A new virus-induced gene silencing vector based on Euphorbia mosaic virus-Yucatan peninsula for NPR1 silencing in Nicotiana benthamiana and Capsicum annuum var. Anaheim

Abstract

Virus-induced gene silencing is based on the sequence-specific degradation of RNA. Here, a gene silencing vector derived from EuMV-YP, named pEuMV-YP:ΔAV1, was used to silence ChlI and NPR1 genes in Nicotiana benthamiana. The silencing of the ChlI transcripts was efficient in the stems, petioles and leaves as reflected in tissue bleaching and reduced transcript levels. The silencing was stable, reaching the flowers and fruits, and was observed throughout the life cycle of the plants. Additionally, the silencing of the NPR1 gene was efficient in both N. benthamiana and Capsicum annuum. After silencing, the plants' viral symptoms increased to levels similar to those seen in wild-type plants. These results suggest that NPR1 plays a role in the compatible interactions of EuMV-YP N. benthamiana and EuMV-C. annum var. anaheim.

Ultrahigh-activity immune inducer from Endophytic Fungi induces tobacco resistance to virus by SA pathway and RNA silencing

“…They can also be highly expressed in plants with the induction of SA [22,31,32]. PR-1A and nonexpressor of PR (NPR) are the key factors in the SA pathway, involved in N. benthamiana resistance to viruses [33] or other pathogens, such as phytophthora infestations [34]. WRKYs can bind the promoter region of NPR and have functions in the SA signaling pathway [35][36][37].…”

Alteraciones anatómicas e hiperplasia inducidas por Euphorbia mosaic virus aislamiento Yucatan Peninsula en el mesófilo

“…Peninsula (EuMV-YP) es un begomovirus bipartita con una amplia gama de hospedantes experimentales. El silenciamiento génico inducido por EuMV-YP en Nicotiana benthamiana inicia en las nervaduras y se extiende hacia el resto de la hoja (Villanueva et al, 2013), lo que sugiere que es capaz de alcanzar el mesófilo. La mayoría de los begomovirus bipartitas tienen hospedantes específicos, en los cuales permanecen restringidos al floema.…”

“…En este trabajo se abordó la pregunta de si EuMV-YP es capaz de infectar las células del mesófilo de N. benthamiana y si su localización correlaciona con la severidad de los síntomas producidos. Para inocular con el virus silvestre se emplearon clonas infectivas construidas previamente (Villanueva et al, 2013), para localizar al virus se construyó el vector de expresión pEuMV-YPDAV1:GFP. Las combinaciones de plásmidos utilizados por tratamiento se hosts.…”

“…Las combinaciones de plásmidos utilizados por tratamiento se hosts. The gene silencing induced by EuMV-YP in Nicotiana benthamiana begins in the-midribs and extends to the rest of the leaf (Villanueva et al, 2013), which indicates that it is capable of reaching the mesophyll. Most bipartite begomoviruses have specific hosts in which they are restricted to the phloem.…”

In Vitro Functional Study of Rice Adenosine 5’-Phosphosulfate Kinase

“…1), and has vital cellular roles, such as maintaining intracellular redox environment, heavy metal chelation and detoxification, and disease resistance (Wang et al, 2011;Hermann et al, 2013). The activity ratio of APSK and APSR determines the partition of APS between the primary and the secondary metabolic pathways which can affect the disease and stress resistance of the plants (Xing et al, 1992;Hermann et al, 2013;Li H Q et al, 2013;Villanueva-Alonzo et al, 2013).…”