A new xanthene‐based two‐photon fluorescent probe for the imaging of 1,4‐dithiothreitol (DTT) in living cells

Abstract

1,4-Dithiothreitol (DTT) has wide applications in cell biology and biochemistry. Development of effective methods for monitoring DTT in biological systems is important for the safe handling and study of toxicity to humans. Herein, we describe a two-photon fluorescence probe (Rh-DTT) to detect DTT in living systems for the first time. Rh-DTT showed high selectivity and sensitivity to DTT. Rh-DTT can be successfully used for the two-photon imaging of DTT in living cells, and also can detect DTT in living tissues and mice.

A Two-Photon Fluorescent Probe for the Visual Detection of Peroxynitrite in Living Cells and Zebrafish

“…A variety of fluorescent probes for detecting ONOO − have been developed and have had widespread applications [ 22 , 23 , 25 , 26 , 27 , 28 ], but there are few two-photon probes used to detect the distribution level of ONOO − in subcellular organelles. Two-photon imaging itself has the characteristics of high resolution, high throughput, noninvasiveness, and excellent imaging depth [ 29 , 30 , 31 , 32 ], which enables it to exert the dynamical visualization functions of living organisms and cells in an active state, thereby facilitating researchers in exploring the changing levels of ONOO − in cells and organisms in physiological and stimulating states.…”