A newly recognised Australian endemic species of Austrolecanium Gullan & Hodgson 1998 (Hemiptera: Coccidae) from Queensland

Abstract

Austrolecanium cryptocaryae Lin & Cook sp. n. is described based on adult female morphology and DNA sequences from mitochondrial and nuclear loci. This Australian endemic species was found on the underside of leaves of Cryptocarya microneura (Lauraceae) in Queensland. All phylogenetic analyses of four independent DNA loci and a concatenated dataset show that A. cryptocaryae is monophyletic and closely related to A. sassafras Gullan & Hodgson, the type species of Austrolecanium Gullan & Hodgson. The adult female of A. cryptocaryae is described and illustrated and a table is provided of the characters that differ among adult females of the three species of Austrolecanium currently recognised (A. cappari (Froggatt), A. cryptocaryae sp. n. and A. sassafras).

A newly recognised species that has been confused with the global polyphagous pest scale insect, Coccus hesperidum Linnaeus (Hemiptera: Coccomorpha: Coccidae)

“…no. BIO-21083, Bioline, Australia), PCR thermocycles and volumes of template DNA for amplifying 18S, 28S, EF-1α and wingless as per Lin et al (2017b) ( Table 2).…”

“…If the gene region was not able to be amplified by using that primer pair, then the reverse primer (HCO) was replaced by JerryR (the reverse compliment of CI-J-2183 from Simon et al 1994). Finally, because C. hesperidum s. s. was not readily amplified with either of these primer pairs, we used PcoF1 and a newly designed reverse primer HCOCh26 (Table 2), which overlaps HCO, to amplify COI from all the samples of C. hesperidum s. s. The settings of PCR mixture and used Taq-polymerase were the same as Lin et al (2017b). The successful PCR amplifications were checked by doing 1% agarose gel electrophoresis.…”

“…The sequenced PCR products of the EF-1α gene region of YPL00524 and YPL00568 included multiple amplified copies of almost identical length. To separate the multiple copies, we followed the cloning protocols described in Lin et al (2017b). Ten clones that contained the target DNA fragments were sequenced at Macrogen with the universal primer, T7 Promoter (5'-TAATACGACTCACTATAGGG -3').…”

“…Sequence editing and alignment. The computer software packages and methods using for sequence editing and alignment were as per Lin et al (2017b). Sequences of the two rRNA genes (18S and 28S) were aligned manually.…”

“…Maximum parsimony (MP) and Bayesian inference (BI) were used to estimate phylogenies. Before phylogenetic analyses, the same methods used by Lin et al (2017b) were used for checking the presence of base composition bias among taxa. The support for particular nodes from each dataset and the congruence among the hypotheses of relationships across different gene regions were assessed using bootstraps (BS) or posterior probabilities (PP), with BS ≥ 70 (Hillis & Bull 1993) and PP ≥ 0.95 (Huelsenbeck & Rannala 2004) considered to be good support.…”