A new type F Wolbachia from Splendidofilariinae (Onchocercidae) supports the recent emergence of this supergroup

Lefoulon, Emilie; Gavotte, Laurent; Junker, Kerstin; Barbuto, Michela; Uni, Shigehiko; Landmann, Frédéric; Laaksonen, Sauli; Saari, Seppo; Nikander, Sven; Lima, Sueli de Souza; Casiraghi, Maurizio; Bain, O.; Martin, Coralie

doi:10.1016/j.ijpara.2012.09.004

Cited by 45 publications

(74 citation statements)

References 56 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Some F Wolbachia strains are obligatorily associated with Mansonella spp. and other filarial nematodes (29)(30)(31). However, they were placed in distinct lineages from the arthropod-associated F Wolbachia strains including wCle, although statistical supports for the groupings were not necessarily significant (Fig.…”

Section: Conserved Biotin Operon Inserted In the Wolbachia Genomementioning

confidence: 96%

Evolutionary origin of insect– Wolbachia nutritional mutualism

Nikoh

Hosokawa

Moriyama

et al. 2014

Proc. Natl. Acad. Sci. U.S.A.

364

407

View full text Add to dashboard Cite

Obligate insect-bacterium nutritional mutualism is among the most sophisticated forms of symbiosis, wherein the host and the symbiont are integrated into a coherent biological entity and unable to survive without the partnership. Originally, however, such obligate symbiotic bacteria must have been derived from free-living bacteria. How highly specialized obligate mutualisms have arisen from less specialized associations is of interest. Here we address this evolutionary issue by focusing on an exceptional insect-Wolbachia nutritional mutualism. Although Wolbachia endosymbionts are ubiquitously found in diverse insects and generally regarded as facultative/parasitic associates for their insect hosts, a Wolbachia strain associated with the bedbug Cimex lectularius, designated as wCle, was shown to be essential for host's growth and reproduction via provisioning of B vitamins. We determined the 1,250,060-bp genome of wCle, which was generally similar to the genomes of insect-associated facultative Wolbachia strains, except for the presence of an operon encoding the complete biotin synthetic pathway that was acquired via lateral gene transfer presumably from a coinfecting endosymbiont Cardinium or Rickettsia. Nutritional and physiological experiments, in which wCle-infected and wCle-cured bedbugs of the same genetic background were fed on B-vitaminmanipulated blood meals via an artificial feeding system, demonstrated that wCle certainly synthesizes biotin, and the wCleprovisioned biotin significantly contributes to the host fitness. These findings strongly suggest that acquisition of a single gene cluster consisting of biotin synthesis genes underlies the bedbug-Wolbachia nutritional mutualism, uncovering an evolutionary transition from facultative symbiosis to obligate mutualism facilitated by lateral gene transfer in an endosymbiont lineage.

show abstract

Section: Conserved Biotin Operon Inserted In the Wolbachia Genomementioning

confidence: 96%

Evolutionary origin of insect– Wolbachia nutritional mutualism

Nikoh

Hosokawa

Moriyama

et al. 2014

Proc. Natl. Acad. Sci. U.S.A.

364

407

View full text Add to dashboard Cite

show abstract

“…An intriguing hypothesis suggests that this group is a basal branching lineage that might represent Wolbachia's ancestral lifestyle 16 . While phylogenetic analyses of Wolbachia strains based on a single or a few genes usually enable correct supergroup assignments, relationships between supergroups remain poorly resolved and consequently, partially conflicting phylogenetic hypotheses were proposed 11,[17][18][19][20] . Furthermore, these data sets are especially prone to artefacts caused by recombination between Wolbachia strains 21 .…”

mentioning

confidence: 99%

Phylogenomic analyses uncover origin and spread of the Wolbachia pandemic

et al. 2014

View full text Add to dashboard Cite

Of all obligate intracellular bacteria, Wolbachia is probably the most common. In general, Wolbachia are either widespread, opportunistic reproductive parasites of arthropods or essential mutualists in a single group of filarial nematodes, including many species of medical significance. To date, a robust phylogenetic backbone of Wolbachia is lacking and consequently, many Wolbachia-related phenomena cannot be discussed in a broader evolutionary context. Here we present the first comprehensive phylogenomic analysis of Wolbachia supergroup relationships based on new whole-genome-shotgun data. Our results suggest that Wolbachia has switched between its two major host groups at least twice. The ability of some arthropod-infecting Wolbachia to universally infect and to adapt to a broad range of hosts quickly is restricted to a single monophyletic lineage (containing supergroups A and B). Thus, the currently observable pandemic has likely a single evolutionary origin and is unique within the radiation of Wolbachia strains.

show abstract

“…Because filarioid nematodes are difficult to collect, their diversity is mostly unknown, with only a few relatively recent studies reporting new species such as Pelecitus nematides from the Tehuantepec jackrabbit (Lepus flavigularis) ( Jiménez-Ruiz et al, 2004) and Diplotriaena railliet in birds such as Passer domesticus and P. pyrhonotus from Pakistan ( Chandio et al, 2015). Genetic studies were conducted for Micipsella numidica in the hare Lepus europaeus from Italy ( Gabrielli et al, 2015) and Rumenfilaria andersoni along with other 48 species of onchocercid subfamilies of filarial nematodes ( Lefoulon et al, 2012 andLefoulon et al, 2015). Regardless of our ability to assign the obtained filarioid DNA sequences to a given species or genus, reports such as these do suggest that diverse Filarioidea nematodes may be widely distributed in Europe, in a large range of hosts.…”

Section: Discussionmentioning

confidence: 99%

Culex pipiens as a potential vector for transmission of Dirofilaria immitis and other unclassified Filarioidea in Southwest Spain

Bravo‐Barriga

Parreira

Almeida

et al. 2016

Veterinary Parasitology

View full text Add to dashboard Cite

Dirofilaria immitis is one of the most frequently detected mosquito-transmitted zoonotic filarioid nematode in mammals in Europe, being canine dirofilariosis a major animal health problem, endemic in the Mediterranean area. This study, focused on Southwest Spain, in order to bring new insights into (i) the epidemiology of Dirofilaria spp., (ii) the species of Culicid vectors possibly involved in their transmission and (iii) the genetic variability of those potential vectors. A total of 881 adult female mosquitoes from 11 different species, were captured during 2012-2013, and detection of filarioid DNA was attempted by PCR using specific primers (ITS-2 and COI), followed by DNA sequencing. In a single Culex pipiens specimen D. immitis DNA was detected both in the head-thorax and abdomen sections. Filarioid nematode DNA was also detected in eight additional Cx. pipiens specimens also in both the thorax and the abdomen, but analysis of sequence data did not allow unambiguous assignment of any of the obtained sequences to a previously defined species. All Cx. pipiens with filarioid DNA were individually analysed by CQ11 to discriminate between pipiens, molestus, and hybrid forms. Besides, rDNA ITS-2 sequence analysis revealed the presence of haplotype H1 and H2 of Cx. pipiens. To our knowledge this study revealed, for the first time in Spain, the occurrence of likely mature infection of D.1 immitis in Cx. pipiens, as well as with other yet uncharacterized nematodes, supporting its role as a potential vector of these filarids.

show abstract

A new type F Wolbachia from Splendidofilariinae (Onchocercidae) supports the recent emergence of this supergroup

Cited by 45 publications

References 56 publications

Evolutionary origin of insect– Wolbachia nutritional mutualism

Evolutionary origin of insect– Wolbachia nutritional mutualism

Phylogenomic analyses uncover origin and spread of the Wolbachia pandemic

Culex pipiens as a potential vector for transmission of Dirofilaria immitis and other unclassified Filarioidea in Southwest Spain

Contact Info

Product

Resources

About