A new, versatile field immunosensor for environmental pollutantsDevelopment and proof of principle with TNT, diuron, and atrazine

Ciumasu, Ioan M.; Krämer, Petra M.; Weber, Cristina M; Kolb, Gunther; Tiemann, David; Windisch, Stefan; Frese, I.; Kettrup, A.

doi:10.1016/j.bios.2004.10.027

Cited by 56 publications

(30 citation statements)

References 36 publications

(39 reference statements)

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“…34 It was highly selective and sensitive (LOD 0.2 μg L -1 ), but it was time-consuming and very expensive, as it was based on immobilizing monoclonal antibodies on a gold surface of a single-use chip, which required one to carry out several steps (filling, washing, incubation...) before starting the measurement. a.…”

Section: Discussionmentioning

confidence: 99%

“…[31][32][33] However, to the best of authors' knowledge, up to now the only chemiluminescent method for detecting diuron has been that proposed by Ciumasu et al 34 Those authors developed a field analysis kit for a number of pollutants, including diuron, based on immobilizing monoclonal antibodies by adsorption on a gold surface of pyramidal structures. A miniaturized flow injection analysis (FIA) system was used to transport the enzymatic tracer and the sample to the single-use chip.…”

Section: Introductionmentioning

confidence: 99%

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FI-photoinduced Chemiluminescence Method for Diuron Determination in Water Samples

2011

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A new method for the determination of the herbicide diuron, using a flow injection manifold and photoinduced chemiluminescence detection, is presented. The pesticide, in basic medium, was irradiated on-line with UV light (254 nm) for 53 s. A short discussion about the possible irradiation products is included. The chemiluminescent response of the photoproducts was induced by oxidation with potassium ferricyanide in phosphate buffer at pH 11.5. The method permitted the quantification of diuron over the 0.1 -4.0 mg L -1 range, with a detection limit (S/N = 3) of 20 μg L -1 when the method was applied directly. However, the use of solid phase extraction (SPE) performed with C18 cartridges allowed us to achieve a limit of detection of 0.4 μg L -1 and a 1.5 -30 μg L -1 dynamic range. The method was successfully applied to the diuron determination in samples of water from different sources (spring, ground, mineral, irrigation, sea and tap waters) with a low consumption of reagents.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

FI-photoinduced Chemiluminescence Method for Diuron Determination in Water Samples

2011

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“…This biosensor can detect many different targets, including proteins, toxins, cells, virus, and explosives with detection limit rivaling those of the ELISA detection system. Kramer et al [28] presents a new, versatile, portable miniaturized flow-injection biosensor array which is designed for field analysis. The temperature-controlled field prototype can run for 6 h without external power supply.…”

Section: Antibody Based Biosensor Arraymentioning

confidence: 99%

Biosensor Arrays for Environmental Monitoring

Song¹,

Si²,

Yu³

et al. 2011

Environmental Monitoring

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“…Immunosensors have widespread applications in clinical diagnostics, food safety and quality control, biological analysis and environmental monitoring [1][2][3][4]. In recent past years, research in this field has evolved quickly with the aim of improving the performance of the biosensors (specificity, stability, sensitivity, detection limit).…”

Section: Introductionmentioning

confidence: 99%

Effect of electrical conditions on an impedimetric immunosensor based on a modified conducting polypyrrole

Hafaid

Chebil

Korri-Youssoufi

et al. 2010

Sensors and Actuators B: Chemical

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This work has explored the development of impedimetric immunosensors based on a conducting copolypyrrole film to which a histidine tag antibody has been bound using a complex NTA chelator coordinated with copper as metal. The electrical properties of the copolymer film have been characterized by Electrochemical Impedance Spectroscopy (EIS). The experimental study of EIS was performed over a range of dc voltages from 0 to -1.4 V (vs saturated calomel electrode SCE) where the polypyrrole layers transit from their conducting to insulating state and to demonstrate the contribution of the copper complex as redox probe. The electrical behavior of the biomaterial at these various potentials was studied according to an equivalent circuit fitting the experimental parameters. The results indicated that better immunosensor characteristics were obtained at an applied potential of -1400 mV where the reduction of copper occurs. The impedance obtained at this potential shows a linear variation of charge transfer resistance (R ct ) with the specific antigen concentration in the range of 0.1 ng mL -1 to 10 ng mL -1 with a detection limit of 0.1 ng mL -1 . Moreover, this study demonstrates that these immunosensors exhibit high sensitivity, stability and reproducibility.

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A new, versatile field immunosensor for environmental pollutantsDevelopment and proof of principle with TNT, diuron, and atrazine

Cited by 56 publications

References 36 publications

FI-photoinduced Chemiluminescence Method for Diuron Determination in Water Samples

FI-photoinduced Chemiluminescence Method for Diuron Determination in Water Samples

Biosensor Arrays for Environmental Monitoring

Effect of electrical conditions on an impedimetric immunosensor based on a modified conducting polypyrrole

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