A new transposable element inChironomus thummi

Wobus, Ulrich; Bäumlein, Helmut; Богачев, С. С.; Borisevich, I. V.; Panitz, Reinhard; Kolesnikov, N. N.

doi:10.1007/bf00633834

Cited by 16 publications

(4 citation statements)

References 16 publications

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“…These observations are consistent with an interpretation of the paternal components as transposable elements. Thus far, five transposable elements have been described in Chironomus thummi (Schmidt, 1984;Bogachev et al, 1986;Hankeln & Schmidt, 1990;Wobus et al, 1990). However, it is unlikely that these known elements are responsible either for the Rud or for the HLE syndrome, since no abnormalities have been reported from the chironomids on which the above studies on transposable elements were done.…”

Section: Discussionmentioning

confidence: 88%

The HLE hybrid dysgenesis syndrome in the midgeChironomus thummi: Differences in the dysgenic potential between strains

Hägele

1995

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After crossing experiments between two Chironomus subspecies the HLE hybrid dysgenesis syndrome occurs non-reciprocally in the F1 hybrids if the males of each of the four Ch. th. thummi strains (HL, So, G, Kr) are crossed to females of Ch. th. piger, strain E. In hybrid egg masses, differences in the strength of the abnormal trait 'reduced egg hatch' were found between the differentpiger E ? x thummi c~ interstrain crosses. From the results it is concluded that each thummi strain has a specific hybrid dysgenesis potential which determines the severity of the HLE syndrome in the hybrids. The HLE syndrome occurs also in the hybrids of crosses of different Ch. th. thummi strains. In these hybrids the syndrome is only obtained if the male parent descends from a strain which has a higher HLE hybrid dysgenesis potential than that strain from which the female parent is derived. These results demonstrate that the HLE syndrome is not restricted to the hybrids of the thummi x piger subspecies cross. In the Ch. th. thummi interstrain crosses the hybrids of that cross direction are normal, which is reciprocal to the cross direction affected by the HLE syndrome. This is in contrast to the thummi x piger subspecies crosses, where the hybrids of one cross direction always show the HLE syndrome and the hybrids of the reciprocal cross direction the Rud syndrome. Thus, the present study also demonstrates that the HLE and Rud hybrid dysgenesis syndrome in Chironomus are independent from one another.

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Section: Discussionmentioning

confidence: 88%

The HLE hybrid dysgenesis syndrome in the midgeChironomus thummi: Differences in the dysgenic potential between strains

Hägele

1995

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“…This A2G5 pattern is not universal to all short inverted repeattype elements. Other elements that conform to this pattern (apart from the hA T family members discussed above), include the Ispr element from Pisum sativum (pea) (Bhattacharyya et al 1990), Tpcl from Petroselinum crispum (Parsley) (Herrmann, Schulz & Hahlbrock, 1988), 1723 from Xenopus laevis (Kay & Dawid, 1983), and TECthl from Chironomus thummi (Wobus et al 1990). Interestingly, all these elements generate 8 bp target site duplications upon transposition.…”

Section: (Iv) Hermes Terminal and Subterminal Sequencesmentioning

confidence: 91%

TheHermestransposable element from the house fly,Musca domestica, is a short inverted repeat-type element of thehobo, Ac, andTam3(hAT) element family

1994

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SummaryThe genome of the house fly, Musca domestica, contains an active transposable element system, called Hermes. Using PCR and inverse PCR we amplified and sequenced overlapping segments of several Hermes elements and from these data we have constructed a 2749 bp consensus Hermes DNA sequence. Hermes termini are composed of 17 bp imperfect inverted repeats that are almost identical to the inverted terminal repeats of the hobo element of Drosophila melanogaster. Full length Hermes elements contain a single long ORF capable of encoding a protein of 612 amino acids which is 55% identical to the amino acid sequence of the hobo transposase. Comparison of the ends of the Hermes and hobo elements to those of the Ac element of Zea mays, and the Tam3 element of Antirrhinum majus, as well as several other plant and insect elements, revealed a conserved terminal sequence motif. Thus Hermes is clearly a member of the hobo, Ac and Tam3 (hAT) transposable element family, other members of which include the Tagl element from Arabidopsis thaliana and the Bg element from Zea mays. The evolution of this class of transposable elements and the potential utility of Hermes as a genetic tool in M. domestica and related species are discussed.

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“…Besides, the mobile (transposable) elements (ME) were revealed in BRs of Chironomus thummi, namely MEC and TEC (BOGACHEV eta/., 1990;WOBUS et al, 1990;BLINOV et al, 1991). The new type of ME, designated as C6.10, was localized in BRa and BRb of C. thummi along with dispersed location on the chromosomes.…”

Section: Introductionmentioning

confidence: 97%

Homology of Balbiani rings among chironomid species and localization of a new mobile element on the polytene chromosomes

Filippova

Kiknadze

Aimanova

et al. 1992

Netherlands Journal of Aquatic Ecology

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The results of in situ cross-hybridization of the cloned DNA fragments of BRa, BRb and BRc from Chironomus thummito the polytene chromosomes of 14 Chironomus species and Camptochironomus tentans are presented. BRs of the species studied were demonstrated to contain the homologous DNA sequences. The cloned fragment from the BRa of C. thummi hybridized with the BRa of C. pigerand with a region on the arm G of C. tentans and C. plumosus; the latter species had no extra BR. The clone ~.16 from the BRb of C. thummi hybridized only with the developed BR on the arm G of all species studied. The sequence from the BRc of C. thummiwas located in the BRc of C. pigerand in the developed BR of C. plumosus and C. nuditarsis, which were located at the most distal end of arm G. These clones can be used as markers of homologous BRs. The new mobile elements C6.10 from C. thummi genome were localized on the polytene chromosomes of 10 Chironomus species and Camptochironomus tentans. The species of the genera Lipiniella, Cryptochironomus and G/yptotendipes did not contain the sequences homologous to ME C6.10.

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A new transposable element inChironomus thummi

Cited by 16 publications

References 16 publications

The HLE hybrid dysgenesis syndrome in the midgeChironomus thummi: Differences in the dysgenic potential between strains

The HLE hybrid dysgenesis syndrome in the midgeChironomus thummi: Differences in the dysgenic potential between strains

TheHermestransposable element from the house fly,Musca domestica, is a short inverted repeat-type element of thehobo, Ac, andTam3(hAT) element family

Homology of Balbiani rings among chironomid species and localization of a new mobile element on the polytene chromosomes

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