A New Validated RP‐ HPLC Method for the Determination of Nevirapine in Human Plasma

Abstract

A rapid, selective and sensitive high performance liquid chromatographic method for the estimation of nevirapine in human plasma has been developed. Chromatography was carried out on a Hypersil BDS C 18 column using a mixture of ammonium acetate buffer (pH 4.0 + 0.05) and acetonitrile (85:15 v/v) as the mobile phase. The eluents were monitored for the drug by UV detection at 254 nm. Oxcarbazepine was used as an internal standard for this study. The retention times for nevirapine and oxcarbazepine were found to be 7.2 and 14.7 min respectively. The method was found to be linear in the concentration range of 50 ng/mL to 5003.7 ng/mL. The method was validated as per FDA guidelines and was found to be suitable for bioequivalence and pharmacokinetic studies.

A Selective and Sensitive Method for Simultaneous Quantification of Genotoxic Impurities in Penciclovir drug substance and its dosage forms using UPLC-MS/MS

“…As selectivity of mass detectors is less prone to interference compared to UV and other non-speci ic detectors, UPLC-MS/MS method was selected for the determination of both impurities. HPLC method is available for the determination of penciclovir in plasma sample (Kumar et al, 2010). Bioequivalence study of penciclovir is reported in the literature (Pan et al, 2008).…”

Development and Validation of Stability Indicating Gradient Rp-HPLC Method for Simultaneous Estimation of Telmisartan and Chlorthalidone in Bulk Api and Fixed Dose Combination

“…[3][4][5] Literature review reveals that HPLC, [6][7][8][9][10][11] UV [12][13][14] spectrophotometric and HPTLC [15][16][17][18][19][20] methods have been reported for quantification of TEL in pharmaceutical dosage form. Chlorthalidone is chemically a 2-Chloro-5-(1-hydroxy-3-oxo-1-isoindolinyl) benzene sulfona-mide is thiazidelike diuretic.…”

Estimation of Telmisartan, Amlodipine and Chlorthalidone in Bulk and Fixed Dose Combination Using Stability Indicating High Performance Thin Layer Chromatography

“…It is official in BP [2], USP [3], EP [4] and IP [5]. A literature survey revealed that many methods are reported for the determination of TEL by spectrophotometry [6,7], HPLC [8,9], LC-MS/MS [10,11], and HPTLC [12,13].…”