A new ultrasound instrument for in vivo microimaging of mice

Foster, F. Stuart; Zhang, M.Y.; Zhou, Yuqing; Liu, G.; Mehi, J.; Chérin, Emmanuel; Harasiewicz, Kasia; Starkoski, B.G.; Zan, L.; Knapik, D.A.; Adamson, S. Lee

doi:10.1016/s0301-5629(02)00567-7

Cited by 317 publications

(193 citation statements)

References 31 publications

Supporting

Mentioning

191

Contrasting

Order By: Relevance

“…Mouse fetal echocardiography has been carried out using standard clinical ultrasound systems (Gui et al 1996;Spurney et al 2004), as well as ultra-high frequency ultrasound systems, also referred to as ultrasound biomicroscopes (Srinivasan et al 1998;Phoon et al 2000Phoon et al , 2004Foster et al 2002;Zhou et al 2002Zhou et al , 2003. Ultra high frequency ultrasound systems equipped with 30-40 MHz transducers have greatly improved 2D spatial resolution that allow even very early mouse embryos (from embryonic day 5.5) to be imaged (Srinivasan et al 1998;Phoon et al 2000;Foster et al 2002).…”

Section: Introductionmentioning

confidence: 99%

“…Ultra high frequency ultrasound systems equipped with 30-40 MHz transducers have greatly improved 2D spatial resolution that allow even very early mouse embryos (from embryonic day 5.5) to be imaged (Srinivasan et al 1998;Phoon et al 2000;Foster et al 2002). However, the higher ultrasound frequencies cause reduced penetration depth, making it more difficult to obtain optimal ultrasound imaging planes for cardiac assessments (Zhou et al 2003).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Cardiovascular Assessment of Fetal Mice by In Utero Echocardiography

Leatherbury

Tian

et al. 2008

Ultrasound in Medicine & Biology

View full text Add to dashboard Cite

To establish a developmental profile of fetal mouse cardiovascular parameters, we analyzed a large body of ultrasound measurements obtained by in utero echocardiography of C57BL/6J fetal mice from embryonic day 12.5 to 19.5 (term). Measurements were obtained using 2D, spectral Doppler and M-mode imaging with standard clinical cardiac ultrasound imaging planes. As these studies were conducted as part of a large scale mouse mutagenesis screen, stringent filtering criteria were used to eliminate potentially abnormal fetuses. Our analysis showed heart rate increased from 190 to 245 bpm as the mouse fetus grew from 8 mm at embryonic day 12.5 to 18.7 mm at term. This was accompanied by increases in peak outflow velocity, E-wave, E/A ratio and ventricular dimensions. In contrast, the A-wave, myocardial performance index and isovolemic contraction time decreased gradually. Systolic function remained remarkably stable at 80% ejection fraction. Analysis of intra and interobserver variabilities showed these parameters were reproducible, with most comparing favorably to clinical ultrasound measurements in human fetuses. A comprehensive database was generated comprising 23 echocardiographic parameters delineating fetal mouse cardiovascular function from embryonic day 12.5 to term. This database can serve as a standard for evaluating cardiovascular pathophysiology in genetically altered and mutant mouse models.

show abstract

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Cardiovascular Assessment of Fetal Mice by In Utero Echocardiography

Leatherbury

Tian

et al. 2008

Ultrasound in Medicine & Biology

View full text Add to dashboard Cite

show abstract

“…Although several studies in the 1970s and 1980s had tested the toxicity of ultrasound wave exposure in mouse embryos, this was the first study to show that UBM could be used to compare morphological features during development. Further developments in high-frequency ultrasound led produced instruments suitable for routine mouse imaging (Turnbull et al, 1995b;Foster et al, 2002), and UBM systems became commercially available in 2002 (Visualsonics, Inc., www. visualsonics.com), providing availability to more researchers.…”

Section: Ultrasound Imaging Of Mouse Developmentmentioning

confidence: 99%

Multimodal imaging of mouse development: Tools for the postgenomic era

Dickinson

2006

Developmental Dynamics

View full text Add to dashboard Cite

With the sequence of the mouse genome known, it is now possible to create or identify mutations in every gene to determine the molecules necessary for normal development. Consequently, there is a growing need for advanced phenotyping tools to best understand defects produced by altering gene function. Perhaps nothing is more satisfying than to directly observe a process in action; to disturb it and see for ourselves how the process changes before our very eyes. No doubt, this desire is what drove the invention of the very first microscopes and continues to this day to fuel progress in the field of biological imaging. Because mouse embryos are small and develop embedded within many tissue layers within the nurturing environment of the mother, directly observing the dynamic, micro-and nanoscopic events of early mammalian development has proven to be one of the greater challenges for imaging scientists. Here, I will review some of the imaging methods being used to study mouse development, highlighting the results obtained from imaging.

show abstract

“…In the decade since UBM was first introduced for imaging mouse embryos (Turnbull et al 1995), UBM technology has progressed significantly in terms of higher image frame-rates (currently up to ~100 frames/s), multiple imaging frequencies (over the 30-60 MHz range) and newer digital image processing methods (e.g., Foster et al 2002;Goertz et al 2003). Nevertheless, current UBM systems continue to be based on single-element focused polyvinylidene difluoride (PVDF) transducers, much the same as those described in the original UBM systems (Sherar and Foster 1989).…”

Section: Introductionmentioning

confidence: 99%

40-MHz annular array imaging of mouse embryos

Aristizábal

Ketterling

Turnbull

2006

Ultrasound in Medicine & Biology

View full text Add to dashboard Cite

Ultrasound biomicroscopy (UBM) has emerged as an important in vivo imaging approach for analyzing normal and genetically-engineered mouse embryos. Current UBM systems use fixed-focus transducers, which are limited in depth-of-focus. Depending on the gestational age of the embryo, regions of interest in the image can extend well beyond the depth-of-focus for a fixed-focus transducer. This shortcoming makes it particularly problematic to analyze 3D datasets and to generate accurate volumetric renderings of the mouse embryonic anatomy. To address this problem, we have developed a 5-element, 40-MHz annular array transducer and a computer controlled system to acquire and reconstruct fixed-and array-focused images of mouse embryos. Both qualitative and quantitative comparisons showed significant improvement with array-focusing including an increase of 3-9 dB in signal-to-noise ratio and an increase of at least 2.5-mm in depth-of-focus. Volumetric rendered images of brain ventricles demonstrated the clear superiority of array-focusing for 3D analysis of mouse embryonic anatomy.

show abstract

A new ultrasound instrument for in vivo microimaging of mice

Cited by 317 publications

References 31 publications

Cardiovascular Assessment of Fetal Mice by In Utero Echocardiography

Cardiovascular Assessment of Fetal Mice by In Utero Echocardiography

Multimodal imaging of mouse development: Tools for the postgenomic era

40-MHz annular array imaging of mouse embryos

Contact Info

Product

Resources

About