A next generation, pilot-scale continuous sterilization system for fermentation media

Abstract

A new continuous sterilization system was designed, constructed, started up, and qualified for media sterilization for secondary metabolite cultivations, bioconversions, and enzyme production. An existing Honeywell Total Distributed Control 3000-based control system was extended using redundant High performance Process Manager controllers for 98 I/O (input/output) points. This new equipment was retrofitted into an industrial research fermentation pilot plant, designed and constructed in the early 1980s. Design strategies of this new continuous sterilizer system and the expanded control system are described and compared with the literature (including dairy and biowaste inactivation applications) and the weaknesses of the prior installation for expected effectiveness. In addition, the reasoning behind selection of some of these improved features has been incorporated. Examples of enhancements adopted include sanitary heat exchanger (HEX) design, incorporation of a ''flash'' cooling HEX, on-line calculation of F o and R o , and use of field I/O modules located near the vessel to permit low-cost addition of new instrumentation. Sterilizer performance also was characterized over the expected range of operating conditions. Differences between design and observed temperature, pressure, and other profiles were quantified and investigated. Reynolds number, DVq/g P system back-pressure, kg f /cm 2 Q system volumetric flowrate, lpm Q DT Q-value, death rate increase for specified change in temperature, DT Q M system mass flow rate, kg/s R universal gas constant, 1.987 cal/mol-K R o time at specified sterilization hold temperature, T, equivalent to exposure to a saturated steam environment of 121°C for nutrient inactivation, min

The effect of organic acid type on thermal inactivation of Geobacillus stearothermophilus spores

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Physicochemical and Fatty Acid Profile of Fish Oil from Head of Tuna (Thunnus albacares) Extracted from Various Extraction Method

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Nanoparticles Biosynthesized by Yeast: A Review of their application

“…With the mass production of fermented products, the requirements on sterile environment have been occurred and the protection against pathogenic microorganisms (Junker et al, 2006;Zhao et al, 2015).…”