A Newly-Recognized Enterovirus Isolated From Cases of Aseptic Meningitis1

Plager, Hildegard; Decher, W

doi:10.1093/oxfordjournals.aje.a120292

Cited by 13 publications

(7 citation statements)

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“…Propagation of the isolates was performed in MRC5 cells by previously described methods (3) and was limited to a maximum of two passages. The EV30 prototype strain, EV30/1958/USA/Bastianni (32), and two recent isolates (91CF670 and 98CF746) collected from patients with aseptic meningitis were included in this study as controls for the phylogenetic analyses.…”

Section: Methodsmentioning

confidence: 99%

Genomic Variations in Echovirus 30 Persistent Isolates Recovered from a Chronically Infected Immunodeficient Child and Comparison with the Reference Strain

et al. 2000

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Seven sequential isolates of echovirus type 30 (EV30) were recovered over 22 months from a child with severe combined immune deficiency syndrome. The nucleotide sequences of the 5′ halves of the genomes (4,400 nucleotides) of the first (S1) and last (S7) isolates were determined and compared with that of the EV30 Bastianni reference strain, also determined in this study. In genome regions P1 and P2, 101 variations were identified between the two isolates. Synonymous differences far outnumbered nonsynonymous differences. Amino acid changes affected both capsid and nonstructural polypeptides (particularly 2B). The VP1 nucleotide sequences of the seven isolates were determined to analyze genome evolution during the chronic infection. In the phylogenetic tree, the seven isolates were directly related to the prototype strain in an individual monophyletic group, strongly suggesting that the chronic infection in the child arose from a single persistent EV30 isolate. Four lineages were observed in the persistent isolates. Isolates S2, S4, S5, and S6 were close relatives of one another, whereas isolates S1 and S3 formed individual lineages. Isolate S7, distantly related to all other isolates, formed the fourth lineage. These findings suggest the quasispecies nature of the genomes of the seven sequential EV30 isolates. Grouping of persistent isolates on the basis of replicative capacities was consistent with phylogenetic relationships. Overall, the results indicate that genetically related EV30 variants with different replicative capacities coexisted in a carrier state, probably in the gastrointestinal tract, during the infection of the child.

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Section: Methodsmentioning

confidence: 99%

Genomic Variations in Echovirus 30 Persistent Isolates Recovered from a Chronically Infected Immunodeficient Child and Comparison with the Reference Strain

et al. 2000

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“…Among the enteroviruses, the echoviruses, particularly echovirus 30, are implicated most frequently in viral meningitis [Atkinson et al, 1998]. The role of echovirus 30 in the etiology of this disease has been known since the virus was first isolated in 1958 [Plager and Dechler, 1963]. The distribution of recent meningitis outbreaks caused by echovirus 30 strongly suggests that infections are widespread [Leonardi et al, 1993;Drebot et al, 1994;Rice et al, 1995;Lopez-Alcala et al, 1997;Gorgievski-Hrisoho et al, 1998;Khalfan et al, 1998;Reintjes et al, 1999;Uysal et al, 2000].…”

Section: Introductionmentioning

confidence: 99%

Genetic diversity of echovirus 30 during a meningitis outbreak, demonstrated by direct molecular typing from cerebrospinal fluid

Bailly

Brosson

Archimbaud

et al. 2002

Journal of Medical Virology

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Echovirus 30 is one of the enterovirus serotypes isolated most frequently in meningitis cases. The genetic diversity of echovirus 30 was investigated in patients hospitalised during an outbreak in 2000 in Clermont-Ferrand, France. A nested reverse transcription-PCR (RT-PCR) assay was developed for qualitative analysis of the echovirus 30 VP1 encoding sequence directly from cerebrospinal fluid. The viral sequences obtained for 22 patients were compared with those of virus isolates obtained from nine patients with echovirus 30 meningitis admitted to hospital in 1996-1997 and with echovirus 30 sequences from international databases. In 2000, meningitis cases were caused by two virus variants (C3 and C4) distinct genetically from the other two variants (C1 and C2) identified during the period 1996-1997. A detailed phylogenetic analysis established that the C1, C2, and C3 variants had close relatives among viruses previously identified in other geographical areas. The C4 variant had not been described earlier. The genomic differences observed between the four echovirus 30 variants arose at synonymous sites indicating that the viruses shared similar antigenic sites in the VP1 encoding sequence. Overall, these observations suggest wide circulation of different echovirus 30 variants and periodic importation of new viruses. The apparent displacement observed between virus variants did not result from a selective advantage caused by antigenic variation.

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“…VP1 nucleotide sequence alignment showed that the nine isolates of E13 had high nucleotide divergence (23.1–24.0%) with the prototype strain, Del Carmen (AY302539) which was isolated from 4-month-old female in Philippine Islands, 195317. E30 had 15.8–16.6% nucleotide divergence with the prototype strain Bastianni (AF162711) which was isolated from a patient suffered from aseptic meningitis in New York, 195818. E14 could be detected with 1–2 strains every year during 2006–2009, but the 5 strains exhibited high nucleotide divergence ranging from 6.8–17.7%.…”

Section: Resultsmentioning

confidence: 99%

Molecular Epidemiology and Recombination of Human Enteroviruses from AFP surveillance in Yunnan, China from 2006 to 2010

Tang

Yoshida

Ding

et al. 2014

Sci Rep

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The study represents the genetic overview of non-polio enteroviruses (NPEV) isolated from acute flaccid paralysis (AFP) cases in Yunnan Province from 2006 to 2010. Molecular typing based on VP1 nucleotide sequence was carried out on 98 NPEV isolates, and 33 serotypes were identified. EV-B was detected most frequently with an overall prevalence of 71.4%, followed by EV-A (18.4%) and EV-C (10.2%). No EV-D was identified. NPEV positive rate was higher in children <3 years of age and in summer and autumn months. Clinically, 68.4% patients presented with fever, and 16 cases (16.3%) were classified as Guillain-Barré syndrome, followed by myositis (13.3%). The phylogenetic analysis on the VP1 and 3D regions of prevalent serotypes provided evidence for recombination events among them. EV-A71, an important pathogen previously demonstrated to be associated with paralysis, had also been detected (n = 8) in this study and they all belonged to genotype C4. Great genetic divergence between Yunnan isolates and strains from other regions of the world was revealed. The findings of the study are of great importance for further research on molecular evolution of EV under the circumstance of no specialized EV surveillance system in China.

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A Newly-Recognized Enterovirus Isolated From Cases of Aseptic Meningitis1

Cited by 13 publications

References 0 publications

Genomic Variations in Echovirus 30 Persistent Isolates Recovered from a Chronically Infected Immunodeficient Child and Comparison with the Reference Strain

Genomic Variations in Echovirus 30 Persistent Isolates Recovered from a Chronically Infected Immunodeficient Child and Comparison with the Reference Strain

Genetic diversity of echovirus 30 during a meningitis outbreak, demonstrated by direct molecular typing from cerebrospinal fluid

Molecular Epidemiology and Recombination of Human Enteroviruses from AFP surveillance in Yunnan, China from 2006 to 2010

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