A newly thermoactive and detergent‐stable lipase from annular sea bream (<i>Diplodus annularis</i>): Biochemical properties

Smichi, Nabil; Miled, Nabil; Gargouri, Youssef; Fendri, Ahmed

doi:10.1002/bab.1445

Cited by 6 publications

(11 citation statements)

References 59 publications

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“…Interestingly, the fish enzyme preserved 95% of its maximal activity in the presence of high concentrations of NaDC. Similar results were previously obtained with purified lipase from the annular seabream and other digestive lipases isolated from sardine and crab . Furthermore, this behavior is different from that of stingray pancreatic lipase, which showed an absolute requirement for bile salts .…”

Section: Resultssupporting

confidence: 87%

“…The enzyme apparent molecular mass is about 50 kDa. This is in line with the molecular mass of AsDL .…”

Section: Resultssupporting

confidence: 84%

“…The optimal activity of the purified lipase was recorded at pH 9 and at 50°C. Thus, the RsDL, like AsDL , is more thermoactive than most of fish digestive lipases, previously characterized, which display a maximal activity around 40°C . The optimum pH value for the RsDL activity was close to that reported for digestive lipolytic enzymes from other fish species such as sardine and golden grey mullet .…”

Section: Resultssupporting

confidence: 72%

“…The specific activities of RsDL are 20, 10, and 4 U/mg on TC 4 , TC 8 , or olive oil, respectively, measured at pH 9 and 50°C (Table ). Although these values are lower than those obtained with AsDL and the dromedary pancreatic lipase (a mammal model) (Table ), the RsDL displays the same tendency of most of known digestive lipases to hydrolyze short‐chain triacylglycerols more efficiently than medium and long chain ones. Comparable results were obtained for sardine (SaDL) and golden grey mullet (GmDL) digestive lipases.…”

Section: Resultsmentioning

confidence: 58%

“…However, little information is available concerning structure–function relationships for sparidae digestive lipases. Recently, we have purified and characterized a digestive lipase (AsDL) from a sparidae fish, the annular seabream . This lipase shows different properties with classical lipases.…”

Section: Introductionmentioning

confidence: 99%

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Biochemical characterization, cloning and molecular modeling of a digestive lipase from red seabream (Pagrus major): Structural explanation of the interaction deficiency with colipase and lipidic interface

Smichi¹,

Fendri²,

Triki-Fendri

et al. 2017

Engineering in Life Sciences

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Biochemical characterization, cloning and molecular modeling of a digestive lipase from red seabream (Pagrus major): Structural explanation of the interaction deficiency with colipase and lipidic interfaceRed seabream digestive lipase (RsDL) was purified from fresh pyloric caeca. Pure RsDL has an apparent molecular mass of 50 kDa. The RsDL is more active on shortchain triacylglycerols (TC 4 ), and enzymatic activity decreases when medium (TC 8 ) or long-chain (olive oil) triacylglycerols were used as substrates. The specific activities of RsDL are very weak as compared to those obtained with classical pancreatic lipases. No colipase was detected in the red seabream pyloric caeca. Furthermore, the RsDL was not activated by a mammal colipase. Similar results were reported for annular seabream lipase. In order to explain structurally the discrepancies between sparidae and mammal lipases, genes encoding mature RsDL and five other lipases from sparidae fish species were cloned and sequenced. Phylogenetic studies indicated the closest homology of sparidae lipases to bird pancreatic ones. Structural models were built for annular seabream and RsDL under their closed and open forms using mammal pancreatic lipases as templates. Several differences were noticed when analyzing the amino acids corresponding to those involved in HPL binding to colipase. This is likely to prevent interaction between the fish lipase and the mammalian colipase and may explain the fact that mammalian colipase is not effective in activating sparidae lipases. In addition, several hydrophobic residues, playing a key role in anchoring pancreatic lipase onto the lipid interface, are replaced by polar residues in fish lipases. This might explain the reason why the latter enzymes display weak activity levels when compared to mammalian pancreatic lipases.Abbreviations: AsDL, annular seabream digestive lipase; DL, digestive lipase; DPLRP1, dog pancreatic lipase related protein 1; GhDL, gilthead seabream digestive lipase; HPL, human pancreatic lipase; HPLRP1, human pancreatic lipase related protein 1; HPLRP2, human pancreatic lipase related protein 2; PCR, polymerase chain reaction; PL, pancreatic lipase; PPL, porcine pancreatic lipase; PPLRP2, porcine pancreatic lipase related protein 2; RP, related pancreatic lipase; RsDL, red seabream digestive lipase; RT, reverse transcription; SpDL, Saupe digestive lipase; SsDL, striped seabream digestive lipase; TPL, turkey pancreatic lipase; WsDL, white seabream digestive lipase

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Section: Resultssupporting

confidence: 87%

“…The enzyme apparent molecular mass is about 50 kDa. This is in line with the molecular mass of AsDL .…”

Section: Resultssupporting

confidence: 84%

Section: Resultssupporting

confidence: 72%

Section: Resultsmentioning

confidence: 58%

Section: Introductionmentioning

confidence: 99%

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Biochemical characterization, cloning and molecular modeling of a digestive lipase from red seabream (Pagrus major): Structural explanation of the interaction deficiency with colipase and lipidic interface

Smichi¹,

Fendri²,

Triki-Fendri

et al. 2017

Engineering in Life Sciences

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Byproducts from Fish Harvesting and Processing

Benjakul¹,

Sae‐leaw²,

Simpson³

2019

Byproducts From Agriculture and Fisheries

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Assessment of the effects of sex and harvesting season on lipid and fatty acid composition of Sparidae species

Metin

Alparslan

Yapıcı

et al. 2021

Lipids

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In the present study, the lipid amount and fatty acid profile of different Sparidae species, including gilthead seabream, juvenile gilthead seabream, annular seabream, white seabream, common two-banded seabream were evaluated. Fish were seasonally collected from Köyce giz Lagoon (Mu gla, South Western Turkey) from June 2018 to June 2019 and after collection, the sex of each specimen was recorded. According to the results of the study, the highest lipid amount was found in female annular seabream individuals as 8.09 AE 0.78% in November and the lowest lipid amount was found in male juvenile gilthead seabream as 0.98 AE 0.12% in March. Palmitic acid and oleic acid were determined as the most abundant SFA and MUFA for all species, respectively. The highest value of DHA, which was the predominant PUFA was assessed as 15.33 AE 0.26% in female white seabream in November whereas the lowest value (3.83 AE 0.36%) was found in gilthead seabream in December. The n-6/n-3 ratio was determined between 0.27 AE 0.00 (for male common two-banded seabream in July)-1.20 AE 0.03 (for male gilthead seabream in December) and it followed within the range of healthy values for all species. As a conclusion, it was found that values of lipid and fatty acid profiles among the examined Sparidae species vary among the season of collection. The results of the study gave the seasonal nutritional values of four economically-important Sparidae species that being rich in healthy polyunsaturated fatty acids such as DHA, have beneficial in human nutrition. Keywords Fatty acids Á GLC (GC) (gas-liquid chromatography) Á Lipid analysis Á MUFA Á N-3 fatty acids Lipids (2021) 56: 391-404. Abbreviations MUFA monounsaturated fatty acids n omega PUFA polyunsaturated fatty acids SFA saturated fatty acids * Cansu Metin

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A newly thermoactive and detergent‐stable lipase from annular sea bream (Diplodus annularis): Biochemical properties

Cited by 6 publications

References 59 publications

Biochemical characterization, cloning and molecular modeling of a digestive lipase from red seabream (Pagrus major): Structural explanation of the interaction deficiency with colipase and lipidic interface

Biochemical characterization, cloning and molecular modeling of a digestive lipase from red seabream (Pagrus major): Structural explanation of the interaction deficiency with colipase and lipidic interface

Byproducts from Fish Harvesting and Processing

Assessment of the effects of sex and harvesting season on lipid and fatty acid composition of Sparidae species

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