A new type of synthetic peptide library for identifying ligand-binding activity

Abstract

Our aim was to improve techniques for drug development by facilitating the identification of small molecules that bind with high affinity to acceptor molecules (for example, cell-surface receptors, enzymes, antibodies) and so to mimic or block their interaction with the natural ligand. Previously such small molecules have been characterized individually on a serial basis. The systematic synthesis and screening of peptide libraries of defined structure represents a new approach. For relatively small libraries, predetermined sequence variations on solid-phase supports have been used, and large libraries have been produced using a bacteriophage vector into which random oligodeoxynucleotide sequences have been introduced, but these techniques have severe limitations. Here we investigate an alternative approach to synthesis and screening of peptide libraries. Our simple methodology greatly enhances the production and rapid evaluation of random libraries of millions of peptides so that acceptor-binding ligands of high affinity can be rapidly identified and sequenced, on the basis of a 'one-bead, one-peptide' approach.

Assessing the inhibitory potency of galectin ligands identified from combinatorial (glyco)peptide libraries using surface plasmon resonance spectroscopy

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Modular peptides from the thermoplastic squid sucker ring teeth form amyloid-like cross-β supramolecular networks

“…A combinatorial peptide macro array interaction assay [10,[26][27][28][29] with a suckerin-peptide based library was designed in order to obtain a preliminary screening of peptide interactions and to identify binding interactions of binary peptide combinations ( Figure 1B, details of the assay are described in Materials and methods section). All binary combinations of peptides were screened at three different pHs and three analyte concentrations to generate 441 different conditions.…”

RNA and DNA aptamers in cytomics analysis

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