A new triple system DNA-Nanosilver-Berberine for cancer therapy

Abstract

The isoquinoline quaternary alkaloid Berberine possesses a variety of pharmacological properties that suggests its promising application for an anticancer delivery system design utilizing its ability to intercalate DNA. In the current work we have investigated the effects of Berberine on the human T-cell leukemia cell line in vitro. Fluorescent microscopy of leukemic cells revealed Berberine nuclear localization. The results showed that Berberine inhibited leukemic cell growth in a time-and dose-dependent manner, that was associated with reactive oxygen species production intensification and caspase 3/7 activity increase with followed apoptosis induction. Berberine was used as a toxic and phototoxic agent for triple system synthesis along with DNA as a carrier and nanosilver as a plasmonic accelerator of Berberine electronic transitions and high energy emission absorbent centers. The proposed method allows to obtain the complex of DNA with Berberine molecules and silver nanopoarticles. The optical properties of free components as well as their various combinations, including the final triple system DNA-Nanosilver-Berberine, were investigated. Obtained results support the possibility to use the triple system DNA-Nanosilver-Berberine as an alternative therapeutic agent for cancer treatment.

C60 Fullerene as an Effective Nanoplatform of Alkaloid Berberine Delivery into Leukemic Cells

“…Ber applications as a low-cost therapeutic with anti-inflammatory, antimutagenic [8], antidiabetic [9], antimicrobial, and antiviral effects seem to be promising [4,10]. In recent years, Ber has been reported to inhibit the proliferation of many cancer cell lines originated from head and neck squamous carcinoma [11], melanoma [12], leukemia [3,13,14], oral [15], pancreatic [16], colon [17], breast [18], and prostate cancer [19]. Given the nitrogen atom positive charge [20], Ber interacts directly with genomic [21,22] and telomeric [23,24] DNA, inducing double-strand breaks, and telomere stabilization, respectively.…”

“…The other potential intracellular targets of Ber are DNA topoisomerase I, POT1 [3], Wnt [17,25], p53 [18,26], NF-kB [27], cyclooxygenase-2, Mcl-1 [15], nucleophosmin/B23 [13], and death-domain-associated protein [28]. Ber’s anticancer cytotoxicity is associated mainly with oxidative stress escalation and mitochondrial dysfunction [7,16,19], apoptosis activation, and cell cycle arrest [10,14,15,18,28].…”

Temperature and pH Dual Responsive Nanogels of Modified Sodium Alginate and NIPAM for Berberine Loading and Release

“…Grebinyk and his research group designed a drug delivery system that combines berberine with a DNA carrier to treat leukemia. 23 …”

Rapid and visual detection of berberine hydrochloride based on a water‐soluble pyrene derivative

“…Berberine hydrochloride (BRH) is a type of isoquinoline alkaloid that exists in many medicinal plants such as Coptis chinensis , barberry root and Chelidonium majus . It has been shown to be a bioactive natural component with anti‐tumor, anti‐tuberculosis, anti‐bacterial and anti‐oxidant activities.…”