A newly developed X-ray transparent ameroid constrictor for study on progression of gradual coronary stenosis

Abstract

For the experimental studies on chronic coronary stenosis, an ameroid constrictor was modified to facilitate serial cineangiographic evaluation of the degree of arterial narrowing. A radiolucent plastic ring instead of a stainless frame was used to prevent outward swelling of the ameroid material. Coronary cineangiography was performed 10, 20 and 30 days after the implantation of the modified ameroid constrictor on the left circumflex coronary artery (LCX), which could prove the progressive coronary stenosis of 57, 78 and 88% on the respective days. Collateral channels from the anterior descending branch of the left coronary artery to the LCX were demonstrated angiographically in 5 out of the 6 dogs on the 30th day.

Autologous transplantation of EPCs encoding FGF1 gene promotes neovascularization in a porcine model of chronic myocardial ischemia

“…Second, the results of the rest SPECT should be interpreted with caution. Myocardial perfusion may be influenced by the intrinsic variance of the myocardial area supplied by the LCx of each animal, the position of the ameroid constrictor and different degrees of LCx obstruction or occlusion [30]. It is more accurate to measure the reversible myocardial perfusion defect by stress SPECT in this animal model of myocardial ischemia [31].…”

Development of collateral function with repetitive coronary occlusion in a canine model reduces myocardial reactive hyperemia in the absence of significant coronary stenosis.

“…Six of eight dogs, in which fully functional collaterals had developed, were reanesthetized with the intravenous administrtion of sodium pentobarbital, and coronary cineangiography was performed with the Kifa catheter in a left anterior oblique view before coronary occlusion to evaluate the degree of coronary stenosis of the LCX due to instrumentation (Inou et al, 1980), and again at 2 minutes after the occlusion of the LCX to ascertain the developed collaterals. The x-ray tube with a 0.6-mm focal spot (Toshiba) was placed beneath the catheterization table, and the output phosphor of the image intensifier (Toshiba 6/ 9-inch II) was recorded at 42 frames/sec on 35-mm cine film (CFS 746, Kodak) loaded in an Arriflex camera.…”

Isolation, Characterization and Differentiation Potential of Cardiac Progenitor Cells in Adult Pigs

“…The most widely used porcine model of chronic ischemia has been the ameroid constrictor (Elzinga et al 1969;Inou et al 1980;O"Konski et al 1987;Roth et al 1987), which has been utilized in preclinical studies of numerous proangiogenic therapies. When the device is implanted around an artery, the constrictor absorbs water and swells, compressing the artery and leading to a progressive coronary stenosis with late occlusion with consequent myocardial ischemia and ventricular dysfunction.…”