A Nicotinamide Adenine Dinucleotide Dispersed Multi-walled Carbon Nanotubes Electrode for Direct and Selective Electrochemical Detection of Uric Acid

Abstract

+ has an adenine subunit and a nicotinamide subunit, which can absorb MWCNTs through the π-stacking interaction, 24 A nanocomposite platform built with multi-walled carbon nanotubes (MWCNTs) and nicotinamide adenine dinucleotide (NAD + ) via a noncovalent interaction between the large π systems in NAD + molecules and MWCNTs on a glassy carbon substrate was successfully developed for the sensitive and selective detection of uric acid (UA) in the presence of ascorbic acid (AA), dopamine (DA). NAD + has an adenine subunit and a nicotinamide subunit, which enabled interaction with the purine subunit of UA through a strong π-π interaction to enhance the specificity of UA. Compared with a bare glassy carbon electrode (GCE) and MWCNTs/GCE, the MWCNTs-NAD + /GCE showed a low background current and a remarkable enhancement of the oxidation peak current of UA. Using differential pulse voltammetry (DPV), a high sensitivity for the determination of UA was explored for the MWCNTs-NAD + modified electrode. A linear relationship between the DPV peak current of UA and its concentration could be obtained in the range of 0.05 -10 μM with the detection limit as low as 10 nM (S/N = 3). This present strategy provides a novel and promising platform for the detection of UA in human urine and serum samples.

Development and Application of a Voltammetric Biosensor Based on Polypyrrole/uricase/graphene for Uric Acid Determination

“…However, problems such as the adsorption of oxidation/reduction products on the electrode surface and also the oxidation in values of high potential lead to instability of the analytical signal. To overcome these difficulties, various forms of electrode modification have emerged to use in determining UA, and some examples of commonly used materials are enzymes (UOx) and biomimetic catalysts[6a], reduced graphene oxide on glassy carbon electrode , UOx immobilized on functionalized gold coated copper wire electrode , polypyrrole films doped with ferrocyanide ions onto iron substrate , reduced graphene oxide and ZnO nanorods‐modified glassy carbon electrode , a nicotinamide adenine dinucleotide dispersed multi‐walled carbon nanotubes electrode , chitosan‐graphene modified electrode , among others.…”