A new vector for efficient generation of p10-single-late-promoter recombinant baculoviruses

Chaabihi, Hassan; Cêtre, Catherine; Berne, Annick

doi:10.1016/s0166-0934(96)02104-0

Cited by 4 publications

(3 citation statements)

References 13 publications

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“…The G N /G C sequence was amplified using G N Gc5’BamHI (5’-nnnnGGATCCACCATGGCAGGGATTGCAATG-3’) and G N Gc3’NotI (5’-nnnnGCGGCCGCTTATGAGGCCTTCTTAGTGG-3’) primers and inserted at the BamHI-NotI sites under the control on a second p10 promoter. The dual vector obtained, pTenTwin-N/ G N Gc, was used for cotransfection with the BacTen baculovirus DNA [30] to generate a recombinant viral clone, BacTen-N/ G N Gc, coexpressing the N and G N /G C sequences at the p10 locus.…”

Section: Methodsmentioning

confidence: 99%

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Development and validation of a pen side test for Rift Valley fever

et al. 2019

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BackgroundRift Valley fever (RVF) is one of the main vector borne zoonotic diseases that affects a wide range of ruminants and human beings in Africa and the Arabian Peninsula. A rapid and specific test for RVF diagnosis at the site of a suspected outbreak is crucial for the implementation of control measures.Methodology/Principal findingsA first-line lateral flow immunochromatographic strip test (LFT) was developed for the detection of the nucleoprotein (N) of the RVF virus (RVFV). Its diagnostic performance characteristics were evaluated using reference stocks isolates recovered from different hosts and in geographic regions mimicking clinical specimens and from known RVF negative serum samples. A high level of diagnostic accuracy (DSe (35/35), DSp (167/169)) was observed, including the absence of cross-reactivity with viruses belonging to different genera.Conclusion/SignificanceThe fact no specialized reagents and laboratory equipment are needed, make this assay a valuable, first-line diagnostic tool in resource-poor diagnostic territories for on-site RVFV detection, however the staff require training.

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Section: Methodsmentioning

confidence: 99%

“…Insect cells have been shown to be suitable for the production of RVFV proteins [30]. Bac-N and BacTen-N/G N /G C recombinant baculoviruses were amplified to generate high-titer stocks in Sf9 cells grown in HyQ-SFX serum-free medium (GE Healthcare, France).…”

Section: Methodsmentioning

confidence: 99%

Development and validation of a pen side test for Rift Valley fever

et al. 2019

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“…However, infecting insect larvae using a hemocoelic injection of a polyhedrin ‐deleted recombinant baculovirus is a costly and laborious process. In addition, feeding‐dependent infection with occlusion bodies reduces the recombinant protein yield because of competition between the polyhedrin promoter and the p10 promoter 11, 12. Thus, a method that infects insect larvae simply and efficiently with BV would facilitate the production of recombinant protein by BEVS.…”

Section: Introductionmentioning

confidence: 99%

Aerosol infectivity of a Baculovirus to Trichoplusia ni larvae: An alternative larval inoculation strategy for recombinant protein production

Jinn

Kao

Tseng

et al. 2009

Biotechnology Progress

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The baculovirus-insect expression system is a popular tool for recombinant protein production. The standard method for infecting insect larvae with recombinant baculovirus for protein production involves either feeding occlusion bodies or injecting budded virus into the cuticle. In this study, we showed that the recombinant Autographa californica multiple nucleopolyhedrovirus (AcMNPV) at titers >10(8) pfu/mL efficiently infected Trichoplusia ni (T. ni) larvae through aerosol inoculation of budded virus at a pressure of 5.5 x 10(4) Pa. The dipping T. ni larvae in virus-containing solution efficiently infected them. These results indicate that surface contamination, either by aerosol or dipping, lead to infection via spiracles. The aerosol infection route for AcMNPV was restricted to T. ni and Plutella xylostella larvae, whereas Spodoptera litura and Helicoverpa armigera larvae were resistant to this inoculation process. The yields of the reporter proteins DsRed and EGFP from T. ni larvae following aerosol infection were nearly identical to those following oral feeding or injection. This alternative baculovirus infection strategy facilitates recombinant protein and virus production by insect larvae.

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Sf9 cells: A versatile model system to investigate the pharmacological properties of G protein-coupled receptors

Schneider

Seifert

2010

Pharmacology & Therapeutics

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A new vector for efficient generation of p10-single-late-promoter recombinant baculoviruses

Cited by 4 publications

References 13 publications

Development and validation of a pen side test for Rift Valley fever

Development and validation of a pen side test for Rift Valley fever

Aerosol infectivity of a Baculovirus to Trichoplusia ni larvae: An alternative larval inoculation strategy for recombinant protein production

Sf9 cells: A versatile model system to investigate the pharmacological properties of G protein-coupled receptors

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