A New Trisaccharide, 3<sup>G</sup>-α-<scp>d</scp>-Glucosyl-sucrose, Synthesized by the Transglucosidase Action of Immobilized Buckwheat α-Glucosidase

Chiba, Seiya; Asada-Komatsu, Yoko; Kimura, Atsuo; Kawashima, Koji

doi:10.1080/00021369.1984.10866291

Cited by 2 publications

(2 citation statements)

References 3 publications

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“…The anomeric form of glucose produced was quantitatively determined from the ratio of the area (or height) of the peak of oc-or fJ-glucose to that of the peak of arabitol, whose calibration curve was indicated in the previous paper. 9) A TMS-derivative of oc-isomaltose was prepared from the hydrolysis products of 2-oc-isomaltosyl-fJ-fructofuranoside 25 ) by Candida utilis invertase (fJ-D-fructofuranosidase, Seikagaku Kogyo Co., Ltd.) according to the same method already mentioned. The gas-liquid chromatogram of the TMS-derivative of oc-isomaltose is …”

Section: Methodsmentioning

confidence: 99%

Quantitative Study of the Anomeric Forms of Isomaltose and Glucose Produced by Isomalto-dextranase and Glucodextranase

Takayanagi

Okada

Chiba

1987

Agricultural and Biological Chemistry

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Section: Methodsmentioning

confidence: 99%

Quantitative Study of the Anomeric Forms of Isomaltose and Glucose Produced by Isomalto-dextranase and Glucodextranase

Takayanagi

Okada

Chiba

1987

Agricultural and Biological Chemistry

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“…The a-G2-(1?2)-a-G1-(1?2)-b-Fru trisaccharide has been previously described (Fischer et al, 2006), but no matching with the reported NMR data was found (Table S1). The a-G2-(1?3)-a-G1-(1?2)-b-Fru trisaccharide has also been described (Chiba et al, 1984), but no NMR data were published. Our NMR data for the major product fit well with those described for the a-G2-(1?4)-a-G1-(1?2)-b-Fru (Erlose) (Wei et al, 1997).…”

Section: Identification and Structural Characterization Of The Transfmentioning

confidence: 99%

Efficient production of isomelezitose by a glucosyltransferase activity in Metschnikowia reukaufii cell extracts

García-González

Plou

Cervantes

et al. 2019

Microbial Biotechnology

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Summary Metschnikowia reukaufii is a widespread yeast able to grow in the plants’ floral nectaries, an environment of extreme conditions with sucrose concentrations exceeding 400 g l−1, which led us into the search for enzymatic activities involved in this sugar use/transformation. New oligosaccharides were produced by transglucosylation processes employing M. reukaufii cell extracts in overload‐sucrose reactions. These products were purified and structurally characterized by MS‐ESI and NMR techniques. The reaction mixture included new sugars showing a great variety of glycosidic bonds including α‐(1→1), α‐(1→3) and α‐(1→6) linkages. The main product synthesized was the trisaccharide isomelezitose, whose maximum concentration reached 81 g l−1, the highest amount reported for any unmodified enzyme or microbial extract. In addition, 51 g l−1 of the disaccharide trehalulose was also produced. Both sugars show potential nutraceutical and prebiotic properties. Interestingly, the sugar mixture obtained in the biosynthetic reactions also contained oligosaccharides such as esculose, a rare trisaccharide with no previous NMR structure elucidation, as well as erlose, melezitose and theanderose. All the sugars produced are naturally found in honey. These compounds are of biotechnological interest due to their potential food, cosmeceutical and pharmaceutical applications.

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A New Trisaccharide, 3^G-α-d-Glucosyl-sucrose, Synthesized by the Transglucosidase Action of Immobilized Buckwheat α-Glucosidase

Cited by 2 publications

References 3 publications

Quantitative Study of the Anomeric Forms of Isomaltose and Glucose Produced by Isomalto-dextranase and Glucodextranase

Quantitative Study of the Anomeric Forms of Isomaltose and Glucose Produced by Isomalto-dextranase and Glucodextranase

Efficient production of isomelezitose by a glucosyltransferase activity in Metschnikowia reukaufii cell extracts

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A New Trisaccharide, 3G-α-d-Glucosyl-sucrose, Synthesized by the Transglucosidase Action of Immobilized Buckwheat α-Glucosidase

Cited by 2 publications

References 3 publications

Quantitative Study of the Anomeric Forms of Isomaltose and Glucose Produced by Isomalto-dextranase and Glucodextranase

Quantitative Study of the Anomeric Forms of Isomaltose and Glucose Produced by Isomalto-dextranase and Glucodextranase

Efficient production of isomelezitose by a glucosyltransferase activity in Metschnikowia reukaufii cell extracts

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A New Trisaccharide, 3^G-α-d-Glucosyl-sucrose, Synthesized by the Transglucosidase Action of Immobilized Buckwheat α-Glucosidase