioning

confidence: 99%

Biomaterial strategies for improved intra‐articular drug delivery

Castro

Garcı́a

Guldberg

2020

J Biomedical Materials Res

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Osteoarthritis (OA) is a joint degenerative disease that has become one of the leading causes of disability in the world. It is estimated that OA affects 50 million adults in the United States. Currently, there are no FDA‐approved treatments that slow OA progression and its treatment is limited to pain management strategies and life style changes. Despite the discovery of several disease‐modifying OA drugs (DMOADs) and promising results in preclinical studies, their clinical translation has been significantly limited because of poor intra‐articular (IA) bioavailability and challenges in delivering these compounds to tissues of interest within the joint. Here, we review current OA treatments and their effectiveness at reducing joint pain, as well as novel targets for OA treatment and the challenges related to their clinical translation. Moreover, we discuss intra‐articular (IA) drug delivery as a promising route of administration, describe its inherent challenges, and review recent advances in biomaterial‐based IA drug delivery for OA treatment. Finally, we highlight the potential of tissue targeting in the development of effective IA drug delivery systems.

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A NIR-II fluorescent probe for articular cartilage degeneration imaging and osteoarthritis detection

Abstract: A Type II collagen-binding peptide based NIR-II fluorescent probe for articular cartilage degeneration imaging and early osteoarthritis detection.

Cited by 40 publications

References 37 publications

NIR-II Fluorescence Imaging of Skin Avulsion and Necrosis

NIR-II Fluorescence Imaging of Skin Avulsion and Necrosis

Cartilage-targeting Poly(Ethyleneglycol) (PEG)-formononetin (FMN) Nanodrug for Treating Osteoarthritis

Biomaterial strategies for improved intra‐articular drug delivery

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